Article

Rat serum electrolytes, lipid profile and cardiovascular activity onNauclea latifolia leaf extract administration

Indian Journal of Clinical Biochemistry 07/2005; 20(2):29-34. DOI: 10.1007/BF02867397

ABSTRACT

Aqueous extract of the leaf and root ofNauclea latifolia Sm. (Rubiaceae) is used in Nigerian folk medicine for the treatment of hypertension. This work is carried out to investigate
the effect ofNauclea latifolia leaf extract on lipid profile and cardiovascular activity of rats. Normal and 10% coconut oil fed rats were treated with
the water-soluble fraction of the ethanol extract ofNauclea latifolia leaf for 2 weeks. Forty-eight mature male albino rats of the Wistar strain were divided into two experiments of four groups,
each group having 6 animals. Experiment I animals were treated with the water-soluble fraction of the ethanol extract whilst
experiment II animals were fed 10% coconut oil meal before treatment with the water-soluble fraction of the ethanol extract.
A single oral dose ofNauclea latifolia was 170, 340 and 510 mg/kg body wt/day of the extracts respectively for 2 wks. There was no significant change in the lipid
profile of the experimental animals as compared with the controls. There was about 40% relaxation on contracted thoracic aorta
that was pre-contracted with 2 μM phenylephrine. The viability of the tissue was tested against 10 μM of acetylcholine. There
was no significant (P>0.05) change in Na+ concentration in the serum. However, the K+ concentration in the serum of the experimental animals showed a significant increase. The study shows that ethanol extract
ofNauclea latifolia has vasodilator action on the aorta and that lipid profiles of experimental rats were not affected. Furthermore, the increase
in the K+ may be contributing to the vasodilator effect ofNauclea latifolia.

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    • "Serum was used for further biochemical and flame photometric analysis (Jenway, Bibby Scientific). Serum electrolytes were estimated according to previously described methods (Akpanabiat et al., 2005;Gasparotto et al., 2012). In brief, the instrument was calibrated using separate serial solutions of sodium and potassium standards. "
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    • "The preparation of the 10% virgin coconut oil meal was done on regular demand [9] "
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    • "It has also been used to unravel blood -related functions of chemical compounds including plant extracts (Yakubu et al., 2007). The effects of medicinal plant products on hematological parameters of experimental animals have been performed by several workers (Akpanabiatu et al., 2005; Aboderin and Oyetayo, 2006). These studies have been particularly helpful in assessing the safe use or otherwise of such compounds and plant extracts. "
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