Anisotropic light scattering of individual sickle red blood cells

Korea Advanced Institute of Science and Technology, Department of Physics, Daejeon 305-701, Republic of Korea.
Journal of Biomedical Optics (Impact Factor: 2.86). 04/2012; 17(4):040501. DOI: 10.1117/1.JBO.17.4.040501
Source: PubMed


We present the anisotropic light scattering of individual red blood cells (RBCs) from a patient with sickle cell disease (SCD). To measure light scattering spectra along two independent axes of elongated-shaped sickle RBCs with arbitrary orientation, we introduce the anisotropic Fourier transform light scattering (aFTLS) technique and measured both the static and dynamic anisotropic light scattering. We observed strong anisotropy in light scattering patterns of elongated-shaped sickle RBCs along its major axes using static aFTLS. Dynamic aFTLS analysis reveals the significantly altered biophysical properties in individual sickle RBCs. These results provide evidence that effective viscosity and elasticity of sickle RBCs are significantly different from those of the healthy RBCs.

Download full-text


Available from: YongKeun Park
  • Source
    • "Normal mature red cells can be described as round, elastic, non-nucleated, bi-concave and cells have an area of central pallor which covers about one-third of the cell. Normal mature red blood cells have an average diameter of 7.2 microns with a range of 6-9 microns (Kim et al., 2012). "
    [Show abstract] [Hide abstract]
    ABSTRACT: Anaemia is one of the blood diseases that are different forms of blood cells. These shapes indicated the type of disease. It is through the study of 10,000 image from 19 types of anaemia has been drawn 40 different shapes of the natural shape of red blood cells. The interesting findings in this study are included Iron deficiency anaemia (IDA) is the most common cases disease; it could reach to 39.5% out of total cases. The most important frequency shapes in IDA are hypocromic microsite (54-62%), Ellipsoids (12-15%), Normocytes (9-11%) and (target, dear drop) (7-9%). Southeast Asian anaemia (SEA) is most common in Malaysia and might be second place after iron deficiency anaemia. The most common shapes of anaemic erythrocytes are stomatocytes (45-64%), kinzocyte (40-45%) and Basophilic (5-8%). Thalassemia showed about 93% of shape of total abnormal RBCs shaped. The majority of abnormal shapes were the hypocromic microsites (54-67%), target (17-21 %) and basophilic cells (8-15%). Megoblastic showed most common shapes of ovalocytosis (41-45%), spherocytosis (29-33%). Haemolytic anaemia is the fourth type of anaemia was shown the erythrocytes look hyporocromic macrosytis, which showed 39% of total abnormal cells. The majority of abnormal shape showed in the Spherocytis (29-35%), Polychromatophilia (13-16%) and Ovalocytosis (9-15%). Red distribution width (RWD) showed highest in IDA, which caused a wide variation in red cell size.
    Full-text · Article · Jul 2013 · International Journal of Scientific and Engineering Research
  • [Show abstract] [Hide abstract]
    ABSTRACT: We report the characterization of all the relevant biomechanical properties of individual red blood cells with sickle cell disease using non-invasive quantitative phase imaging and spectroscopy techniques with a previously-validated RBC membrane model.
    No preview · Conference Paper · Jan 2012
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: We present a high-speed holographic microscopic technique for quantitative measurement of polarization light-field, referred to as polarization holographic microscopy (PHM). Employing the principle of common-path interferometry, PHM quantitatively measures the spatially resolved Jones matrix components of anisotropic samples with only two consecutive measurements of spatially modulated holograms. We demonstrate the features of PHM with imaging the dynamics of liquid crystal droplets at a video-rate.
    Full-text · Article · Apr 2012 · Optics Express
Show more