Diallel analysis of maize inbred lines for carotenoids and grain yield

Euphytica (Impact Factor: 1.39). 01/2010; 182(3):395-404. DOI: 10.1007/s10681-011-0513-z


Vitamin A deficiency causes xerophthalmia in preschool-aged children worldwide. The objective of this study was to estimate
the genetic parameters that would be useful in selecting parent plants for developing productive hybrids with higher levels
of provitamin A in the maize kernel. A complete 7×7 diallel mating scheme was used to generate 21 single-cross hybrids.
The F1 crosses and check hybrids were evaluated in complete block design across three different Brazilian environments, and carotenoid
content was analyzed by high performance liquid chromatography. General combining ability effects were significant for all
traits except α-carotene This result indicates that the contribution of the additive effect was more important for provitamin
A and other carotenoids and, consequently that there is high chance of improving this trait through recurrent selection methods.
Line 3 produced the highest level of kernel provitamin A among the inbred lines evaluated and also demonstrated the potential
to contribute to the development of genetic materials with a good performance for provitamin A. Inbred lines 1, 6, and 7 showed
a higher concentration of favorable alleles for grain yield, and inbred lines 3 and 6 exhibited a higher concentration of
favorable alleles for β-carotene. Hybrid 1×3 performed well in terms of provitamin A and grain yield and combination 2×3
was the best performer in terms of lutein content.

KeywordsCarotenoids–Provitamin A–Grain yield–Maize–General and specific combining ability

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    • "Based on the clustering pattern of the genotypes and their kernel carotenoid concentrations, potential heterotic combinations can be identified and used in the breeding program to exploit heterosis. It is important to note that the preponderance of additive gene action has been reported for carotenoids (Egesel et al., 2003; Senete et al., 2011). Thus while generating heterotic hybrid combinations; it is advisable that both the parents should have higher concentration of specific carotenoids besides being genetically distant for the exploitation of heterosis for grain yield. "

    Full-text · Article · Jul 2015
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    • "Several authors also suggested the possibility of exploiting heterosis for improving the concentration of carotenoids (Egesel et al., 2003; Senete et al., 2011; Wong et al., 2004). However, Burt et al. (2011b) affirmed that heterosis is rare for carotenoid content, and proposed QTL analysis to explain this phenomenon. "
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    ABSTRACT: Maize (Zea mays L.) is a promising crop for improving the nutritional quality of foods, because of the presence of broad natural variability for the content of carotenoids. One hundred Italian and nine public lines were screened by PCR to identify the alleles of Hydroxylase3 (HYD3) and Lycopeneepsilon cyclase(LCYE) genes, codifying for key enzymes of the carotenoid pathway and associated with high or low β-carotene contents. Nineteen selected genotypes carrying different alleles were grown in 2011 and/or 2012, and used to obtain F1 seeds. HPLC analysis highlighted the richness in zeaxanthin and β-cryptoxanthin of Italian germplasm (mean values 12.63 and 2.88 mg/kg d.m., respectively). The percentage of positive and significant mid parent heterosis (MPH) and best parent heterosis (BPH) values (51% and 29%) indicated the presence of a high heterotic response for carotenoids. The compounds on the same branch of the pathway were found to be positively correlated; on the contrary, no consistent correlation was observed between the different HYD3 alleles and β-carotene content. Finally, total antioxidant capacity (TAC) was also measured. These lines resulted therefore as interesting raw materials for the market of maize-based foods.
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    ABSTRACT: Vitamin A deficiency has become a worldwide problem. Biofortified foods can potentially be an inexpensive, locally adaptable, and long-term solution to dietary-nutrient deficiency. In order to improve the β-carotene content in maize grain by breeding and minimize vitamin A deficiency, a complete diallel cross was designed with eight inbred lines of maize, and 64 combinations were obtained in this study. The experimental combinations were planted in Yunnan and Sichuan provinces, respectively, with a random complete block design. The β-carotene contents in the grains of the experimental materials were analyzed by high-performance liquid chromatography. Among the tested materials, the effect difference of general combining ability of the β-carotene content was significant; however, the effect difference of the special combining ability and the reciprocal effect were not significant. The β-carotene content of maize grain was not influenced significantly by the cross and the reciprocal cross. There was a significant correlation about the β-carotene content in the maize grains between the F1 and their parents. The combinations with high β-carotene content were obviously influenced by the environment, and the mean value of β-carotene content for the experimental materials planted in Ya'an of Sichuan was higher than that planted in Yuanjiang of Yunnan, with the results being significant at the 0.01 level.
    No preview · Article · Jan 2013 · Journal of Integrative Agriculture
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