Improvement of Yeast−Biofuel Cell Output by Electrode Modifications

Industrial & Engineering Chemistry Research (Impact Factor: 2.59). 06/2010; 50(2):557–564. DOI: 10.1021/ie1000949


In this study, a methodology for electrodeposition of nickel nanostructures on carbon felt was developed on the base of pulse plating technique. Different in size, shape, and distribution, Ni-island nanostructures were deposited varying the potential, current, pulse duration, and cycle reiteration. The biocompatibility and nontoxicity of the newly created materials toward Candida melibiosica yeast cells was proven. The prepared Ni-nanomodified carbon felts were investigated as anodes in a two-chamber mediatorless yeast−biofuel cell. Maximum power density values of 720 and 390 mW/m2 were achieved with the electrodes modified under galvanostatic and potentiostatic conditions, respectively, against 36 mW/m2 for the nonmodified ones. The better biofuel cell performance obtained with the Ni-modified electrodes is assigned to an improved electron transfer.

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Available from: Sofia Babanova
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    • "NiFeP 260 mW/m 2 [33] Ni 720 mW/m 2 [30] H. anomala (physical adsorption) "
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    ABSTRACT: This paper reviews the state-of-the art of the yeast-based biofuel cell research and development. The established extracellular electron transfer (EET) mechanisms in the presence and absence of exogenous mediators are summarized and discussed. The approaches applied for improvement of mediator-less yeast-based biofuel cells performance are also presented. The overview of the literature shows that biofuel cells utilizing yeasts as biocatalysts generate power density in the range of 20 to 2440mW/m(2), which values are comparable with the power achieved when bacteria are used instead. The electrons' origin and the contribution of the glycolysis, fermentation, aerobic respiration, and phosphorylation to the EET are commented. The reported enhanced current generation in aerobic conditions presumes reconsideration of some basic MFC principles. The challenges towards the practical application of the yeast-based biofuel cells are outlined. Copyright © 2015 Elsevier B.V. All rights reserved.
    Full-text · Article · Apr 2015 · Bioelectrochemistry (Amsterdam, Netherlands)
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    • "By improvement of the fuel cell design and use of nanomodified anodes, the highest electrical outputs, achieved up to now with mediatorless yeast-based biofuel cells, were obtained (Hubenova et al., 2011). We have established that C. melibiosica 2491 is able to transfer electrons extracellularly through both mechanisms, direct and indirect, according to the continuance of the cultivation process (batch or semi-batch systems) (Babanova et al., 2011; Hubenova et al., 2011). At the same time, it was reported that at normal cultivation (without polarization ) C. melibiosica 2491 expressed high phytase activity (Georgiev et al., 2013). "
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    ABSTRACT: In this paper, we report for the first time that Candida melibiosica 2491 yeast strain expresses enhanced phytase activity when used as a biocatalyst in biofuel cells. The polarization also results in an increase of the yeast biomass. Higher steady-state electrical outputs, assigned to earlier production of endogenous mediator, were achieved at continuous polarization under constant load. The obtained results prove that C. melibiosica yeast-based biofuel cell could be used for simultaneous electricity generation and phytate bioremediation. In addition, the higher phytase activity obtained by interruptive polarization suggests a new method for increasing the phytase yield from microorganisms. This article is protected by copyright. All rights reserved.
    Full-text · Article · Sep 2014 · Yeast
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    • "In the recent years, the utilization of yeast species such as Saccharomyces сerеvisiae [17] [18] [19] [20], Hansenula anomala [21], Hansenula polymorpha [22], Arxula adeninivorans [23] [24] and Candida melibiosica 2491 [25] [26] as biocatalysts in biofuel cells has been reported. The evolutionary conservativeness of respiration complexes allows only partial comparison between the EET mechanism in prokaryotes and eukaryotes. "
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    ABSTRACT: The influence of mitochondrial electron transport chain inhibitors on the electricity outputs of Candida melibiosica yeast-based biofuel cell was investigated. The addition of 30μM rotenone or antimycin A to the yeast suspension results in a decrease in the current generation, corresponding to 25.7±1.3%, respectively 38.8±1.9% reduction in the electric charge passed through the bioelectrochemical system. The latter percentage coincides with the share of aerobic respiration in the yeast catabolic processes, determined by the decrease of the ethanol production during cultivation in the presence of oxygen compared with that obtained under strict anaerobic conditions. It was established that the presence of both inhibitors leads to almost complete mitochondrial dysfunction, expressed by inactivation of cytochrome c oxidase and NADH:ubiquinone oxidoreductase as well as reduced electrochemical activity of isolated yeast mitochondria. It was also found that methylene blue partially neutralized the rotenone poisoning, probably serving as alternative intracellular electron shuttle for by-passing the complex I blockage. Based on the obtained results, we suppose that electrons generated through the aerobic respiration processes in the mitochondria participate in the extracellular electron transfer from the yeast cells to the biofuel cell anode, which contributes to higher current outputs at aerobic conditions.
    Full-text · Article · Jun 2014 · Bioelectrochemistry (Amsterdam, Netherlands)
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