Pluronic F108 Coating Decreases the Lung Fibrosis Potential of Multiwall Carbon Nanotubes by Reducing Lysosomal Injury

Division of NanoMedicine, Department of Medicine University of California, Los Angeles, California 90095, United States.
Nano Letters (Impact Factor: 13.59). 04/2012; 12(6):3050-61. DOI: 10.1021/nl300895y
Source: PubMed


We compared the use of bovine serum albumin (BSA) and pluronic F108 (PF108) as dispersants for multiwalled carbon nanotubes (MWCNTs) in terms of tube stability as well as profibrogenic effects in vitro and in vivo. While BSA-dispersed tubes were a potent inducer of pulmonary fibrosis, PF108 coating protected the tubes from damaging the lysosomal membrane and initiating a sequence of cooperative cellular events that play a role in the pathogenesis of pulmonary fibrosis. Our results suggest that PF108 coating could serve as a safer design approach for MWCNTs.

  • Source
    • "Not only they are deemed as receptors necessary to study ecological nanotoxicology (Holden et al., 2012), but they also act as facile test subjects that can be used in miniaturized toxicological screening for rapid hazard identification (Jin et al., 2010; Nel et al., 2013). The conclusions from the bacterial toxicity assay can encourage the development of safer nanomaterial designs (Wang et al., 2012). From a thorough literature review, the interaction between the nanoparticles and the bacterial cell leading to cytotoxicity has been hypothesized to involve two steps. "
    [Show abstract] [Hide abstract]
    ABSTRACT: There is a persistent need to assess the effects of TiO2 nanoparticles on the aquatic ecosystem owing to their increasing usage in consumer products and risk of environmental release. The current study is focused on TiO2 nanoparticle-induced acute toxicity at sub-ppm level (≤1ppm) on the three different freshwater sediment bacterial isolates and their consortium under two different irradiation (visible light and dark) conditions. The consortium of the bacterial isolates was found to be less affected by the exposure to the nanoparticles compared to the individual cells. The oxidative stress contributed considerably towards the cytotoxicity under both light and dark conditions. A statistically significant increase in membrane permeability was noted under the dark conditions as compared to the light conditions. The optical and fluorescence microscopic images showed aggregation and chain formation of the bacterial cells, when exposed to the nanoparticles. The electron microscopic (SEM, TEM) observations suggested considerable damage of cells and bio-uptake of nanoparticles. The EPS (exopolysaccrides) production and biofilm formation were noted to increase in the presence of the nanoparticles.
    Full-text · Article · Sep 2014 · Environmental Research
  • Source
    • "Likewise, there is a need for alternative shielding agents that offer comparable, and sometimes better, efficacy after complexation with the particles. Pluronic surfactants such as F127 and F108 coated on MWNT have shown to afford better protection against nonspecific serum protein adsorption and anti-inflammatory processes.140,145 By effectively dispersing MWNTs, F108 passivates the CNT surface by forming a protective brush-like layer that is able to prevent profibrogenic responses in the macrophages through steric hindrance and, hence, have little cellular uptake. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Carbon nanotubes (CNTs) have recently been in the limelight for their potential role in disease diagnostics and therapeutics, as well as in tissue engineering. Before these medical applications can be realized, there is a need to address issues like opsonization, phagocytosis by macrophages, and sequestration to the liver and spleen for eventual elimination from the body; along with equally important issues such as aqueous solubility, dispersion, biocompatibility, and biofunctionalization. CNTs have not been shown to be able to evade such biological obstacles, which include their nonspecific attachments to cells and other biological components in the bloodstream, before reaching target tissues and cells in vivo. This will eventually determine their longevity in circulation and clearance rate from the body. This review article discusses the current status, challenges, practical strategies, and implementations of coating CNTs with biocompatible and opsonin-resistant moieties, rendering CNTs transparent to opsonins and deceiving the innate immune response to make believe that the CNTs are not foreign. A holistic approach to the development of such "stealth" CNTs is presented, which encompasses not only several biophysicochemical factors that are not limited to surface treatment of CNTs, but also extraneous biological factors such as the protein corona formation that inevitably controls the in vivo fate of the particles. This review also discusses the present and potential applications, along with the future directions, of CNTs and their hybrid-based nanotheranostic agents for multiplex, multimodal molecular imaging and therapy, as well as in other applications, such as drug delivery and tissue engineering.
    Full-text · Article · May 2014 · International Journal of Nanomedicine
  • Source
    • "The cellular fate of nanoparticles (NPs), i.e., their intracellular translocation after cellular uptake and their final destiny, is relevant not only to their cytotoxicity12, but also to NP-based drug delivery34, gene delivery56, long term tracking78, and bio-sensing910. While a large amount of research work has been devoted to studying endocytosis (a major route of NPs' cellular uptake11121314), only limited understanding has been obtained on NPs' cellular fate after they enter the cells. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Cellular fate of nanoparticles is vital to application of nanoparticles to cell imaging, bio-sensing, drug delivery, suppression of drug resistance, gene delivery, and cytotoxicity analysis. However, the current studies on cellular fate of nanoparticles have been controversial due to complications of interplay between many possible factors. By well-controlled experiments, we demonstrated unambiguously that the morphology of nanoparticles independently determined their cellular fate. We found that nanoparticles with sharp shapes, regardless of their surface chemistry, size, or composition, could pierce the membranes of endosomes that carried them into the cells and escape to the cytoplasm, which in turn significantly reduced the cellular excretion rate of the nanoparticles. Such features of sharp-shaped nanoparticles are essential for drug delivery, gene delivery, subcellular targeting, and long-term tracking. This work opens up a controllable, purely geometrical and hence safe, degree of freedom for manipulating nanoparticle-cell interaction, with numerous applications in medicine, bio-imaging, and bio-sensing.
    Full-text · Article · Mar 2014 · Scientific Reports
Show more