Article

Inhibitory Effects of Blueberry Root Methanolic Extract on Degranulation in KU812F Cells

Food science and biotechnology (Impact Factor: 0.65). 10/2010; 19(5):1185-1189. DOI: 10.1007/s10068-010-0169-9

ABSTRACT

The effect of blueberry (Vaccinium angustifolium) root methanolic extract (BRM) on the A23187 plus phorbol myristate acetate (PMA)-induced degranulation in human basophilic
KU812F cells was investigated. The total phenolic content (TPC) was 170±1.9 mg gallic acid equivalents (GAE)/g BRM. BRM reduced
levels of histamine and β-hexosaminidase released from stimulated KU812F cells. Intracellular calcium concentration ([Ca2+]i) were determined by spectrofluorometric analysis using Fura 2-AM was found to be reduced by BRM with dose-dependent manner.
Moreover, Western blot analysis revealed that protein kinase C (PKC) translocation was inhibited by BRM with dose-dependent
manner. These results indicated that BRM inhibited histamine and β-hexosaminidase through the suppression of Ca2+ influx and PKC translocation. Therefore, we suggest that BRM is potent inhibitor of degranulation in mast cells and basophils,
and may be useful in preventing the allergic reactions.

Keywords
Vaccinium angustifolium
-degranulation-calcium influx-protein kinase C

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    ABSTRACT: Cross-linking of FcεRI with multivalent allergen and specific IgE on mast cells and initiates activation of signal cascades that lead to IgE-mediated allergic responses. Kaempferol is a phenolic compound contained in natural substances including the stamens of flavonoid-rich Nelumbo nucifera stamens. In this study, the effects of kaempferol on allergic reaction induced by an FcεRI α chain antibody were investigated. Western blot analysis revealed that the protein expression of FcεRI and the phosphorylation of Syk, Lyn, and extracellular signal-regulated kinases (ERK) 1/2 were dose-dependently inhibited by kaempferol. β-Hexosaminidase was reduced to 97.9, 89.1, 75.1, and 55.8% when treated with 5, 10, 20, and 40 μM kaempferol, respectively. From the results, the negative regulation of basophil activation with kaempferol via the suppression of FcεRI expression and degranulation may be suggested to be through inhibition of Lyn and Syk, and ERK 1/2 activation. © 2015, The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht.
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