Determination of 2-amino-1-methyl-6-phenylimidazo [4,5-b]pyridine (PhIP) in human hair by solid-phase extraction and gas-chromatography-mass spectrometry

ArticleinChromatographia 52(7):499-504 · April 2012with8 Reads
Impact Factor: 1.41 · DOI: 10.1007/BF02535726

    Abstract

    A method for the determination of the food mutagen 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in human hair has been developed and validated. Hair samples (200 mg) were dissolved in NaOH (1 M) and PhIP
    was isolated by successive solid-phase extraction on a polystyrene-divinylbenzene column and on a silica-based mixed-mode
    column with C8 and-SO3
    − functional groups. Quantification was performed by gas chromatography-electron-impact ionization high-resolution mass spectrometry
    in selected-ion-monitoring mode. The method was validated for determination of PhIP in the concentration range 0.5–25 ng g−1 hair with [2H3]PhIP as internal standard. The limit of quantification was 0.26 ng g−1 hair. Within-day and between-day precision were in the ranges 1–27% and 2–15% relative standard deviation, respectively.
    The hair sample used for method validation was found to contain 0.26 ng PhIP g−1 hair.