Chemiluminescence detection with separation techniques for bioanalytical applications

Bioanalytical Reviews 12/2009; 1(1):25-34. DOI: 10.1007/s12566-009-0002-1


Chemiluminescence detection is known to be a sensitive, selective, and versatile method that can be used in combination with
separation techniques such as high-performance liquid chromatography, capillary electrophoresis, and chip electrophoresis.
This article reviews the bioanalytical applications of a combination of chemiluminescence detection and separation techniques
published in the literature between 1999 and 2008. Luminol chemiluminescene, peroxyoxalate chemiluminescence, and electrochemiluminescence
have been mainly used for bioanalytical application. In this paper, only the applications of the method for the analysis of
biosamples, serum, plasma, urine, and tissue samples are discussed.

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    ABSTRACT: We are presenting the first method for identification and quantification of antibiotic derivatives in honey samples using regenerable antigen microarrays in combination with an automated flow injection system. The scheme is based on an indirect competitive immunoassay format using monoclonal antibodies bound to the surface of the microarray. The surface of glass slides was coated with epoxy-activated poly(ethylene glycol) and enables direct immobilization of the antibiotic derivatives. The antigen/antibody interaction on the surface of the chip can be detected by chemiluminescence (CL) read-out via CCD camera. The method allows for fast analysis of the four analytes simultaneously and without purification or extraction. An effective data evaluation method also was developed to warrant unambiguous identification of the spots and to establish grey levels of CL intensities. The software developed enables fast and automated processing of the CL images. Dose–response curves were obtained for the derivatives of enrofloxacin, sulfadiazine, sulfamethazine and streptomycin. Spiking experiments revealed adequate recoveries within the dynamic ranges of the calibration curves of enrofloxacin (92% ± 6%), sulfamethazine (130% ± 21%), sulfadiazine (89% ± 20%) and streptomycin (93% ± 4%). Figure Flow-scheme of the chemiluminescence multianalyte chip immunoassay for the determination of antibiotic residues in honey
    Full-text · Article · Apr 2011 · Microchimica Acta
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    ABSTRACT: The thermal decomposition of kaolinite was studied by differential thermogravimetry (DTG) technique under non-isothermal conditions. Samples of industrially treated (washed) kaolin with high content of the medium ordered kaolinite were calcined using a heating rate from 1 to 40 K min− 1. The apparent activation energy and frequency factor for the dehydroxylation of kaolinite was evaluated by Kissinger method as 195 ± 2 kJ × mol− 1 and (8.58 ± 0.33) × 1014 s− 1, respectively. Avrami exponent of the process was estimated using Kissinger empirical kinetic models and Carne equation.Graphical AbstractThe thermal decomposition of kaolinite was studied by DTG technique under non-isothermal conditions using heating rate from 1 to 40 K min− 1. The apparent activation energy and pre-exponential factor of process are 195 kJ × mol− 1 and 8.58 × 1014 s− 1, respectively. The value of Avrami exponent was estimated using Kissinger empirical kinetic models and Carne equation.Research Highlights► The dehydroxylation of kaolinite was studied by DTG under non-isothermal conditions. ► Avrami exponent was estimated using Kissinger kinetic models and Carne equation. ► The apparent activation energy was evaluated by Kissinger method as 195 ± 2 kJ × mol− 1. ► Apparent kinetic exponent corresponds to the diffusion controlled growth.
    Full-text · Article · Mar 2011 · Powder Technology
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    ABSTRACT: Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous environmental pollutants, which can cause cancer in humans. The maximum tolerable limit of benzo[a]pyrene (B[a]P) in drinking water was set to 10 ng/L by the European Commission (Council Directive 98/83/EC), because of its highly carcinogenic and mutagenic effect on humans. In the present investigation, mice were immunized with B[a]P-bovine serum albumin conjugates and 110 generated hybridoma cell lines screened by different techniques to identify clones that produce anti-B[a]P antibodies. Subsequently, a new automated flow-through biochip noncompetitive direct chemiluminescence immunoassay (CLEIA) was compared with conventional indirect and direct enzyme-linked immunosorbent assays (ELISAs). It was demonstrated that the microchip-based screening method compared to ELISA was fast and very sensitive with use of only nanoliter volumes of supernatant. Forty clones could be evaluated in less than 5 min. Six high affinity monoclonal antibodies with different cross-reactivities (CR) for individual PAHs were identified by the chip-based assay and indirect microtiter plate ELISA. In comparison, the direct ELISA in the microtiter plate failed to identify three of these clones. The four antibodies with the highest affinity had half maximum inhibitory concentrations (IC(50) values) between 0.31 and 0.92 μg/L for B[a]P. Affinity constants of these four antibodies were determined by surface plasmon resonance using a water soluble B[a]P-peptide. The observed CR pattern of the four monoclonal antibodies for 16 tested PAHs was quite different. Only one specific antibody for B[a]P was observed, while others were more suitable for class-specific PAH determination.
    Full-text · Article · Jun 2011 · Journal of immunological methods
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