Promoting crystallisation of the Salmonella enteritidis fimbriae 14 pilin SefD using deuterium oxide

ArticleinBiochemical and Biophysical Research Communications 421(2):208-13 · April 2012with26 Reads
Impact Factor: 2.30 · DOI: 10.1016/j.bbrc.2012.03.136 · Source: PubMed

    Abstract

    The use of heavy water (D(2)O) as a solvent is commonplace in many spectroscopic techniques for the study of biological macromolecules. A significant deuterium isotope effect exists where hydrogen-bonding is important, such as in protein stability, dynamics and assembly. Here we illustrate the use of D(2)O in additive screening for the production of reproducible diffraction-quality crystals for the Salmonella enteritidis fimbriae 14 (SEF14) putative tip adhesin, SefD.