Micropropagation of grey mangrove Avicennia marina

King Faisal University
Plant Cell Tissue and Organ Culture (Impact Factor: 2.13). 12/2002; 72(1):87-93. DOI: 10.1023/A:1021205731719


This study was conducted to develop a micropropagation protocol for grey mangrove, Avicennia marina (Forsk.) Vierh., a marine halophytic woody plant. Nodal explants were cultured on MS medium supplemented with both 6-furfurylaminopurine (kinetin) and 6-benzylaminopurine (BA) at 0, 0.5, 1, or 2 mg 1-1 combined with 0, 0.01, 0.1, or 1 mg 1-1 indole-3-butyric acid (IBA). Single shoots grew from either one or both of the preexisting axillary buds in a nodal explant. Nodes cultured on a treatment consisting of 1 mg 1-1 kinetin combined with 0.5 mg 1-1 BA, with no IBA, gave the highest percentage of shoot formation, 75%. The addition of IBA was inhibitory to shoot formation, particularly at concentrations above 0.01 mg 1-1. The growth of resultant shoots was relatively slow requiring 4 months to grow a couple of centimeters. Maximum shoot elongation occurred in response to a combination of 0.01 mg 1-1 IBA, 2 mg 1-1 kinetin, and 0.5 mg 1-1 BA. Resultant shoots were rooted on a medium containing either IBA, indole-3-acetic acid (IAA), or α-naphthaleneacetic acid (NAA) at 0.5, 1, 2, or 4 mg 1-1. Above 95% rooting was obtained on 0.5 mg 1-1 of either IBA or NAA. The highest root number, 2.2 roots per shoot, was observed on 4 mg 1-1 IBA. Root elongation was best, 15.4 mm, on 2 mg 1-1 IBA. Plantlets survived in soil and produced new shoot and root growth.

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    • "The SPAR techniques have been employed extensively in characterization of micropropagated plantlets.[10À16] Earlier, a study was conducted by Al-Bahrany and Al- Khayri [17] to obtain in vitro plantlets of A. marina, but there is still no report on the molecular characterization of micropropagated plants. However, the developed method is meager and gave a low number of shoots. "
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    ABSTRACT: Okla (2015): Clonal in vitro multiplication of grey mangrove and assessment of genetic fidelity using single primer amplification reaction (SPAR) methods, Biotechnology & Biotechnological Equipment,
    Full-text · Article · Aug 2015 · Biotechnology & Biotechnological Equipment
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    • "Such levels of destruction and habitat fragmentation raise concerns about the conservation of mangrove diversity. To augment conservation, management efforts to germinate and especially for unique genotypes, has to be made [1]. "
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    ABSTRACT: Excoecaria agallocha L. is a critically endangered mangrove tree from the Pichavaram mangrove reserve forest, the Tamil Nadu Coastal area. It is distributed on the seashore and the edge-mangrove. In order to reduce the decrease in number of these Mediterranean mixed stand, unsupervised forest management practices have drastically been reduced. In addition, the deforestation of the mangrove area, along with a low seed germination rate further endanger this species. In this study we developed a protocol for the micropropagation of adult Excoecaria agallocha. Microcuttings were obtained from lateral and apical twigs of mature plants and used as explants. Microcuttings with axillary buds were grown on different media, plant growth regulators and phenolic exudation substances. The axillary shoots produced on uncontaminated explants were excised, segmented and recultured in the same medium, to increase the stock of shoot cultures. The Modified Murashige and Skoog (MMS) medium, augmented with different concentrations of N 6 – benzyl adenine (BAP) and Naphthalene acetic acid (NAA), either alone, or in combinations, as a potential medium for shoot multiplication by nodal segments, was tested. In the following experiment, equal molar concentrations of four cytokonins [BAP, Kinetin and 2-isopenthenyladenine (2iP)] in combination with equal molar concentrations of three auxins [ NAA, Indole acetic acid (IAA) and indole-3-butyric ]were used to test the rate of axillary shoot proliferation, induced on MMS agar medium supplemented with 3.9 µM BAP and 1.34 µM NAA after 6 weeks in culture. Different auxins (NAA, IBA and IAA) were to determine the optimum conditions for in vitro rooting of microshoots. The best results were accomplished with NAA 5.41 µM (89% rooting) and with IBA at 2.85 or 5.71µM (86% and 86.5%) rooting, respectively).
    Full-text · Article · Jan 2012 · International Journal of Conservation Science
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    • "In the reforestation, A. alba can grow in the most seaside area (Robertson et al. 1992). Suspension culture of Avicenniaceae has been unsuccessful, although nodular structures from axillary buds cultured in Murashige and Skoog's (MS) (Murshige and Skoog 1962) basal medium, have been successfully used for micropropagation of A. marina (Saenger 2002, Al-Bahrany and Al-Khayri 2003). In this study, we established cell suspension cultures of A. alba. "
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    ABSTRACT: The effects of salts on cell proliferation in suspension cultures of Avicennia alba and on callus induction from the leaf-tissue of A. marina were investigated using a small-scale liquid culture method. The effects of the seawater components, NaCl, KCl, CaCl2, MgCl2, and MgSO4 were examined separately. In both Avicennia species, the cell growth was increased in the presence of a low concentration, 10 mM, of MgCl2. Even in the presence of 100 mM NaCl, growth was stimulated in A. marina and there was no inhibition of growth in A. alba. CaCl2 was the most inhibitory and completely inhibited growth at 100 mM in both species. Similarities and differences in the effects of sea salts among Avicennia species and Sonneratia alba and Bruguiera sexangula of different mangrove families are discussed. This is the first report on establishing cell suspension culture of the mangrove plant A. alba belonging to the Avicenniaceae.
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