A sensitive and selective reversed-phase HPLC method for analysis of ketorolac in aqueous and vitreous humor from the human
eye has been developed and validated. Chromatographic separation was achieved on a 250mm ×4.6mm i.d., 5-μm particle, C18 analytical column. Photo diode-array detection was performed at 314nm. Response was a linear function of ketorolac concentration
from 10 to 800ngmL−1. The limits of detection (LOD) and quantification (LOQ) were 3.0 and 10ngmL−1, respectively. Intra-day and inter-day bias were less than 2.05 and 2.28%, respectively, and intra-day and inter-day RSD
were no higher than 3.60 and 5.80%, respectively. Fluid obtained from patients eyes’ after topical application of Acular eye
drops before retina decolman surgery was analyzed by use of the method. The method enabled successful quantification of levels
of ketorolac in aqueous and vitreous humor.