Studies on the in vitro and in vivo antiurolithic activity of Holarrhena antidysenterica

Department of Pharmacology, Faculty of Pharmacy, University of Karachi, Karachi, Pakistan, .
Urological Research (Impact Factor: 1.39). 05/2012; 40(6). DOI: 10.1007/s00240-012-0483-1
Source: PubMed


Holarrhena antidysenterica has a traditional use in the treatment of urolithiasis, therefore, its crude extract has been investigated for possible antiurolithic effect. The crude aqueous-methanolic extract of Holarrhena antidysenterica (Ha.Cr) was studied using the in vitro and in vivo methods. In the in vitro experiments, Ha.Cr demonstrated a concentration-dependent (0.25-4 mg/ml) inhibitory effect on the slope of aggregation. It decreased the size of crystals and transformed the calcium oxalate monohydrate (COM) to calcium oxalate dehydrate (COD) crystals, in calcium oxalate metastable solutions. It also showed concentration-dependent antioxidant effect against 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals and lipid peroxidation induced in rat kidney tissue homogenate. Ha.Cr (0.3 mg/ml) reduced (p < 0.05) the cell toxicity and LDH release in renal epithelial cells (MDCK) exposed to oxalate (0.5 mM) and COM (66 μg/cm(2)) crystals. In male Wistar rats, receiving 0.75 % ethylene glycol (EG) for 21 days along with 1 % ammonium chloride (AC) in drinking water, Ha.Cr treatment (30-100 mg/kg) prevented the toxic changes caused by lithogenic agents; EG and AC, like loss of body weight, polyurea, oxaluria, raised serum urea and creatinine levels and crystal deposition in kidneys compared to their respective controls. These data indicate that Holarrhena antidysenterica possesses antiurolithic activity, possibly mediated through the inhibition of CaOx crystal aggregation, antioxidant and renal epithelial cell protective activities and may provide base for designing future studies to establish its efficacy and safety for clinical use.

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Available from: Saeed Khan, Apr 04, 2015
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    • "In spite of substantial progress in the study of physiological manifestation of urolithiasis, its exact mechanism is still not clearly understood (Coe et al., 2010). The recent proposed mechanism of stone formation involves urinary supersaturation, crystal nucleation, precipitation, growth, aggregation of crystals and their retention in renal tubular epithelial cells, including the adhesion or endocytosis of crystals by the renal cells (Aggarwal et al., 2013; Khan et al., 2012a). "

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    • "Therefore, drugs with multiple targets, such as antioxidant, anti-inflammatory and antispasmodic properties are an obvious choice for the development of antiurolithiatic drugs. Medicinal plants possess multiple constituents that work in a synergistic manner with minimum side effects and are accessible to a large population (Khan et al., 2012). It can be postulated that interventions with natural antioxidants could mitigate free radical toxicity, which "
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