Optimization of Lipase Production using Differential Evolution

Biotechnology and Bioprocess Engineering (Impact Factor: 1.11). 04/2010; 15(2):254-260. DOI: 10.1007/s12257-009-0163-3


Differential Evolution (DE) coupled with Response Surface Methodology (RSM) has been used for the optimization of extracellular
lipolytic enzyme production by Rhizopus oryzae NRRL 3562 through sold state fermentation. The input space of the experimentally validated RSM-model was optimized using
a novel Differential Evolution approach (DE), which works based on the natural selection and survival of the fittest concepts
of the biological world. The maximum lipase activity of 96.52 U/gds was observed with the DE stated optimum values of 35.59°C,
1.50, 5.28, and 4.83 days for temperature, liquid to solid ratio, pH, and incubation time respectively. The optimal levels
of control parameters namely number of population, generations, crossover operator, and mutation constant were equal to 20,
50, 0.6, and 0.20, respectively. The developed model and its optimization are generic in nature and thus appear to be useful
for the design and scale-up of the extracellular lipase production by R. oryzae NRRL 3562 through solid state fermentation.

KeywordsOptimization-lipase-solid state fermentation-
Rhizopus oryzae NRRL 3562-Differential Evolution (DE)

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Available from: Vijay Kumar Garlapati
    • "This shows that the optimum pH for the lipase production was around a pH of 8. In another approach of optimization, Garlapati et al. (2010) have used modeling combined with optimization as two vital steps for maximizing the efficacy of SSF. Response surface methodology (RSM), a statistical technique that generates a mathematical model, coupled with differential evolution, which is an optimization technique, has been used. "
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    ABSTRACT: Solid state fermentation (SSF), a process that occurs in the absence or near absence of water, has been used for the production of various high value added products such as enzymes and other organic components. This paper reviews the recent studies reported on the use of SSF for the production of enzymes; lipases, proteases, cellulases, hemicellulases, ligninases, glucoamylases, pectinases and inulinases. The microorganisms used for fermentation are mostly fungi and substrates are waste materials from the agriculture and food industry. This shows the advantages of SSF from an economical and environmental viewpoint. The paper provides an update on several issues, viz. wastes, microorganisms and scale-up and control of the process of fermentation in solid-state.
    No preview · Article · Feb 2015 · Critical Reviews in Environmental Science and Technology
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    • "Lipase was produced using wheat bran as a substrate through solid-state fermentation [18]. Lipase immobilization was carried out using covalent attachment technique using activated silica as immobilization matrix and was explained in our previous work [19] "
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    ABSTRACT: Solvent-free biosynthesis of benzyl acetate through immobilized lipase-mediated transesterification has been modeled and optimized through statistical integrated artificial intelligence approach. A nonlinear response surface model has been successfully developed based on central composite design with transesterification variables, namely, molarity of alcohol, reaction time, temperature, and immobilized lipase amount as input variables and molar conversion (%) as an output variable. Statistical integrated genetic algorithm optimization approach results in an optimized molar conversion of 96.32% with the predicted transesterification variables of 0.47 M alcohol molarity in a reaction time of 13.1 h, at 37.5°C using 13.31 U of immobilized lipase. Immobilized lipase withstands more than 98% relative activity up to 6 recycles and maintains 50% relative activity until 12 recycles. The kinetic constants of benzyl acetate, namely, and were found to be 310 mM and 0.10 mmol h−1 g−1, respectively.
    Full-text · Article · Sep 2013
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    • "Extracellular lipase production by Rhizopus oryzae NRRL 3562 was optimized by response surface methodology (RSM) through SSF using wheat bran. The optimum values of 35.6 • C, 1.5, 5.28, and 4.83 days for temperature, liquid to solid ratio, pH, and incubation time respectively led to a maximum activity of 96.52 U/g (Garlapati and Banerjee, 2010). The effects of twelve medium components on wheat bran for A. niger lipase production were preliminary screened using Plackett–Burman (PB) design and six components (glucose, yeast extract, peptone, NaH 2 PO 4 , KH 2 PO 4 and water content) were optimized, which yielded an activity of 33.03 U/ml (Contesini et al., 2009). "
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    ABSTRACT: Bioconversion of agricultural residues for lipase production as well as other value added products would hold a prominent position in future biotechnologies, mainly because of its eco friendliness and flexibility to both developing and developed countries. Several residues such as straw, bran, oil cakes, among others attract increasing attention as abundant and cheap renewable feedstock. Many researchers considered improvement of substrate composition, physical parameters (temperature, pH, moisture content and particle size), inoculum concentration, and substrate porosity to upgrade and valorize these bioproducts. Several species of fungi, yeast and bacteria have been used in utilizing the agricultural residues through fermentation techniques due to their ability to grow on particle surfaces as sources of carbon and energy, and produce important industrial enzymes including lipases. This review provides an overview of the present status on the utilization of renewable residues in the form of solid- and liquid-state and their characteristics for production of lipases using different microbial systems.
    Full-text · Article · Jan 2012 · Resources Conservation and Recycling
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