Mini-cuttings: An effective technique for the propagation of Pinus pinaster Ait

New Forests (Impact Factor: 1.83). 05/2011; 41(3):399-412. DOI: 10.1007/s11056-010-9232-x


Pinus pinaster Ait. is one of the main forest tree species planted in Spain, Portugal and France. Due to its high economic relevance, there
is considerable interest in developing techniques for vegetative breeding aimed at mass propagation. In this study we present
a mini-propagation protocol in order to define an efficient method to propagate families or clones of P. pinaster. We carried out three experiments using mini-cuttings of 3–5cm in length with the aim of evaluating the effects of temperature
(4°C vs. 25°C), plant growth regulator (IBA) and shoot age on rooting ability. Percentage of rooted cuttings and morphological
root variables were recorded. The percentage of rooted cuttings per treatment ranged from 68 to 97%. Treatment with IBA significantly
influenced the rooting process at 25°C but not at 4°C. The number of apexes, length, area and volume of roots were all positively
affected by temperature treatment. Shoot age also had a positive effect on rooting capacity of cuttings, with the cuttings
from the youngest shoots (70days after pruning) having higher rooting percentages, ranging from 84.7 to 98.3%. The use of
juvenile material, good environmental conditions and IBA all benefited the rooting of clonal material, resulting in high rooting
capacity. This study presents an innovative propagation protocol for P. pinaster that can be used as a tool in breeding programs.

KeywordsMaritime pine–Vegetative propagation–Rooting–Shoot age–Indolebutyric acid–Temperature

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Available from: Celia Martínez-Alonso
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    • "Despite major advances in clonal technology of eucalypts, production of rejuvenated stock plants with highest rooting potential demands further investigations in each species. Various attempts have been made to increase the rooting rate of the vegetative cuttings by optimizing the environment, hormone applications and physiological aspects of the hedge stock (Wendling et al. 2010; Majada et al. 2011; Trueman et al. 2013). In the recent years, production of planting stock through microcutting technique is widely utilized for commercial production of eucalypts hybrid clones (Rezende et al. 2014). "
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    ABSTRACT: Adventitious rooting, a key step in clonal propagation is affected by tree maturation. Micropropagation followed by microcuttings propagation has the potential to rejuvenate the clones thereby enhancing the rooting potential and minimize intra clonal variation. In this study, 33 superior performing Eucalyptus camaldulensis clones were propagated by rooting of stem cuttings (SCs) and micropropagation. Micropropagated plantlets were used as stock plants for microcutting propagation. Rooting of SCs and micropropagation was carried out with the coppice shoot cuttings and axillary buds respectively, obtained from approximately fourteen year old trees that had undergone one vegetative propagation cycle. The adventitious rooting recorded was significantly higher in micropropagation (24.8–100 %) and microcuttings (43–95 %) than SCs method (9.3–75.5 %). Studies on ontogeny of adventitious rooting showed the emergence of root primordium from the phloem region and root initials were noticed within 5–9 days after auxin treatment. Further, molecular marker analysis showed genetic uniformity except for two ramets, detected using inter simple sequence repeats (ISSR) markers and suitable corrective measures were taken to avoid entry of such plantlets for mass multiplication. This study demonstrates the importance of integration of micropropagation and microcuttings production for rejuvenation and mass multiplication. Although current rejuvenation and root induction treatments favored adventitious rooting, the basic mechanisms involved in rejuvenation and adventitious rooting need to be explored for hassle free industrial rooting process, consequently cost effective propagation.
    Full-text · Article · Dec 2014 · New Forests
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    • "Pinus pinaster Ait. forests within the Mediterranean are of great economic importance (Majada et al., 2010). In Spain, this species occupies nearly 1.7 million ha, 0.6 of which were established through direct planting (Alía et al., 1996), making it the second most important tree species by surface area in Spain (Rodríguez et al., 2008). "
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    ABSTRACT: The goal of this project was to study how fungal communities in Mediterranean forests dominated by Pinus pinaster are influenced by different edaphoclimatic conditions, characterized by three different edaphoclimatic sites composed of siliceous, calcareous, and sandy soils. Sporocarps were collected and identified from nine 100 m 2 permanent plots, three in each site, during the autumn seasons from 2006 to 2012. The data collected were used to assess fungal productivity and diversity. Fungal community composition was mainly correlated with climatic variables, such as precipitation and temperature. Additionally, soil nitrogen and potassium significantly influenced the distribution of species for both saprotrophic and mycorrhizal functional groups. Some fungi such as Lycoperdon perla-tum, Russula torulosa, and species within the genera Galerina and Mycena were adapted to a broad range of ecological conditions. Others were found only in very specific environmental conditions. All species within Macrolepiota were collected in the calcareous soils, whereas Laccaria laccata and L. bicolor were exclusively associated with higher amounts precipitation and nitrogen in the siliceous plots. These findings have ecological implications that are important for managers seeking new options for adding economic value and for sustaining biological diversity in these Mediterranean forests.
    Full-text · Article · Nov 2014 · Forest Ecology and Management
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    • "Juvenile shoots near the base of the tree possess higher adventitious rooting capacity and root vigour (Haines and Walker 1993; Bon et al. 1994; Bitencourt et al. 2009) because rooting capacity is heavily influenced by both position in the crown (topophysis) and plant age (cyclophysis) (Haines and Walker 1993; Aderkas and Bonga 2000). The use of juvenile cuttings increases rooting capacity and improves morphological variables such as root length, root surface area and root volume in Eucalyptus grandis (Wendling and Xavier 2005; Abu-Abied et al. 2012), Ilex paraguariensis (Wendling et al. 2007), Pinus pinaster (Majada et al. 2011) and Grewia optiva (Husen 2012). Increasing stock plant age decreases the early growth, root weight and root-shoot ratio of P. radiata cuttings (McGranahan et al. 1999). "
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    ABSTRACT: Progression from the juvenile to mature phase in woody plants is accompanied by changes in characteristics as diverse as adventitious rooting capacity, leaf morphology, canopy architecture, wood anatomy and reproductive development. Many concepts of phase change, the intensity and duration of changes that occur during the phase transition, and the practical consequences of plant maturation for growth and development, are poorly understood. Little is known about the physiological and environmental control of maturation in woody plants compared with herbaceous plants, and reliable markers of phase state have only been developed for a few species, mainly conifers. Understanding the mechanisms and forms of phase change is a prerequisite for achieving maturation or rejuvenation for applications such as seed production or clonal propagation. This review describes concepts, terminology and consequences of phase change, combining theoretical and practical aspects of tree maturation that relate to clonal forestry.
    Full-text · Article · Mar 2014 · New Forests
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