High frequency plant regeneration from the mature seeds of Garcinia indica

ArticleinBiologia Plantarum 55(3):554-558 · September 2011with31 Reads
Impact Factor: 1.85 · DOI: 10.1007/s10535-011-0124-5

    Abstract

    A high frequency plant regeneration system was developed for the production of high yielding elite clones of Garcinia indica via direct organogenesis. A maximum number of 86.2 shoot buds per explant were induced from the mature seed segments cultured
    on Woody plant medium (WPM) supplemented with 2.0 mg dm−3 N6-benzyladenine and 1 mg dm−3 indole-3-acetic acid. Rooting was achieved on half-strength WPM medium supplemented with 3 mg dm−3 indole-3-butyric acid. Rooted plantlets were acclimatized and transferred to greenhouse for further growth. The highest survival
    rate of 95 % was recorded using a mixture of garden soil and sand. Histological studies clearly revealed multiple shoot formation
    from mature seed segments.

    Additional key wordsdirect organogenesis–kokum elite clones–mass multiplication–multiple shoots