Stimulation of Angiogenesis in Rat Ischemic Limb by Intramuscular Implantation of Mononuclear Fraction Cells from Autologous Bone Marrow

Bulletin of Experimental Biology and Medicine (Impact Factor: 0.36). 02/2011; 150(4):530-534. DOI: 10.1007/s10517-011-1183-5


We studied angiogenic effects of two variants of indirect revascularization (transplantation of mononuclear fraction cells
from autologous bone marrow alone or in combination with laser tunneling) after modeled ischemia of the lower limbs in rats.
Doppler sonography and immunofl uorescence studies showed that intramuscular implantation of isolated fraction of autologous
bone marrow mononuclear cells in combination with laser tunneling of the muscles is most effective and can be recommended
as a method of angiogenesis stimulation in nonreconstructable distal vascular pathology of the extremities.

Key Wordsischemia of extremities–revascularization–bone marrow mononuclear fraction–laser tunneling of muscles–immunofluorescence

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    ABSTRACT: The purpose of this study was to assess whether autologous uncultured bone marrow-derived mononuclear cells (BMMNCs) combined with modified cannulated screw would accelerate the healing of canine femoral neck fracture. BMMNCs were encapsulated within fibrin glue (FG) and implanted into the fractured femoral neck via modified cannulated screw in experiment group, and the control group was treated by modified cannulated screw. Gross observation, radiological examination, histological analysis, and blood vessel microdensity counting were used to compare bone healing of each group at 1, 2, and 3 months. FG was confirmed as an ideal cell-delivery vehicle for BMMNCs proliferation and differentiation in vitro testing. In vivo animal testing, faster new bone formation and fracture healing were confirmed by gross observation, radiological examination, histological analysis in experimental group than in control group at all times points. The blood vessel microdensity counting increased gradually both in the experimental group and control group, but was more obviously in experimental group at 3 months (p < 0.01). These data suggest that autologous BMMNCs combined with modified cannulated screw treatment is an effective therapy for femoral neck fracture and thus, may be an option for clinical applications. © 2013 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 9999:1-6, 2013.
    No preview · Article · Aug 2013 · Journal of Orthopaedic Research