Proteomic Analysis of Salt Stress Responses in Rice Shoot

College of Life Sciences, Agriculture University of Hebei, Baoding, 071001 People’s Republic of China
Journal of Plant Biology (Impact Factor: 1.21). 12/2011; 54(6):384-395. DOI: 10.1007/s12374-011-9173-8


To gain a better understanding of the mechanism of rice (Oryza sativa L.) in response to salt stress, we performed a proteomics analysis of rice in response to 250mM NaCl treatment using shoots
of 3-day-old nascent seedlings. The changes of protein patterns were monitored with two-dimensional gel electrophoresis. Of
57 protein spots showing changes in abundance in response to salt stress, 52 were identified by matrix-assisted laser desorption/ionization
time-of-flight mass spectrometry. The identified proteins were classified into eight functional categories. Several novel
salt stress-responsive proteins, including protein synthesis inhibitor I, photosystem II stability/assembly factor HCF136,
trigger factor-like protein and cycloartenol-C24-methyltransferase are upregulated upon salt stress. In order to figure out
the different and similar molecular mechanism among salt and other stresses, regulation of some salt responsive proteins under
other abiotic stress (cold and dehydration) and abscisic acid application was also analyzed. The possible molecular mechanism
of rice seedlings in response to salinity and other stresses were discussed.

KeywordsMass spectrometry–
Oryza sativa
–Proteomics–Salt stress–Two-dimensional gel electrophoresis

Download full-text


Available from: Yu Liang
  • Source
    • "Once lipids are peroxidized, the cells are damaged because the membrane loses fluidity and becomes leakier [7]. Lipids are reported to be peroxidized by stresses, and lipid peroxide is used as a stress-associated marker for NaCl stress [22] [23], low light [24], chronic ozone exposure [25], and many other biotic and abiotic stresses. There have been a few experiments on lipid peroxidation by acute ozone stress in spinach, but they were conducted under very high ozone concentrations (500 ppb), much higher than could occur in ambient air [26] [27]. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Tropospheric ozone is an air pollutant harmful to plants and animals. Its rapid increase at the ground surface has raised serious concern over damage to the quality and yield of agricultural crops. Reactive oxygen species (ROS) are formed in plant cells when that are exposed to a high concentration of ozone, and the ROS are thought to alter gene expression and result in cellular death. Clarification of the ROS formation will provide us with a better understanding of the physiological responses to and signaling pathways of ozone stress in plants. In this study, we investigated the mechanisms of the ROS formation in rice (Oryza sativa L.), the premier crop in Asia. To determine ROS distribution in rice leaves under acute ozone stress, we analyzed superoxide dismutase (SOD) expression, lipid peroxidation, NADPH oxidase activity, and in vivo H2O2 formation. Interestingly, chloroplastic, peroxisomal and mitochondrial SODs down-regulated their expression levels under ozone stress, whereas cytosolic SODs maintained their expression level. Higher lipid peroxidation occurred after the end of ozone exposure, which suggests lipid peroxidation may not be due to ozone directly, but rather to metabolic changes caused by the ozone exposure. Activity of NADPH oxidase did not show significant change. The in vivo analysis indicated accelerated formation of H2O2 about 24 h after the onset of exposure, which suggests that cellular death occurred around this time.
    Full-text · Article · Jun 2013 · Plant Physiology and Biochemistry
  • Source
    • "Both the thylakoid lumenal 19-kDa protein (spots 184, 191) and HCF136 (spot 271) are crucial for PSII assembly and function of D1 protein (Plücken et al. 2002; Sirpiö et al. 2007). Besides, alteration of HCF136 by by salt (Li et al. 2011), drought (Zhang et al. 2010) and heavy metal (Farinati et al. 2009) also offers it as a candidate for stress-marker. Elevated temperatures has been shown to inhibit Fd-dependent cyclic electron flow around PS I (Bukhov et al. 2005). "
    [Show abstract] [Hide abstract]
    ABSTRACT: Miscanthus sinensis is a promising bioenergy crop; however, its genome is poorly represented in sequence databases. As an initial step in the comprehensive analysis of the M. sinensis proteome, we report a reference 2-DE protein map of the leaf. A total of 316 protein spots were excised from the gels, digested with trypsin and subjected to matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) or MALDI-TOF/TOF MS. Two hundred and thirty-two protein spots were identified, which are involved in a variety of cellular functions through distinct metabolic pathways. Functional annotation of the proteins revealed a nearly complete C3 and C4 cycle, starch and sugar synthesis pathway, glycolysis pathway, a significant portion of the pentose phosphate pathway, and many enzymes involved in secondary metabolism such as flavonoid/isoflavonoid, kaurene, chalcone, sesquiterpene and lignin biosynthesis. Other proteins belong to primary metabolism, transcription, protein synthesis, protein destination/storage, disease/defense, cell growth/division, transportation and signal transduction. To test the applicability of the constructed map, we studied the effect of heat stress on M. sinensis leaf proteome. Twenty-five protein spots were upregulated, five were newly induced and twenty-five spots were downregulated by heat treatment. The differentially accumulated proteins were involved in photosynthesis, energy metabolism, gene transcription, protein kinases and phosphatases, signal transduction, protein synthesis and heat shock responses. C4-specific pyruvate orthophosphate dikinase, Rubisco large subunit, Rubisco activase and some associated proteins were upregulated during heat stress and tend to restore upon recovery. Identification of these proteins provides some important clues regarding the way M. sinensis copes with hot climate. This work represents the first extensive proteomic description of M. sinensis and provides a reference map and heat-responsive candidates for future molecular and physiological studies of this bioenergy crop.
    Full-text · Article · Jun 2013 · Planta
  • [Show abstract] [Hide abstract]
    ABSTRACT: Salinity is a major threat limiting the productivity of crop plants. A clear demand for improving the salinity tolerance of the major crop plants is imposed by the rapidly growing world population. This review summarizes the achievements of proteomic studies to elucidate the response mechanisms of selected model and crop plants to cope with salinity stress. We also aim at identifying research areas which deserve increased attention in future proteome studies, as a pre-requisite to identify novel targets for breeding strategies. Such areas include the impact of plant-microbial communities on the salinity tolerance of crops under field conditions, the importance of hormone signaling in abiotic stress tolerance, and the significance of control mechanisms underlying the observed changes in the proteome patterns. We briefly highlight the impact of novel tools for future proteome studies and we argue for the use of integrated approaches. The evaluation of genetic resources by means of novel automated phenotyping facilities will have a large impact for the application of proteomics especially in combination with metabolomics or transcriptomics. This article is protected by copyright. All rights reserved.
    No preview · Article · Jun 2013 · Proteomics
Show more