Influence of the MCT1-T1470A polymorphism (rs1049434) on blood lactate accumulation during different circuit weight trainings in men and women
Sport Sciences Institute, Camilo Jose Cela University, Madrid, Spain. Electronic address: .
Journal of science and medicine in sport / Sports Medicine Australia
04/2012; 15(6):541-7. DOI: 10.1016/j.jsams.2012.03.009
To analyze the effect of the MCT1 T1470A polymorphism (rs1049434) on venous blood lactate levels in men and women, during three different circuit weight training protocols.
Cross-sectional laboratory study.
14 women and 15 men, all caucasian and moderately active, performed three circuit training sessions (Weight Machine Protocol, Free Weight Protocol and Combined Protocol) at 70% of the 15 repetition maximum and 70% of the heart rate reserve, in non-consecutive days. The sessions included three sets of a circuit of eight exercises. Venous lactate measurements were obtained after each set and during the recoveries between sets (i.e. in min 3, 5, 7 and 9). One-way analysis of covariance and one-way analysis of covariance with repeated measures were used to determine differences among genotypes (AA, TA and TT) in lactate levels.
In men, the AA group had higher lactate values than the TT group in all the measures (p≤0.03) except for the average lactate during the Weight Machine Protocol, in which a borderline significant difference was found (p=0.07). We did not observe differences across genotypes in females.
Our data suggest an influence of the MCT1 polymorphism on lactate transport across sarcolemma in males. Future studies on lactate transport and metabolism should take into account the gender-specific results.
Available from: Augusto G. Zapico
- "A group of professionals and researchers with expertise in a specific field: physical activity and fitness, nutrition, body composition, genetics and biochemical markers have designed together the study protocols after an in-depth review of the state of the art in the literature [8-40]. Exercise protocols were tested in healthy subjects and study protocols harmonized in a pilot study [70,71]. "
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At present, scientific consensus exists on the multifactorial etiopatogenia of obesity. Both professionals and researchers agree that treatment must also have a multifactorial approach, including diet, physical activity, pharmacology and/or surgical treatment. These two last ones should be reserved for those cases of morbid obesities or in case of failure of the previous ones. The aim of the PRONAF study is to determine what type of exercise combined with caloric restriction is the most appropriate to be included in overweigth and obesity intervention programs, and the aim of this paper is to describe the design and the evaluation methods used to carry out the PRONAF study.
One-hundred nineteen overweight (46 males) and 120 obese (61 males) subjects aged 18–50 years were randomly assigned to a strength training group, an endurance training group, a combined strength + endurance training group or a diet and physical activity recommendations group. The intervention period was 22 weeks (in all cases 3 times/wk of training for 22 weeks and 2 weeks for pre and post evaluation). All subjects followed a hypocaloric diet (25-30% less energy intake than the daily energy expenditure estimated by accelerometry). 29–34% of the total energy intake came from fat, 14–20% from protein, and 50–55% from carbohydrates. The mayor outcome variables assesed were, biochemical and inflamatory markers, body composition, energy balance, physical fitness, nutritional habits, genetic profile and quality of life. 180 (75.3%) subjects finished the study, with a dropout rate of 24.7%. Dropout reasons included: personal reasons 17 (28.8%), low adherence to exercise 3 (5.1%), low adherence to diet 6 (10.2%), job change 6 (10.2%), and lost interest 27 (45.8%).
Feasibility of the study has been proven, with a low dropout rate which corresponds to the estimated sample size. Transfer of knowledge is foreseen as a spin-off, in order that overweight and obese subjects can benefit from the results. The aim is to transfer it to sports centres. Effectiveness on individual health-related parameter in order to determine the most effective training programme will be analysed in forthcoming publications.
Available from: Bernd Wolfarth
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ABSTRACT: This review of the exercise genomics literature emphasizes the highest quality articles published in 2011. Given this emphasis on the best publications, only a small number of published articles are reviewed. One study found that physical activity levels were significantly lower in patients with mitochondrial DNA mutations compared with controls. A two-stage fine-mapping follow-up of a previous linkage peak found strong associations between sequence variation in the activin A receptor, type-1B (ACVRIB) gene and knee extensor strength, with rs2854464 emerging as the most promising candidate polymorphism. The association of higher muscular strength with the rs2854464 A allele was confirmed in two separate cohorts. A study using a combination of transcriptomic and genomic data identified a comprehensive map of the transcriptomic features important for aerobic exercise training-induced improvements in maximal oxygen consumption, but no genetic variants derived from candidate transcripts were associated with trainability. A large-scale de novo meta-analysis confirmed that the effect of sequence variation in the fat mass and obesity-associated (FTO) gene on the risk of obesity differs between sedentary and physically active adults. Evidence for gene-physical activity interactions on type 2 diabetes risk was found in two separate studies. A large study of women found that physical activity modified the effect of polymorphisms in the lipoprotein lipase (LPL), hepatic lipase (LIPC), and cholesteryl ester transfer protein (CETP) genes, identified in previous genome-wide association study reports, on HDL cholesterol. We conclude that a strong exercise genomics corpus of evidence would not only translate into powerful genomic predictors but also have a major effect on exercise biology and exercise behavior research.
Available from: Ildus I. Ahmetov
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ABSTRACT: Purpose: In red skeletal muscle monocarboxylate transporter 1 (MCT1) is required for lactate to enter the myocytes for oxidation. The A1470T polymorphism (rs1049434) in MCT1 gene was shown to be associated with lactate transport rates in human skeletal muscles. The aim of the study was to compare genotype and allele frequencies of the MCT1 gene polymorphism in 323 Russian athletes and 467 non-athletic controls, and to investigate the association of the MCT1 gene A1470T polymorphism with maximal oxygen consumption and maximal lactate concentration in rowers (n = 79).
Methods: Genotyping for the A1470T MCT1 polymorphism was performed by PCR-RFLP method. Physiological measurements of 79 Russian rowers of national competitive standard were determined during an incremental test to exhaustion on a rowing ergometer.
Results: Frequencies of the A allele (71.8 vs. 62.5%, P < 0.0001) and AA genotype (59.8 vs. 39.4%, P < 0.0001) were significantly higher in endurance-oriented athletes (n = 142) compared with the control group. Mean blood lactate concentration was higher in male rowers with the T allele (AT+TT – 10.26 ± 1.89 mmol*l-1, AA – 8.75 ± 1.69 mmol*l-1, P = 0.005).
Conclusions: We have shown that the MCT1 gene A1470T polymorphism is associated with endurance athlete status and blood lactate level after an intensive exercise in athletes.
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