Enantiomeric Separation of Nefopam Hydrochloride by Affinity Electrokinetic Chromatography Using Chondroitin Sulfate A as Chiral Selector and Its Chiral Recognition Mechanism

ArticleinChromatographia 72(5):489-493 · September 2010with12 Reads
Impact Factor: 1.41 · DOI: 10.1365/s10337-010-1670-2


    In this study, a new approach to the enantioseparation of nefopam hydrochloride by means of affinity electrokinetic chromatography
    (AEKC) with chondroitin sulfate A belonging to linear ionic polysaccharides has been developed. The difference in the antinociceptive
    activity of the enantiomers of nefopam was demonstrated in some studies, and the method established in this paper allowed
    complete separation of nefopam. Especially, there are no reports concerned with the enantioselective separation of nefopam
    using chondroitin sulfate A as chiral selectors in CE. During the course of this work, both migration time and enantioseparation
    of nefopam were influenced by several parameters such as pH of the BGE, selector concentration, capillary temperature and
    applied voltage. Consequently, these parameters were systematically optimized in order to obtain the optimum enantioseparation
    of nefopam. Moreover, comparison of the influences of the studied parameters was further investigated using univariate analysis
    of variance as a calculation method by Statistical Product and Service Solutions (SPSS) in this paper. Finally, a mechanism
    of enantiorecognition in AEKC towards the enantiomers of nefopam with chondroitin sulfate A was described.

    KeywordsAffinity electrokinetic chromatography-Enantiomeric separation-Polysaccharides-Nefopam hydrochloride-Chondroitin sulfate A