Microfluidic techniques for dynamic single-cell analysis

ArticleinMicrochimica Acta 168(3):177-195 · March 2010with14 Reads
Impact Factor: 3.74 · DOI: 10.1007/s00604-010-0296-2

    Abstract

    Dynamic single-cell analysis is a very important and frontier research field of single-cell analysis. Microfluidic techniques
    have become new and effective tools for precise, high-throughput, automatic analysis of single-cell dynamic process. This
    review aims to give an overview of dynamic single-cell analysis methods based on microfluidic platforms, with emphasis on
    the recent developments of microfluidic devices and its application to real-time dynamic monitoring of the signal molecules
    release from single living cell with temporal and spatial resolution, dynamic gene expression in single cells, the cell death
    dynamic events at the level of a single cell, and direct cell—cell communication between individual cell pairs.

    KeywordsDynamic single-cell analysis-Microfluidic techniques-Real-time dynamic monitoring