Video S1 Synchronized video recording and activity
output of a MeCP2
mouse. This video shows a 1 minute
segment of a MeCP2
mouse in its home cage environment.
Shown below the video is the activity output plot generated by the
DSI analysis program that is synchronized to the video recording.
Note the concordance of the ambulation activity of the mouse with
the peaks depicted in the plot.
Figure S1 Automated detection of epileptiform dis-
charges. Panel A: Raw 10-second EEG waveform segment
collected from a representative MeCP2
mouse displaying 2
epileptiform discharges as determined and confirmed by visual
inspection (Red lines represent the start and end of the respective
discharge event). Panel B: Resulting envelope of the EEG
waveform in Panel A after band pass filtering the signal through
a 6–10 Hz FIR filter and then convoluting the square of this
filtered data with a Gaussian kernel of 200 point aperture (Red
lines represent the start and end of the respective discharge events,
the green line represents the envelope of the black 6–10 Hz FIR
band pass filtered signal). Panel C: Resulting derivative of the
convolved envelope signal presented in Panel B used to determine
the start and end of the discharge event. The red lines denote the
left and right inflection points used to determine the start and end
of the discharges, respectively.
Figure S2 Recognition of periodic variations in EEG,
gross motor activity, and core body temperature. Panels
A and B: Representative traces of cortical delta power patterning
over the light (i) and dark (ii) phases of a 24 hour day in a wild-type
(A) and a MeCP2
(B) mouse. Shaded regions denote areas
classified as high delta states and non-shaded regions denote areas
classified as low (non) delta states. Panels C and D: Representative
traces of mobility patterning over a 24 hour day in a wild-type (C)
and a MeCP2
(D) mouse. Shaded regions denote areas
classified as mobile behavioral states whereas non-shaded regions
denote areas classified as inactive behavioral states. Panels E and
F: Representative traces of core body temperature patterning over
a 24 hour day in a wild-type (E) and a MeCP2
Shaded regions denote areas where body temperature was above
the daily mean value, whereas non-shaded regions denote areas
where body temperature was below the mean.
Figure S3 Illustration of smoothed data generated from
raw delta power, mobility, and body temperature
traces. Panels A–D: Representative traces of raw cortical delta
power (grey line) and the resulting smoothed data (black line) as
generated using the 50-point Fast Fourier Transformation (FFT)
smoothing function in OriginPro 6.1 (OriginLab Corporation,
Northampton, MA) for a wild-type (A and B) and a MeCP2
and D) mouse during the day (A and C) and night (B and D)
phases of the 24-hour day. Panels E–H: Representative traces of
raw mobility (grey line) and the resulting smoothed data (black
line) as generated above for a wild-type (E and F) and a MeCP2
(G and H) mouse during the day (E and G) and night (F and H)
phases of a 24-hour day. Panels I–L: Representative traces of raw
core body temperature (grey line) and the resulting smoothed data
(black line) as generated above for a wild-type (I and J) and a
(K and L) mouse during the day (I and K) and night (J
and L) phases of a 24-hour day.
We thank members of the Eubanks, Zhang, and Bardakjian labs for helpful
discussions and manuscript review, and Dr. John Christodoulou for helpful
comments. We also thank Ms. Alyssa Gagnon for technical assistance.
Conceived and designed the experiments: JHE LZ BLB. Performed the
experiments: RGW SC CW LZ. Analyzed the data: JHE RGW SC BLB
LZ. Contributed reagents/materials/analysis tools: JHE CW BLB LZ.
Wrote the paper: JHE RGW SC LZ BLB.
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PLoS ONE | www.plosone.org 12 April 2012 | Volume 7 | Issue 4 | e35396