Selected heavy metals versus antioxidant parameters in bull seminal plasma - A comparative study

Article (PDF Available)inJournal of Environmental Science and Health Part A Toxic/Hazardous Substances & Environmental Engineering 47(9):1261-6 · July 2012with30 Reads
DOI: 10.1080/10934529.2012.672117 · Source: PubMed
Abstract
To investigate the effects of lead (Pb) and cadmium (Cd) content on basic motility characteristics (motility, progressive motility) and selected antioxidant parameters (total antioxidant status - TAS, superoxide dismutase - SOD, albumin - ALB) in the bovine seminal plasma semen samples were collected from breeding bulls and used in the study. Motility analysis was carried out using the Computer Assisted Sperm Analysis (CASA) system. Subsequently, the samples were centrifuged and fractions of seminal plasma were collected. Pb and Cd concentrations were determined by the voltametric method (ASV), antioxidant parameters were analyzed by UV/VIS spectrophotometry using commercial kits. The analysis showed that the average concentrations of the trace elements were 0.57 ± 0.01 μg/mL for Pb and 0.11 ± 0.01 μg/mL for Cd. The correlation analysis revealed that both heavy metals were negatively correlated with motility (r = -0.777; P < 0.001 for Pb and r = -0.786; P < 0.001 for Cd), progressive motility (r = -0.763; P < 0.001 for Pb and r = -0.792; P < 0.001 for Cd), TAS (r = -0.375; p > 0.05 and r = -0.334; P > 0.05, respectively), SOD (r = -0.746; P < 0.001 and r = -0.537; P < 0.05, respectively) as well as with ALB (r = -0.609; P < 0.01 and r = -0.699; P < 0.001, respectively). Moreover the samples were categorized in three quality groups (Excellent, Good, Medium) according to their motility values. The lowest Pb and Cd concentrations but the best antioxidant characteristics were found in samples of excellent quality, medium quality samples were described by the highest Pb and Cd concentration and the worst antioxidant power. This study demonstrates that Pb and Cd are serious toxic elements, which are able to increase the risk of oxidative stress development and a subsequent decrease of semen quality.
    • Although the principles of the assays may be different, both are based on assessing the capacity of all the hydrophilic as well as lipophilic molecules able to metabolize ROS. Independently of the assay applied, the total antioxidant capacity has been found to be associated with semen quality along with sperm viability and functional activity (Khosrowbeygi et al., 2004; Tvrda et al., 2012; Pahune et al., 2013). Furthermore, positive correlations between the parameters in both seminal fractions (Table 3), as well as similar associations between the spermatozoa motility characteristics and biochemical markers (Table 4) suggest that both assays are suitable for the measurement of the in vivo antioxidant status of semen.
    Full-text · Article · Feb 2016
    • At the same time, the semen characteristics met the criteria established for the Holstein Friesian bovine breed, which is why a possible health impact on the outcomes collected from further examinations was ruled out. The data collected from the CRP, IL-6, TAS and MDA assessments suggest that all the studied characteristics were within the physiological limits and comparable to the results of other authors [6,[18][19][20]. Table 2displays the results of the correlation analysis between the selected motion characteristics, inflammatory, prooxidant and antioxidant parameters quantified in bovine seminal plasma.
    [Show abstract] [Hide abstract] ABSTRACT: Oxidative stress and inflammation are cooperative events involved in male reproductive dysfunction. In the present study, we assessed the associations between the spermatozoa motility, inflammatory factors (C-reactive protein and Interleukin-6), total antioxidant status (TAS) and lipid peroxidation expressed as malondialdehyde (MDA) concentration in the seminal plasma of breeding bulls. 17 semen samples were included in the study. Computer-aided sperm analysis (CASA) system was used to assess the spermatozoa motion characteristics, and seminal plasma was collected for further analyses. Interleukin-6 (IL-6) was quantified using ELISA, while C-reactive protein (CRP) and markers of the oxidative balance were evaluated by UV/VIS spectrophotometry. The correlation analysis revealed significantly positive associations between the sperm motility and TAS (P<0.05), while both parameters were in significantly negative correlations with CRP (P<0.05), IL-6 (P<0.05) and MDA (P<0.01). At the same time, the samples were divided according to the motility characteristics into groups of Excellent (Ex) and Moderate (Mo) quality. CRP, IL-6 as well as MDA concentrations were significantly (P<0.05) higher in the Mo group, while the Ex group exhibited a significantly higher antioxidant capacity (P<0.05). The relationships between the oxidative balance and inflammatory markers detected in our study suggest their intricate involvement in the resulting semen quality.
    Full-text · Article · Jan 2016 · Science of The Total Environment
    • SOD activity was then measured by the inhibition degree of the reaction at 505 nm using the Genesys 10 spectrophotometer (Thermo Fisher Scientific Inc., Waltham, USA). The SOD activity is expressed as U/mg protein (Tvrda et al., 2012). Catalase (CAT) activity was assessed according to Beers and Sizer (1952) by monitoring the decrease of hydrogen peroxide (H2O2) at 240 nm.
    Full-text · Article · Sep 2015
    • Numerous animal and human studies have reported a significant association between reactive oxygen species (ROS) overproduction and various types of male infertil- ity,123456 and it is now widely agreed that oxidative stress (OS) significantly contributes to sperm structural or functional abnormalities including protein deterioration, DNA damage and lipid peroxidation (LPO). [7,8] Oxidative damage to the male germ cell is in turn linked to motility loss, alterations of membrane fusion events such as the acrosome reaction and sperm–oocyte fusion, [9] which may lead to poor fertilization rates or impaired embryonic development.
    [Show abstract] [Hide abstract] ABSTRACT: Resveratrol (RES) is a natural polyphenol and phytoestrogen exhibiting cardioprotective, anticancer, antibacterial and vasorelaxing properties. It is also a powerful reactive oxygen species (ROS) scavenger and chelating agent. This study was designed to determine the efficiency of RES to reverse the ROS-mediated impairment of the motility, viability and intracellular antioxidant profile of bovine spermatozoa. Spermatozoa were washed out of fresh bovine semen, suspended in 2.9% sodium citrate and subjected to RES treatment (5, 10, 25 and 50 μmol L−1) in the presence or absence of a pro-oxidant, i.e., ferrous ascorbate (FeAA; 150 μmol L−1 FeSO4 and 750 μmol L−1 ascorbic acid) during a 6-h in vitro culture. Spermatozoa motion parameters were assessed using the SpermVision computer-aided sperm analysis (CASA) system. Cell viability was examined with the metabolic activity (MTT) assay, and the nitroblue-tetrazolium (NBT) test was applied to quantify the intracellular superoxide formation. Cell lysates were prepared at the end of the in vitro experiments in order to investigate the intracellular activity of superoxide dismutase (SOD), catalase (CAT), as well as the concentrations of glutathione (GSH) and malondialdehyde (MDA). FeAA treatment led to a reduced sperm motility (P P P P P P P P P −1 RES and P −1 RES; P −1 RES proving to be the most effective RES concentration. Our results suggest that RES possesses significant antioxidant properties that may prevent the deleterious effects caused by ROS to spermatozoa, and preserve the fertilization potential of male reproductive cells.
    Full-text · Article · Aug 2015
    • Bovine sperm with 0.23–0.57 μg/ml of Pb have shown a reduced fertilization capacity and an altered prooxidant– antioxidant balance, as evidenced in the decrease in GSH and total antioxidant status (TAS), and the increase in lipid peroxidation measured as malondialdehyde (MDA) levels (Tvrda et al., 2012Tvrda et al., , 2013). Humans with elevated levels of Cd and Pb in sperm have also shown reduced fertility accompanied by higher MDA, protein carbonyls and ROS levels, and lower GSH levels and GSH-s-transferase (GST) activities, suggesting an oxidative damage of these heavy metals on lipids and proteins (Kiziler et al., 2007).
    [Show abstract] [Hide abstract] ABSTRACT: Vertebrates are constantly exposed to a diffuse pollution of heavy metals existing in the environment, but in some cases, the proximity to emission sources like mining activity increases the risk of developing adverse effects of these pollutants. Here we have studied lead (Pb) levels in spermatozoa and testis, and chromatin damage and levels of endogenous antioxidant activity in spermatozoa of red deer (Cervus elaphus) from a Pb mining area (n=37) and a control area (n=26). Deer from the Pb-polluted area showed higher Pb levels in testis parenchyma, epididymal cauda and spermatozoa, lower values of acrosome integrity, higher activity of glutathione peroxidase (GPx) and higher values of DNA fragmentation (X-DFI) and stainability (HDS) in sperm than in the control area. These results indicate that mining pollution can produce damage on chromatin and membrane spermatozoa in wildlife. The study of chromatin fragmentation has not been studied before in spermatozoa of wildlife species, and the sperm chromatin structure assay (SCSA) has been revealed as a successful tool for this purpose in species in which the amount of sperm that can be collected is very limited.
    Full-text · Article · Feb 2015
    • Interestingly, ALB, which is not considered to be a dominant protein of the bovine ejaculate [25], exhibited detectable and important concentrations in both seminal plasma and spermatozoa (Table 2). Moreover, positive relationships between ALB, spermatozoa motility, and antioxidant profile confirms the suggestions of Bourdon and Blache [26] as well as Tvrdá et al. [21] that ALB could be an important seminal antioxidant protein acting through its multiple-binding sites and free radical-trapping properties. Numerous literature sources indicate that LPO could be a primary cause of a decreased seminal quality in fertility disorders [13, 20, 24, 27].
    [Show abstract] [Hide abstract] ABSTRACT: Mutual relationships between selected chemical elements (Na, K, Fe, Cu, Mg, and Zn), basic motility characteristics (motility and progressive motility), and markers of the oxidative balance (superoxide dismutase, catalase, glutathione, albumin, and malondialdehyde) were investigated in bovine seminal plasma and spermatozoa. Computer assisted sperm analysis was used to assess the motility parameters; mineral concentrations were determined by the voltammetric method and flame absorption spectrophotometry; antioxidants and malondialdehyde were evaluated by UV/VIS spectrophotometry. Concentrations of chemical elements in both seminal fractions were in the following descending order: Na > K > Zn > Mg > Fe > Cu. Higher amounts of all minerals and nonenzymatic antioxidants were detected in the seminal plasma (; ), while higher MDA concentration and activity of enzymatic antioxidants were recorded in the cell lysates (; ). Na, Fe, Cu, Mg, and Zn were positively correlated with the motility and antioxidant parameters (; ; ). Inversely, K exhibited the positive associations with malondialdehyde (). This study demonstrates that most chemical elements are integral components of bovine semen and are needed for the protection against oxidative stress development.
    Full-text · Article · Sep 2013
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