Determination of inorganic arsenic species As(III) and As(V) by high performance liquid chromatography with hydride generation atomic absorption spectrometry detection

Department of Water and Soil Analysis, Adam Mickiewicz University, Posen, Greater Poland Voivodeship, Poland
Central European Journal of Chemistry (Impact Factor: 1.33). 01/2004; 2(1):82-90. DOI: 10.2478/BF02476185


The paper presents the principles and advantages of a technique combining high performance liquid chromatography and hydride
generation atomic absorption spectrometry (HPLC-HGAAS) applied to speciation analysis of inorganic species of arsenic As(III)
and As(V) in ground water samples. With separation of the arsenic species on an ion-exchange column in the chromatographic
system and their detection by the hydride generation atomic absorption spectrometry, the separation of the analytical signals
of the arsenic species was excellent at the limits of determination of 1.5 ng/ml As(III) and 2.2 ng/ml As(V) and RSD of 4.3%
and 7.8% for the concentration of 25 ng/ml. The hyphenated technique has been applied for determination of arsenic in polluted
ground water in the course of the study on migration of micropollutants. For total arsenic concentration two independent methods:
HGICP-OES and HGAAS were used for comparison of results of real samples analysis.

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