Susceptibility of hairy root lines of Brassica species to Plasmodiophora brassicae and in an in vitro subculture system. J Gen Plant Pathol
To investigate the susceptibility of hairy root lines of Brassica species to Plasmodiophora brassicae, hairy roots were induced in a number of Brassica species with Agrobacterium rhizogenes. Turnip hairy root was highly susceptible to P. brassicae; infection rates were high and large galls formed. In contrast, the rates of root hair infection and gall formation on intact
Brassica plants did not differ significantly from the control. To induce resting spore formation, turnip hairy roots were incubated
at 15°, 20°, or 25°C after 3 weeks of incubation at 25°C. The number and fresh mass of the galls per hairy root were higher
and formation of resting spores was greatest after a 7-week incubation at 20°C. To subculture P. brassicae using turnip hairy root, turnip hairy roots were reinoculated with resting spores and gall with resting spores then formed
on the hairy roots. In this way, P. brassicae using hairy roots could be subcultured in vitro two or three times on three single-spore isolates of P. brassicae. This is the first report of in vitro subculture of P. brassicae using hairy root.
Available from: Doo Hwan Kim
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ABSTRACT: Cruciferous hairy roots are often used for improving drought adaptability, peroxidase production, andin vitro subculturing ofPlasmodiophora brassicae. For metabolic engineering,Agrobacterium tumefaciens-mediated systems have previously been developed for hairy root production in other plant species. Here, we used therolABC gene binary construct inA. tumefaciens strain GV3101 to establish cultures of Chinese cabbage hairy roots. On both solid and liquid media, therolABC hairy root lines exhibited a wild-type hairy root syndrome in terms of their growth and morphology. This demonstrates that
those three genes are sufficient to induce high-quality hairy roots in Chinese cabbage. Such a system could be useful for
the stable production of secondary metabolites in that species.
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ABSTRACT: The clubroot disease of cruciferous crops is caused by an obligate biotrophic protist, Plasmodiophora brassicae. The disease is characterized by the development of large root galls accompanied by changes in source-sink relations and
the hormonal balance within the plant. Since the disease is difficult to control, it is of high economic interest to understand
the events leading to gall formation. In this review we will give an overview on the current knowledge of changes brought
about in the host root by this obligate biotrophic pathogen. Emphasis will be on the regulation of changes in plant hormone
homeostasis, mainly auxins and cytokinins; the possible role of secondary metabolites, especially indole glucosinolates, in
gall formation and auxin homeostasis will be discussed. Also, results from mutant analysis and microarrays using the model
plant Arabidopsis thaliana are presented.
Available from: Edson Luis Furtado
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