Biocompatibility of FGL peptide self-assembly nanofibers with neural stem cells in vitro
In order to study the biocompatibility of self-assembled FGL peptide nanofibers scaffold with neural stem cells (NSCs), FGL
pepitide-amphiphile (FGL-PA) was synthesized by solid-phase peptide synthesis technique. The diluted hydrochloric acid was
added into FGL-PA solution to reduce the PH value and accordingly induce self-assembly. The morphological features of the
assembled material were studied by transmission electron microscope. NSCs were cultured and added with self-assembled FGL-PA.
CCK-8 kit was used to test its effect on the proliferation of NSCs. The differentiation of NSCs was also tested after FGL-PA
assembled material added. The experimental results showed that FGL-PA could be self-assembled to form a hydrogel. TEM analysis
showed the self-assembled hydrogel was nanofibers with diameter of 10–20 nm and length of hundreds nanometers. FGL-PA with
concentrations of 50,100, or 200 mg/L could promote the proliferation of NSCs, and absorbance of them was increased (P<0.05). The rate of neurons differentiated from NSCs was improved greatly by FGL-PA assembled material compared with control
(P<0.05). The findings suggested that FGL-PA could self-assemble to nanofiber hydrogel, which had good biocompatibility with
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ABSTRACT: Tissue engineering requires an ideal scaffold that will aid in the regeneration of the damaged tissues both structurallyand functionally. Conventionally, polymeric nanofibrous scaffolds have been extensively used due to their structuralsimilarity to the native extracellular matrix. Thus far, top-down approaches like electrospinning and phase separationhave been predominantly used for the nanofiber fabrication. Recently, self-assembling peptide nanofibers (SAPNF) havebeen identified as promising scaffolds for tissue engineering applications. Molecular self-assembly of peptides, which is abottom-up approach has laid foundations for the development of such novel scaffolds. Designer self-assembling peptidesprovide functional support as well as bio-recognition due to the presence of bioactive motifs embedded in them. However,there are certain limitations to both electrospun and SAPNF scaffolds in terms of synthesis, cues presented to the biologicalsystem and applications. Design of composite, hybrid scaffolds by super-positioning possible cues may result in effectivefunctional tissue regeneration at multiple levels.
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