Diversity of Cellulolytic Microbes and the Biodegradation of Municipal Solid Waste by a Potential Strain

Central Pollution Control Board, New Delhi, India.
International Journal of Microbiology 02/2012; 2012(1):325907. DOI: 10.1155/2012/325907
Source: PubMed


Municipal solid waste contains high amounts of cellulose, which is an ideal organic waste for the growth of most of microorganism as well as composting by potential microbes. In the present study, Congo red test was performed for screening of microorganism, and, after selecting a potential strains, it was further used for biodegradation of organic municipal solid waste. Forty nine out of the 250 different microbes tested (165 belong to fungi and 85 to bacteria) produced cellulase enzyme and among these
Trichoderma viride
was found to be a potential strain in the secondary screening. During the biodegradation of organic waste, after 60 days, the average weight losses were 20.10% in the plates and 33.35% in the piles. There was an increase in pH until 20 days. pH however, stabilized after 30 days in the piles. Temperature also stabilized as the composting process progressed in the piles. The high temperature continued until 30 days of decomposition, after which the temperature dropped to



C and below during the maturation. Good quality compost was obtained in 60 days.

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    • "Cellulase screening was performed by inoculating the isolates on to the mineral salt medium containing 1% cellulose and 2% agar. Plates were incubated at 50˚C for 24 hours and thereafter the Petriplates were stained with 0.1% Congo red for 1 hour followed by destaining with 1M NaCl (Gautam et al., 2012). Clearance zone was recorded (mm) around the colony. "
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    ABSTRACT: Extreme environments merit special attention and significance because of the possible existence of thermophilic microorganisms in such ecological niches. Keeping this in mind indigenous stove ash samples were explored for extremophilic bacteria in term of their biodiversity. Accordingly, this study reports 37 bacterial isolates from the local wood run oven (Tandoor) ash samples. All the isolated strains belong to genus Bacillus on the bases of morpho-cultural and biochemical considerations. The average temperature tolerance profile was >45°C thereby, indicating towards the thermophilic nature of the isolated strains. The Bacillus isolates were screened for 10 different hydrolytic enzymes (cellulase, xylanase, amylase, pectinase, caseinase, keratinase, lipase, esterase, dextranase and β-galactosidase) by plate screening method using the medium incorporated with specific substrate(s). It was found that keratinase was produced by all the isolates while, 36 (97.2%) isolates showed caseinase and esterase production. Amylase was produced by 35(94.6%) isolates and 34 (91.8%) isolates were able to degrade Tween-80 and xylan as substrate for lipase and xylanase respectively. The enzyme, β-galactosidase was produced by 31 (89.1%) of the isolates. Cellulase and dextranase were produced by 26 (70.2%) and 22 (59.4%) isolates respectively. None of the isolates could (under the existing conditions) produce pectin-hydrolyzing enzyme. According to the tukey's post hoc test, significant difference was found between the mean enzyme index of all the (screened) enzymes. Thus, the isolated bacterial strains with diverse hydrolytic potential may be of great value and relevance for the existing (national) industrial setups.
    Full-text · Article · Dec 2015 · Pakistan journal of pharmaceutical sciences
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    • "The hydrolytic potential of an indigenous strain MS28 of Alternaria has been reported (Sohail et al., 2009) and the production and partial characterization of cellulases are described (Sohail et al., 2011). Cellulase enzyme production and optimization study were reported from different Alternaria sps including Alternaria helianthi, Alternaria triticina, Alternaria sesame (Bhaskaran and Kandaswamy, 1978; Dawar and Jain, 2010; Jahangeer et al., 2005; Jha and Gupta, 1988; Marimuthu et al., 1974), A. alternata (Macris, 1984; Anand et al., 2008; Hubballi et al., 2011; Gautam et al., 2012; Saleem et al., 2013), Alternaria brassicae (Jain and Dhawan, 2008; Garg et al., 1999). "

    Full-text · Article · Sep 2014
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    • "Mucor sp. was not isolated from saw dust samples. This finding is in line with previously reported studies [22–26] that members of the genera Aspergillus and Trichoderma were the dominant fungi in forest and agricultural soils. Fungi have many different functions in soils, which include either active roles, such as the degradation of dead plant material, or inactive roles where propagules are present in the soil as a resting stage [27]. "
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    ABSTRACT: The aim of this work is to select filamentous fungal strains isolated from saw dust, soil, and decaying wood with the potential to produce xylanase and cellulase enzymes. A total of 110 fungi were isolated. Fifty-seven (57) of these fungi were isolated from soil samples, 32 from sawdust, and 19 from decaying wood. Trichoderma and Aspergillus had the highest relative occurrence of 42.6% and 40.8%, respectively. Trichoderma viride Fd18 showed the highest specific activity of 1.30 U mg(-1) protein for xylanase, while the highest cellulase activity of 1.23 U mg(-1) was shown by Trichoderma sp. F4. The isolated fungi demonstrated potential for synthesizing the hydrolytic enzymes.
    Full-text · Article · Dec 2013
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