Article

DNA damage in peripheral blood mononuclear cells of patients undergoing anti-tuberculosis treatment

Department of Tropical Diseases, São Paulo State University, Brazil.
Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis (Impact Factor: 3.68). 04/2012; 747(1):82-5. DOI: 10.1016/j.mrgentox.2012.04.003
Source: PubMed

ABSTRACT

Tuberculosis (TB), a chronic infectious disease, is a major cause of morbidity and mortality worldwide. Expression of iNOS and consequent production of NO during the inflammatory process is an important defense mechanism against TB bacteria. We have tested whether pulmonary TB patients undergoing anti-tuberculosis treatment present DNA damage, and whether this damage is related to oxidative stress, by evaluating total hydrophilic antioxidant capacity and iNOS expression. DNA damage in peripheral blood mononuclear cells from patients and healthy tuberculin test (PPD) positive controls was evaluated by single-cell gel electrophoresis (comet assay), and iNOS expression was measured by qPCR. We also evaluated total hydrophilic antioxidant capacity in plasma from patients and controls. Compared to controls, pulmonary TB patients under treatment presented increased DNA damage, which diminished during treatment. Also, the antioxidant capacity of these individuals was increased at the start of treatment, and reduced during treatment. TB patients showed lower iNOS expression, but expression tended to increase during treatment. Our results indicate that pulmonary TB patients under anti-TB treatment exhibit elevated DNA damage in peripheral blood mononuclear cells. This damage was not related to nitric oxide but may be due to other free radicals.

Download full-text

Full-text

Available from: Damiana Tortolero Pierine, Feb 10, 2014
  • [Show abstract] [Hide abstract]
    ABSTRACT: The Side Effects of Drugs Annuals form a series of volumes in which the adverse effects of drugs and adverse reactions to them are surveyed. The series supplements the contents of Meyler's Side Effects of Drugs: the International Encyclopedia of Adverse Drug Reactions and Interactions. This review of the 2011 publications on drugs used in tuberculosis and leprosy covers dapsone, ethambutol, ethionamide, isoniazid, PA-824, pyrazinamide, and the rifamycins rifabutin, rifampicin, and rifaximin, and includes a special review on concomitant treatment of multidrug-resistant tuberculosis and human immunodeficiency virus (HIV) infection.
    No preview · Article · Dec 2014 · Side Effects of Drugs Annual
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Jorge Lobo's disease, also known as lacaziosis, is a cutaneous-subcutaneous mycosis with chronic evolution. It is caused by the fungus Lacazia loboi. Herein we report a study that relates the genotoxicity caused by L. loboi in isogenic mice with nutritional status, through a normal or restricted diet. DNA damage was assessed in the peripheral blood by the comet assay (tail intensity). The results for leukocytes showed increases in the mean tail intensity in mice under dietary restriction, in infected mice under dietary restriction and in infected mice ingesting a normal diet. These results indicate that dietary restriction and L. loboi infection may increase DNA damage levels in mice, as detected by the comet assay.
    Full-text · Article · Mar 2015 · Journal of Venomous Animals and Toxins including Tropical Diseases
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: ABSTRACT Macrophages are key type of antigen-presenting cells that arbitrate the first line of defense against various intracellular pathogens. Tuberculosis, both pulmonary and extrapulmonary, is an infectious disease of global concern caused by Mycobacterium tuberculosis. The bacillus is a highly successful pathogen and has acquired various strategies to downregulate critical innate-effector immune responses of macrophages, such as phagosome-lysosome fusion, autophagy, induction of cytokines, generation of reactive oxygen and nitrogen species and antigen presentation. In addition, the bacilli also subvert acquired immunity. In this review, we aim to provide an overview of different antimycobacterial immune functions of macrophage and the strategies adopted by the bacilli to manipulate these functions to favor its survival and replication inside the host.
    Full-text · Article · May 2015 · Future Microbiology
Show more