Host range expansion of honey bee Black Queen Cell Virus in the bumble bee, Bombus huntii

Apidologie (Impact Factor: 1.68). 09/2011; 42(5):650-658. DOI: 10.1007/s13592-011-0061-5


Here we provide the first evidence that Black Queen Cell Virus (BQCV), one of the most prevalent honey bee viruses, can cause
an infection in bumble bees, Bombus huntii, and that the BQCV infection could spread to different tissues of bumble bees. The detection of negative strand RNA of BQCV,
an indicator of active virus replication, in the gut of B. huntii suggests that virus particles replicate within the gut and then cross the gut lining to other tissues through hemolymph circulation.
The observation of active replication of the BQCV in the gut, together with the fact that BQCV was more widespread in the
body of field-collected bees than that of lab-reared bees, implies a possible association between the foraging activities
of bumble bees and virus transmission. The fact that bumble bees and honey bees are able to share nectar and pollen resources
in the same field suggests that geographical proximity of two host species could play a role in host range breadth of BQCV.

Keywordshost range–bumble bee–
Bombus huntii
–Black queen cell virus

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Available from: Jilian Li, Apr 17, 2015
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    • "As these new viruses are not only present in honeybees and differences in prevalence and strains can be found, different transmission routes are possible, probably in a network via contaminated flowers (Singh et al., 2010), honeybees (Fürst et al., 2014; Meeus et al., 2011; Peng et al., 2011; Reynaldi et al., 2014), honeybee parasites (de Miranda et al., 2015; Ravoet et al., 2015), bumblebees (McMahon et al., 2015) and other solitary bees (Levitt et al., 2013; Ravoet et al., 2014). Figure captions Fig. 1. "
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    ABSTRACT: Pollinators such as bumblebees (Bombus spp.) are in decline worldwide which poses a threat not only for ecosystem biodiversity but also to human crop production services. One main cause of pollinator decline may be the infection and transmission of diseases including RNA viruses. Recently, new viruses have been discovered in honeybees, but information on the presence of these in wild bumblebees is largely not available. In this study, we investigated the prevalence of new RNA viruses in Bombus species, and can report for the first time Varroa destructor Macula-like virus (VdMLV) and Lake Sinai virus (LSV) infection in multiple wild bumblebee hosts of Bombus pascuorum, Bombus lapidarius and Bombus pratorum. We sampled in 4 locations in Flanders, Belgium. Besides, we confirmed Slow bee paralysis virus (SBPV) in wild bumblebees, but no positive samples were obtained for Big Sioux river virus (BSRV). Secondly, we screened for the influence of apiaries on the prevalence of these viruses. Our results indicated a location effect for the prevalence of VdMLV in Bombus species, with a higher prevalence in the proximity of honeybee apiaries mainly observed in one location. For LSV, the prevalence was not different in the proximity or at a 1.5 km-distance of apiaries, but we reported a different isolate with similarities to LSV-2 and "LSV-clade A" as described by Ravoet et al. (2015), which was detected both in Apis mellifera and Bombus species. In general, our results indicate the existence of a disease pool of new viruses that seems to be associated to a broad range of Apoidae hosts, including multiple Bombus species.
    Full-text · Article · Dec 2015 · Journal of Invertebrate Pathology
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    • "Other than detecting honeybee pathogens in 110 other insects, and thus establishing possible transmission routes 111 (e.g. (Evison et al., 2012; Li et al., 2011; Peng et al., 2011; Singh 112 et al., 2010), there has been little research as to whether these 113 viruses are actually infectious or, more importantly, cause damage 114 to species other than honeybees. The only recorded exceptions so 115 far are the association of DWV with wing deformities found natu- 116 rally in both wild and commercially reared bumblebees (Genersch 117 et al., 2006), the reduced survival of bumblebees orally inoculated 118 with DWV (Fürst et al., 2014) and the rapid mortality of bumble- 119 bees injected with low doses of Israeli acute paralysis virus (IAPV; "
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    ABSTRACT: Israeli acute paralysis virus (IAPV) together with Acute bee paralysis virus (ABPV) and Kashmir bee virus (KBV) constitute a complex of closely related dicistroviruses. They are infamous for their high mortality after injection in honeybees. These viruses have also been reported in non-Apis hymenopteran pollinators such as bumblebees, which got infected with IAPV when placed in the same greenhouse with IAPV infected honeybee hives. Here we orally infected Bombus terrestris workers with different doses of either IAPV or KBV viral particles. The success of the infection was established by analysis of the bumblebees after the impact studies: 50 days after infection. Doses of 0.5 x 10(7) and 1 x 10(7) virus particles per bee were infectious over this period, for IAPV and KBV respectively, while a dose of 0.5 x 10(6) IAPV particles per bee was not infectious. The impact of virus infection was studied in micro-colonies consisting of 5 bumblebees, one of which becomes a pseudo-queen which proceeds to lay unfertilized (drone) eggs. The impact parameters studied were: the establishment of a laying pseudo-queen, the timing of egg-laying, the number of drones produced, the weight of these drones and worker mortality. In this setup KBV infection resulted in a significant slower colony startup and offspring production, while only the latter can be reported for IAPV. Neither virus increased worker mortality, at the oral doses used. We recommend further studies on how these viruses transmit between different pollinator species. It is also vital to understand how viral prevalence can affect wild bee populations because disturbance of the natural host-virus association may deteriorate the already critically endangered status of many bumblebee species.
    Full-text · Article · Jul 2014 · Journal of Invertebrate Pathology
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    • "In a previous study we reported the detection of deformed wing virus (DWV), black queen cell virus (BQCV), Israeli acute paralysis virus (IAPV), Kashmir bee virus (KBV), and sacbrood virus (SBV) in 11 non-Apis hymenopteran species and in pollen pellets from forager bees (Singh et al., 2010). Other studies have also identified DWV and BQCV infections in some species of bumble bees, including Bombus terrestris, Bombus pascuorum, and Bombus huntii (Genersch et al., 2006; Li et al., 2011; Meeus et al., 2010; Morkeski and Averill, 2010; Peng et al., 2011). Other viruses normally found in A. mellifera, including acute bee paralysis virus (ABPV) and KBV, have also been identified in bumble bees (Anderson, 1991; Bailey and Gibbs, 1964). "
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    ABSTRACT: There are a number of RNA virus pathogens that represent a serious threat to the health of managed honey bees (Apis mellifera). That some of these viruses are also found in the broader pollinator community suggests the wider environmental spread of these viruses, with the potential for a broader impact on ecosystems. Studies on the ecology and evolution of these viruses in the arthropod community as a whole may therefore provide important insights into these potential impacts. We examined managed A. mellifera colonies, nearby non-Apis hymenopteran pollinators, and other associated arthropods for the presence of five commonly occurring picorna-like RNA viruses of honey bees-black queen cell virus, deformed wing virus, Israeli acute paralysis virus, Kashmir bee virus and sacbrood virus. Notably, we observed their presence in several arthropod species. Additionally, detection of negative-strand RNA using strand-specific RT-PCR assays for deformed wing virus and Israeli acute paralysis virus suggests active replication of deformed wing virus in at least six non-Apis species and active replication of Israeli acute paralysis virus in one non-Apis species. Phylogenetic analysis of deformed wing virus also revealed that this virus is freely disseminating across the species sampled in this study. In sum, our study indicates that these viruses are not specific to the pollinator community and that other arthropod species have the potential to be involved in disease transmission in pollinator populations.
    Full-text · Article · Jul 2013 · Virus Research
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