Chemical Reporters for Monitoring RNA Synthesis and Poly(A) Tail Dynamics

Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, 1230 York Avenue Box 250, New York, NY 10065, USA.
ChemBioChem (Impact Factor: 3.09). 05/2012; 13(8):1112-5. DOI: 10.1002/cbic.201200091
Source: PubMed


A versatile "clickable" nucleoside: Metabolic labeling of cells is useful in studying the dynamics of biological molecules. N(6) pA can be utilized by all three mammalian RNA polymerases, as well as poly(A) polymerase. Because of its alkyne modification, RNA labeled with N(6) pA can be visualized and purified by using click chemistry.

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