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Effect Of Ethanolic Extract Of Bauhinia Monandra Leaf On The Liver Of Alloxan Induced Diabetic Rats

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... After 72 h, blood samples were collected from the tail vein of the animals for evaluation of glucose levels by using a glucometer. Animals with glucose levels above 196 mg/dL (11.1mmol/L) were used for this study, i.e., those presenting glucose levels below 196 mg/dL, were excluded from this study [10] . ...
... The result of this work suggests that Bauhinia monandra leaf extract in alloxan induced diabetes may have little nephrotoxic components when given up to 2g/kg i.p., This was deduced from the fact the creatinine level was elevated significantly at a higher dose. B. monandra, which has the ability to reduce blood sugar level in diabetic rats [10], may not be absolutely friendly with the kidney at high doses. Therefore, B. monandra should be taken with substances that can balance any elevated parameter and such a substance should not interfere with the blood sugar lowering ability of B. monandra. ...
... After 72 h, blood samples were collected from tail veins for evaluation of glucose levels using a glucometer. Animals with glucose levels above 196 mg/dL (11.1 mmol/L) were used for this study, i.e., those presenting glucose levels below 196 mg/dL were excluded from this study [22]. At 6 weeks posttreatment, blood samples were collected by sacrificing the animals, and the blood was collected in clean EDTA tubes, then plasma was separated by centrifugation and stored at -20°C for biochemical analysis. ...
... 1,18 The primary action of sulfonylureas is to stimulate release of insulin by the pancreatic beta cells; they also have extra pancreatic action, they may also reduce hepatic clearance of insulin, and serum glucagon levels. 19,20 The hyperglycemic effect of tamsulosin should be further evaluated in other animals models of diabetes like streptozotocin induced diabetes rats and rabbits and models of type 2 diabetes such as monogenic and polygenic rats. Future studies should also evaluate interaction of tamsulosin with insulin and other oral antidiabetic drugs. ...
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Background: To evaluate the effect of tamsulosin on blood glucose levels in euglycaemic rats and to investigate the effect of glibenclamide, tamsulosin and their combination on alloxan induced diabetic rats.Methods: Albino male wistar rats were randomly assigned into 6 groups (2 euglycaemic and 4 alloxan induced diabetic rats groups). In Euglycaemic rats either normal saline (0.5ml P.O) or tamsulosin (0.072mg/kg P.O) were given and blood glucose levels was estimated at 0 hr, 30min, 1hr, 2hr, 4hr on day 1 and at 0hr and 1hr on day 3 and day 7. Four groups of diabetic rats were given normal saline (0.5ml P.O), glibenclamide (5mg/kg P.O), tamsulosin (0.072mg/kg P.O), combination of glibenclamide and tamsulosin respectively and blood glucose levels were estimated on day 1, 3 and 7. Repeated measures ANOVA or paired ‘t ‘test were used for within group comparison and one way ANOVA or unpaired ‘t’ test were used for between group comparison.Results: In euglycaemic rats tamsulosin caused significant rise in blood glucose levels at 1 hr on all days and in diabetic rats tamsulosin itself did not cause any significant alteration in blood glucose levels. However, its combination with glibenclamide delayed the onset of hypoglycemic effect of glibenclamide & also reduced its hypoglycemic effect.Conclusions: Tamsulosin significantly increase blood glucose level in euglycaemic rats and it interact with Glibenclamide to reduce its hypoglycemic activity in diabetic rats.
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The maintenance of a healthy liver is vital to overall health of the human beings. Since the liver is involved in almost all biochemical processes and there are many different diseases that will affect it. The liver is often abused by environmental toxins, which are eating habits, alcohol and overdose of certain drugs which can damage and weaken the liver and eventually lead to many diseases. Medicinal herbs are significant source of hepatoprotective drugs. Mono and poly-herbal preparations have been used in various liver disorders. According to one estimate, more than 700 mono and poly-herbal preparations in the form of decoction, tincture, tablets and capsules from more than 100 plants are in clinical use. From the literature review near about 178 medicinal plants are reported to possess a hepatoprotective activity. A drug having beneficial effect on the liver is known as hepatoprotective drug. On the other hand, drugs having toxic effect on the liver are better known as hepatotoxic drugs. The most commonly used parameters to assess the hepatoprotective activity are morphological e.g. Liver weight and volume, biochemical estimations, such as measurement of transaminase activity, SGPT, SCOT, alkaline phosphatase, serum bilirubin, total serum proteins, albumin, globulin and prothrombin time, functional parameters, pentobarbitone and hexobarbitone sleeping time and finally histopathological study regarding presence of necrosis, fatty degeneration and cirrhosis. In this review, we will briefly discuss hepatotoxicity and hepatoprotective agents.
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v>Oral administration of 1g/kg stem bark extract of Bauhinia monandra to groups of glucose - loaded and alloxan diabetic rats gave a significant anti-diabetic activity (P<0.05) compared to control rats. Peak effect (35.6% inhibition) was observed at 180mins. A peak response of 36.1% reduction in blood glucose was observed on 6th day of treatment which was significant at P<0.05.
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Natural remedies from medicinal plants are considered to be effective and safe alternative treatment for liver toxicity. Ouraim was to demonstrate the hepatoprotective effect of alcoholic and water extract of Annona squamosa (custard apple) hepatotoxicanimals with a view to explore its use for the treatment of hepatotoxicity in human. These extracts were used to study theHepatoprotective effect in isoniazid + rifampicin induced hepatotoxic model. There was a significant decrease in total bilirubinaccompanied by significant increase in the level of total protein and also significant decrease in ALP, AST, ALT and γ-GT intreatment group as compared to the hepatotoxic group. In the histopathological study the hepatotoxic group showed hepatocyticnecrosis and inflammation in the centrilobular region with portal triaditis. The treatment group showed minimal inflammation withmoderate portal triaditis and their lobular architecture was normal. It should be concluded that the extracts of Annona squamosa werenot able to revert completely hepatic injury induced by isoniazid + rifampicin, but it could limit the effect of these drugs in liver. Theeffect of extracts compared with standard drug silymarin.
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Alloxan and streptozotocin are widely used to induce experimental diabetes in animals. The mechanism of their action in B cells of the pancreas has been intensively investigated and now is quite well understood. The cytotoxic action of both these diabetogenic agents is mediated by reactive oxygen species, however, the source of their generation is different in the case of alloxan and streptozotocin. Alloxan and the product of its reduction, dialuric acid, establish a redox cycle with the formation of superoxide radicals. These radicals undergo dismutation to hydrogen peroxide. Thereafter highly reactive hydroxyl radicals are formed by the Fenton reaction. The action of reactive oxygen species with a simultaneous massive increase in cytosolic calcium concentration causes rapid destruction of B cells. Streptozotocin enters the B cell via a glucose transporter (GLUT2) and causes alkylation of DNA. DNA damage induces activation of poly ADP-ribosylation, a process that is more important for the diabetogenicity of streptozotocin than DNA damage itself. Poly ADP-ribosylation leads to depletion of cellular NAD+ and ATP. Enhanced ATP dephosphorylation after streptozotocin treatment supplies a substrate for xanthine oxidase resulting in the formation of superoxide radicals. Consequently, hydrogen peroxide and hydroxyl radicals are also generated. Furthermore, streptozotocin liberates toxic amounts of nitric oxide that inhibits aconitase activity and participates in DNA damage. As a result of the streptozotocin action, B cells undergo the destruction by necrosis.
Histochemistry and tissue pathology; principles and techniques. Claverianum press
  • Og Avwioro
Avwioro OG (2010). Histochemistry and tissue pathology; principles and techniques. Claverianum press. Ibadan, Nigeria 2 nd eition
Determining the genotoxicity of an aqueous infusion of Bauhinia monandra leaves
  • Mfs Macedo
Macedo MFS (2008). Determining the genotoxicity of an aqueous infusion of Bauhinia monandra leaves. Rev. bras. farmacogn. 18; 4:509-516.
Herbal Products for Liver Diseases: A Therapeutic Challenge for the New Millennium Essen AI β-Carotene and some characteristics of under-exploited seed oils of forest trees in Nigeria
  • Sj Detlef
  • Jia
  • B Benno
  • Gh Eckhart
Detlef SJ Jia, Benno B, Eckhart GH (1999). Herbal Products for Liver Diseases: A Therapeutic Challenge for the New Millennium Essen AI, Fetuga BL (1989). β-Carotene and some characteristics of under-exploited seed oils of forest trees in Nigeria, Food. Chemistry, England, 32:109-116.