Comparative study on the determination of serum thyroid hormones by two methods of immunoassay in broiler breeder poultry
Variations in blood thyroid hormone concentrations give an indirect indication of changes in thyroid gland activity; therefore,
selecting a suitable method to determine these hormones is crucial. The aim of this study was to compare serum thyroid hormone
concentrations (i.e. T4, T3, fT4 and fT3) using chemiluminescence immunoassay (CLIA) and enzyme-linked immunosorbent assay (ELISA) methods in broiler breeders. This
study was carried out on 30 blood samples from randomly selected 45-week-old broiler breeders. Blood samples were collected
in summer from the wing vein of birds by using disposable syringes. The separated serum was analysed to determine thyroxine
(T4), triiodothyronine (T3), free thyroxine (fT4) and free triiodothyronine (fT3) concentrations using the mentioned methods. Data were processed in excel and statistical analysis was carried out using
SPSS/ver17 software. Our data from this study indicates there was a significant difference in the T4 (P < 0.0001), fT4 (P < 0.01) and fT3 (P < 0.05) concentrations between the two methods and that the levels of these hormones were higher when using the CLIA method,
except for fT3. The linear regression analysis of these hormones showed that the CLIA and ELISA results were significantly correlated only
for fT4 (P < 0.0001, r = 0.798) concentration. In conclusion, determination of the thyroid hormones using the CLIA method had an acceptable performance
of higher sensitivity and precision and was more appropriate than the ELISA method for clinical application and investigation
KeywordsThyroid hormones–Chemiluminescence immunoassay–ELISA–Poultry
Available from: Rahim Beheshti
[Show abstract] [Hide abstract]
ABSTRACT: The aim of this study was to determine serum free-triiodothyronine (fT 3) level and its correlation between the CLIA and ECLIA methods in cow. Blood samples were collected from the jugular vein of 25 clinically healthy animals aged from 2 to 4 years. This study was performed in August. The separated sera were analyzed to measurement of fT 3 concentration using the CLIA and ECLIA methods. Our results indicate that there was no a significant difference between two method concerning the fT 3 concentration in cow, but its mean was higher in CLIA method. The regression analysis revealed a significant correlation between two methods: (p = 0.022, r = 0.460 for fT 3). It was concluded that the CLIA and ECLIA methods can be used as an alternative to the previous methods for assaying the fT 3 concentration in veterinary diagnostic laboratories and exhibited an acceptable of sensivity and precision for the serum fT 3 determination in cow.
Available from: Behrad Eshratkhah
[Show abstract] [Hide abstract]
ABSTRACT: The thyroid is among the most important endocrine glands that its hormones activity has different effects on metabolism of various components in animal tissues. This study was carried out to determine the relationship between the changes of serum thyroid hormones (T 3, T 4, fT 3 fT 4) concentrations and their percentages, total protein, albumin, cholesterol, glucose concentrations and also albumin: globulin (A:G) T 3:T 4, fT 3: fT 4 ratios in different ages of layer breeders. A total of 49 layer breeder was randomly selected and then the birds were divided into seven age groups (22, 24, 26, 28, 30, 32 and 34 weeks). Blood samples were collected from the wing vein of the birds. The serum was analyzed to determine thyroxine (T 4), triiodothyronine (T 3), free thyroxine (fT 4), free triiodothyronine (fT 3) and total protein, globulin, albumin, glucose and cholesterol concentrations and also above mentioned ratios. The concentrations of thyroid hormones were determined by electrochemiluminescence method and other parameters were measured by photometric method. In the current study, there was a significant difference between age groups concerning the serum total protein (P<0.001), albumin (P<0.01), globulin (P<0.001), cholesterol (P<0.01) concentrations, T 3:T 4 (P<0.05) and fT 3: fT 4 (P<0.001) ratios and also concerning T 4 (P<0.01), T 3 (P<0.001), fT 3 (P<0.01) concentrations and their percentages (P<0.05). Our data from the layer breeder demonstrated that the higher concentration of total protein, globulin, T 3 and its percentage were observed in group VII and an increase in age results in significant increase in the plasma total protein (P<0.001, r = 0.568), globulin (P<0.001, r 0.616) and T 3 (P<0.001, r = 0.534) concentration also its percentage (P<0.01, r = 0.388), T 3:T 4 (P<0.01, r = -0.426) and fT 3:fT 4 ratios while results in significant decrease in T 4 percentage (P<0.01, r = -0.379). It was concluded that T 3 was metabolically more active compared to the other THs in layer breeder; also, age and related physiological changes had a significant effect on total protein, albumin, globulin and all the THs concentrations and also their percentages, except to fT 4. Furthermore, the determination of serum T 3:T 4 ratio was especially a good indication for analysis the data about the hormonal and blood biochemical metabolites changes and relationship between them compared to calculation of THs percentages in layer breeder.
Available from: Seyed Shamsian
[Show abstract] [Hide abstract]
ABSTRACT: Various methods have been used to determine thyroid-stimulating hormone (TSH) and thyroid hormone concentrations in medical diagnostic laboratories. Selection of a suitable method for diagnosis and monitoring thyroid disorders is necessary. The study aimed to compare chemiluminescent assay (CLA), radioimmunoassay (RIA), and enzyme-linked immunosorbent assay (ELISA) methods for the determination of thyroid hormone levels in human serum. Blood samples were taken from 137 patients with thyroid disease and 58 healthy subjects. The sera were analyzed simultaneously to determine the concentration of TSH, thyroxine, and triiodothyronine by CLA, RIA, and ELISA methods. Significant correlation ranges from 0.663 to 0.876 were found between the methods. CLA was the most sensitive method (100 %), but RIA was the most specific method (100 %) according to the clinical symptoms. RIA was the most specific method for the diagnosis of the thyroid diseases, and CLA assay was the most sensitive method in the detection of thyroid diseases except for measuring T4 concentration in hypothyroidism. RIA showed the highest specificity when it comes to the diagnosis of hypothyroidism. RIA had the highest sensitivity, and CLA showed the highest specificity for all three tests in euthyroid group. Each of the methods showed good sensitivity, specificity, and accuracy. ELISA was found to be suitable for the initial screening of the thyroid disorders. The specificity and sensitivity of CLA and RIA were equally high, proposing them as valuable methods for monitoring patients. However, apart from the hazardous effects of ionizing radiation for environment, RIA is the most reliable method for the quantification of thyroid hormones.
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.