Improved Ultra-Performance LC Determination of Indapamide in Human Plasma

Chromatographia (Impact Factor: 1.41). 09/2007; 66:119-122. DOI: 10.1365/s10337-007-0300-0


A simple, rapid, sensitive and selective method for the analysis of indapamide in human plasma, utilizing ultra performance
liquid chromatography (UPLC), has been developed and validated to satisfy FDA guidelines for bioanalytical methods. The analyte
and the internal standard, sulfamethazine, were isolated from plasma samples by liquid–liquid extraction with diethyl ether.
Separation was performed with an Acquity C18 column. The gradient composition of mobile phase was composed of acetonitrile
and sodium dihydrogenphosphate buffer (adjusted to pH 3.33 with 85% o-phosphoric acid) at a flow rate of 0.5mLmin−1. The assay exhibited a linear dynamic range of 1–100ngmL−1 for indapamide in human plasma. The limit of quantification (LOQ) was 1ngmL−1. The method was successfully applied to the pharmacokinetic and bioequivalence studies of indapamide formulations.

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