Article

Genetic variability evaluation in a Moroccan collection of barley, Hordeum vulgare L., by means of storage proteins and RAPDS

Universidad de León, León, Castille and León, Spain
Genetic Resources and Crop Evolution (Impact Factor: 1.46). 03/2002; 49:619-631. DOI: 10.1023/A:1021228730714

ABSTRACT

The genetic variation existing in a set of barley (Hordeum vulgare L.) landrace samples recently collected in
Morocco was estimated. Two kinds of genetic markers, seed storage proteins (hordeins) and random amplified
polymorphic DNA (RAPD), were used. Only six out of 31 landraces were subjected to RAPD analysis. Both kinds
of markers, RAPD and storage proteins, yielded similar results, showing that the level of variation observed in
Moroccan barley was high: all landraces showed variability; 808 different storage protein patterns (multilocus
associations) were observed among 1897 individuals (2.32 seeds per association, on average) with an average of
43 multilocus associations per accession. In general, genetic variation within accessions was higher than between
accessions. The 100 polymorphic RAPD bands generated by 21 effective primers were able to generate enough
patterns to differentiate between uniform cultivars and even between individuals in variable accessions. One of the
aims of this work was to compare the effectiveness of RAPD versus storage protein techniques in assessing the
variability of genetic resource collections. On average hordeins were more polymorphic than RAPDs: they showed
more alternatives per band on gels and a higher percentage of polymorphic bands, although RAPDs supply a
higher number of bands. Although RAPD is an easy and standard technique, storage protein analysis is technically
easier, cheaper and needs less sophisticated equipment. Thus, when resources are a limiting factor and considering
the cost of consumables and work time, seed storage proteins must be the technique of choice for a first estimation
of genetic variation in plant genetic resource collections.

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Available from: EL Habib Dakir, Jun 24, 2015
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    • "Another important issue is where to find genetic variability. Barley (Hordeum vulgare L.) is one of the main cereal crops worldwide (Dakir et al. 2002). Large germplasm collections of cultivated (Hordeum vulgare subsp. "
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    ABSTRACT: Fifteen barley landraces were collected from different localities in the West-Bank,-Palestine during 2009. A field experiment was conducted at the Faculty of Agriculture-An Najah National University to evaluate several agronomical traits of these landraces in 2010-2011 growing season. Cluster analysis was performed using the complete-linkage method, genotypic coefficient of variation (GCV), phenotypic coefficient of variation (PCV), broad sense heritability (H2), and genetic advance (GA) were calculated for the quantitative traits. Significant diversity was exhibited among the landraces regarding days to 90% heading, 100- grain weight, number of grains per spike, spike length, and awns length. The Cluster analysis showed high genetic diversity among the collected landraces with dissimilarity ranging from 0.26 to 0.75. The fifteen landraces were grouped into four clusters. Genotypic coefficient of variation ranged from 6.1 to 22.9, whereas phenotypic coefficient of variation ranged from 6.6 to 41.8 with maximum phenotypic and genotypic variability observed for number of fertile tellers, number of grains per spike and spike length. Moderate to high heritability (broad sense) estimates (70-87%) were found for most of the characters. The genetic advance was highest for number of grains per spike (39.4%), followed by spike length (37.2%). High positive significant correlations were found among the different studied traits with correlation coefficient ranging from 0.395 to 0.536. The results of this study indicated high genetic diversity among barley landraces in Palestine, which make them potential sources for selection and hybridization programmes. © 2015, Pakistan Agricultural Scientists Forum. All rights reserved.
    Full-text · Article · Oct 2015 · Journal of Animal and Plant Sciences
  • Source
    • "Another important issue is where to find genetic variability. Barley (Hordeum vulgare L.) is one of the main cereal crops worldwide (Dakir et al. 2002). Large germplasm collections of cultivated (Hordeum vulgare subsp. "

    Full-text · Article · Oct 2015 · Journal of Animal and Plant Sciences
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    • "Firstly, there must be significant variation for the character to be improved and secondly, the character must be affected by genetic component. In the previous reports, presence of variation in salt tolerance had been observed in different crops, as for example, in wheat (Akhtar et al., 2003; Bhatti et al., 2004; Ali et al., 2007), maize (Rao & McNeilly, 1999; Khan et al., 2003), sorghum (Azhar & McNeilly, 1987, 2000 & 2001), sunflower (Bhutta et al., 2004), barley (Czembor, 2000; Dakir et al., 2002; Madidi et al., 2004), rice (Ahmad et al.,1990; Shannon et al., 1998; Lee et al., 2003). Cotton (Gossypium hirsutum L.) is an important cash crop grown in the area mostly affected by salinity in the country. "
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    ABSTRACT: The genetic basis of salt (NaCl) tolerance at maturity stage was examined in six accessions of Gossypium hirsutum L. at plant maturity. The six accessions were crossed in all possible combinations. The NaCl tolerance of 30 F 1 hybrids and their six parents were assessed at maturity level in the iron containers by subjecting to constant NaCl treatments i.e., 0, 17.5, and 20 dS/m salinity. Indices of salt tolerance (relative salt tolerance) were analyzed using diallel method. Both additive and dominance effects appeared to be important for the expression of variation under low and high salinity levels. Estimates of narrow sense heritabilities for salt tolerance were remarkable. This suggested that rapid improvement in (NaCl) tolerance in G. hirsutum using high selection pressures in the F 2 population may be made through selection and breeding.
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