Isolation of Proteins from Polyacrylamide Gels

ArticleinMethods in molecular biology (Clifton, N.J.) 869:427-31 · January 2012with11 Reads
Impact Factor: 1.29 · DOI: 10.1007/978-1-61779-821-4_35 · Source: PubMed


    Minute amounts of proteins are required for immunization of mice for the development of antibodies including monoclonal antibodies. Here, we describe a rapid procedure for the isolation of proteins from polyacrylamide gels after sodium dodecyl sulfate polyacrylamide gel electrophoresis in sufficient amounts for immunization of animals.