Article

Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry-Based Method for Discrimination between Molecular Types of Cryptococcus neoformans and Cryptococcus gattii

Institute of Hygiene, Università Cattolica del Sacro Cuore, Rome, Italy.
Journal of clinical microbiology (Impact Factor: 3.99). 05/2012; 50(7):2472-6. DOI: 10.1128/JCM.00737-12
Source: PubMed

ABSTRACT

We evaluated the usefulness of matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS)
for Cryptococcus identification at the species and subspecies levels by using an in-house database of 25 reference cryptococcal spectra. Eighty-one
out of the 82 Cryptococcus isolates (72 Cryptococcus neoformans and 10 Cryptococcus gattii) tested were correctly identified with respect to their molecular type designations. We showed that MALDI-TOF MS is a practicable
alternative to conventional mycology or DNA-based methods.

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    • "Bien que limitée par l'absence de kits commerciaux [26] [19], cette dernière avancée, considérable, permet d'orienter rapidement la prise en charge thérapeutique. Outre l'intérêt plus récent pour le typage des souches [3] [21], voire la détermination de leur sensibilité aux antifongiques [29], il semble également important d'insister sur l'apport de la SM à l'identification des champignons filamenteux . Dans l'ensemble, celle-ci représente en effet un progrès incontestable, tant les délais des techniques traditionnelles sont généralement non compatibles avec un diagnostic d'urgence [13]. "

    Full-text · Article · Nov 2014
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    • "Bien que limitée par l'absence de kits commerciaux [26] [19], cette dernière avancée, considérable, permet d'orienter rapidement la prise en charge thérapeutique. Outre l'intérêt plus récent pour le typage des souches [3] [21], voire la détermination de leur sensibilité aux antifongiques [29], il semble également important d'insister sur l'apport de la SM à l'identification des champignons filamenteux . Dans l'ensemble, celle-ci représente en effet un progrès incontestable, tant les délais des techniques traditionnelles sont généralement non compatibles avec un diagnostic d'urgence [13]. "

    Full-text · Article · Nov 2014 · Journal de Mycologie Médicale/Journal of Medical Mycology
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    • "At present, there is a simple biochemical difference to distinguish C. neoformans and C. gattii with the use of a canavanine–bromothymol–glycine (CBG) agar plate assay that allows separation of the two species by the color of colonies. The species can also be distinguished by an array of sequencing techniques and even MALDI-TOF can rapidly separate species (Posteraro et al., 2012). While these techniques can separate species and genotypes, the ability for the clinician to use this information does not necessarily translate into differences in treatment for C. neoformans vs C. gattii or specific genotypes. "
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