Atrazine and PCB 153 and their effects on the proteome of subcellular fractions of human MCF-7 cells

Centre de Recherche Public - Gabriel Lippmann, Department of Environment and Agro-Biotechnologies, 41, rue du Brill, L-4422 Belvaux, Luxembourg.
Biochimica et Biophysica Acta (Impact Factor: 4.66). 04/2012; 1824(6):833-41. DOI: 10.1016/j.bbapap.2012.03.014
Source: PubMed


Several man-made organic pollutants including polychlorinated biphenyls (PCBs) and several pesticides may exhibit endocrine disrupting (ED) properties. These ED molecules can be comparatively persistent in the environment, and have shown to perturb hormonal activity and several physiological functions. The objective of this investigation was to study the impact of PCB 153 and atrazine on human MCF-7 cells, and to search for marker proteins of their exposure. Cells were exposed to environmentally high but relevant concentrations of atrazine (200ppb), PCB 153 (500ppb), 17-β estradiol (positive control, 10nM) and DMSO (0.1%, negative control) for t=36h (n=3 replicates/exposure group). Proteins from cell membrane and cytosol were isolated, and studied by 2D-DiGE. Differentially regulated proteins were trypsin-digested and identified by MALDI-ToF-ToF and NCBInr database. A total of 36 differentially regulated proteins (>|1.5| fold change, P<0.05) were identified in the membrane fraction and 22 in the cytosol, and were mainly involved in cell structure and in stress response, but also in xenobiotic metabolism. 67% (membrane) and 50% (cytosol) of differentially regulated proteins were more abundant following atrazine exposure whereas nearly 100% (membrane) and 45% (cytosol) were less abundant following PCB 153 exposure. Western blots of selected proteins (HSBP1, FKBP4, STMN1) confirmed 2D-DiGE results. This study emphasizes the numerous potential effects that ED compounds could have on exposed humans.

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    • "Atrazine (6-chloro-N 2 -ethyl-N 4 -isopropyl-1,3,5-triazine-2,4-diamine, ATR) is in many countries, including the United States and Australia, a commonly used s-triazine herbicide for controlling broadleaf and grassy weeds in agricultural soils primarily for the cultivation of corn (Williams et al., 2011) and sugarcane (Masters et al., 2013). Due to the frequent detection of ATR and its N-dealkylated degradation products in freshwater supplies worldwide (Bohn et al., 2011; Drevenkar et al., 2004; Garmouma et al., 1997; Jin and Peldszus, 2012; Kumar and Xagoraraki, 2010; Segura et al., 2011), and the potentially adverse impacts on animal (Zaya et al., 2011) and human health (Lasserre et al., 2012), ATR has been banned in the countries of the European Union (EU). In the United States, ATR is classified as a restricted-use herbicide in order to minimize its potential to reach ground and surface waters. "
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    ABSTRACT: Field experiments were carried out to compare the effect of herbicide application rate on the distribution and persistence of atrazine (ATR), terbuthylazine (TBA), and their dealkylated degradation products in two conventionally cultivated cornfield soils: a silt loam soil and a silty clay loam soil, both typical for the crop-producing area of continental Croatia. Herbicides were applied pre-emergence at the registered (R) (1.0-1.5 kg ha-1), double-registered (2R), and triple-registered (3R) rates. Topsoil (0-30cm) and subsoil (30-60 cm) samples were collected periodically during a crop rotation cycle of 17months. Soil microcosm experiments were performed under controlled conditions to investigate the influence of the herbicide application rate on their biodegradation in herbicide-treated cornfield topsoils. The herbicide half-lives (DT50) calculated by using monophasic and biphasic first-order kinetics models were compared. The herbicide sorption intensity in the studied soils was evaluated in terms of the Freundlich sorption isotherm. Under field conditions, TBA was retained within the first 60cm of both agricultural soils at least twice as long as ATR. While ATR residues persisted in soil only until corn harvest (5months), the residues of TBA and its N-deethylated degradation product were still measurable at the end of the crop rotation cycle (17 months). The persistence of both herbicides was higher in soil with higher herbicide sorption intensity. ATR and TBA application rates higher than registered generally did not significantly influence the herbicide biodegradation rates or dissipation from the topsoil layers. The enhanced application rate slightly decreased the TBA persistence only in soil exhibiting a rather slow biodegradation. A temperature-leaching potential index for ATR and TBA applied at R rate showed the same leachability class for both herbicides. The leachability class of TBA could be higher than that of ATR in soil with moderate TBA persistence (DT50=30-100 days). From an environmental perspective, TBA could pose a similar risk for groundwaters as ATR.
    No preview · Article · Dec 2015 · Geoderma
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    • "Atrazine (2-chloro-4-[ethylamino]-6-[isopropylamino]-s-triaz- ine) herbicide belongs to the triazine family, and has been widely used to control weeds in crops. Atrazine is highly mobile toxic to aquatic organisms, plants, and human beings (Lasserre et al., 2012). Techniques for removing triazine from aqueous solutions 0960-8524/Ó 2015 Elsevier Ltd. "
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    ABSTRACT: The physicochemical properties of biochars produced from soybeans (SBB), corn stalks (CSB), rice stalks (RSB), poultry manure (PMB), cattle manure (CMB), and pig manure (PgMB) and their adsorption characteristics of atrazine were investigated. The adsorption capacity increased with the increase of temperature and initial atrazine concentration. More atrazine was removed from basic solutions than acidic solutions, due to the effects of adsorption and hydrolysis. The Freundlich isotherm adsorption parameters indicated that the adsorption capacity decreased in the order SBB>RSB>CMB>CSB>PMB>PgMB, which is associated to the pore volume of biochars. The total pore volume and biochar pH were concluded to play important roles in determining the adsorption capacity, and they may have contributed to physical adsorption mechanisms dominating the overall adsorption process (the low activation energy for all of the biochars). Modified Freundlich and intraparticle diffusion models were used to describe the kinetics of the adsorption process.
    Full-text · Article · Sep 2015 · Bioresource Technology
    • "It is the most commonly detected pesticide in ground and drinking water with low bioaccumulation in aquatic organisms, but relatively high persistence in the environment (half-life 237 days) [4]. Atrazine is considered by the United States Environmental Protection Agency (USEPA) as potential carcinogen [5] and acts as environmental endocrine disruptor [6] [7]. Exposure to atrazine from drinking water causes menstrual cycle length irregularity and reduces reproductive hormone levels in women [8]. "
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    ABSTRACT: The occurrence of common herbicides (Atrazine, ATZ and Iodosulfuron, IDS), in waters presents potential risk to human and ecological health. The oxidative degradation of ATZ and IDS by ferrate(VI) (FeVIO42-, Fe(VI)) is studied at different pH levels where kinetically observed second-order rate constants (k app, M-1 s-1) decreased with increase in pH from 6.0 to 9.0. The determination of the rate constants for the Fe(VI) species, HFeO4- and FeO42-, with the herbicides using acid-base equilibria of Fe(VI) suggested that the HFeO4- species was largely responsible for the pH dependence behavior of k app. The title herbicides degraded >90% within 10min using excess weighted amounts of Fe(VI) over IDS (2:1) ATZ (20:1) and in the pH range of 6.0-9.0. Comparatively, degradations of ATZ and IDS by Mn(VII) were ~70% and ~40% under same conditions; - showing a superior efficiency of ferrate(VI) in degradation of toxic herbicides. The oxidized products of ATZ and IDS by Fe(VI) at a neutral pH were examined by liquid chromatography-tandem mass spectrometry (LC/MS/MS) analysis, which indicated that Fe(VI) attacked on the sulfonamide and alkyl chain moieties of ATZ and IDS, respectively.
    No preview · Article · Aug 2015 · Separation and Purification Technology
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