KEY WORDS: Point-of-care, metering, debubbling, mixing, miniaturization
Traditional molecular diagnosis usually relies on expensive commercial equipment, devices and large quantity of reagents. Moreover, the whole assay is usually very complicated and time consuming (> 1 day), which is not suitable for point-of-care diagnosis of infectious diseases. Here we present a microfluidics ... [Show full abstract] based handheld cassette, which integrates the following modules and functions: a) on-chip storage reagents and waste; b) metering of sample and reagents; c) blood lysis module, DNA extraction module; d) connection to PCR module and detection module; e) debubbling, mixing of reagents and sample; f) accurate fluid control with programmable valving and pumping modules. The cassette is integrated into a handheld system. The operation of cassette is very simple. A user only needs to add one drop of blood sample to the cassette, and the result will be out within 15 minutes automatically.