In the normal form of Mammillaria elongata, shoots were regenerated in vitro, through callus, from tubercle explants excised from the upper part of the branch and cultured on Murashige and Skoog medium
(MS) with 1.07 μM α-napthaleneacetic acid (NAA) and 22.20 μM 6-benzylaminopurine (BA). A high percentage of tubercles explants
of the M. elongata cristate form, excised from the tip of the branch and cultured on MS with 0.54 μM NAA and 0.44 μM BA or 1.07 μM NAA, responded
by initially forming an inflated cristate shoot, which gave cristate and normal shoots, without callus intervention, when
transferred on basal MS. Callus formed on cristate tubercles explants gave both cristate and normal shoots when transferred
onto basal MS. Normal and cristate shoots were rooted in vitro on MS with 9.84 μM or 0.98 μM indole-3-butyric acid, respectively, and established ex vitro. In both normal and cristate form, the differential response appeared to be associated with the site of the explant excision.
The formation of shoots was influenced by the season of culture; i.e., explants excised in October had a higher shoot formation
rate than those excised February.