Article

Tumor ablation with nanosecond pulsed electric fields

Division of Hepatobiliary Pancreatic Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China. .
Hepatobiliary & pancreatic diseases international: HBPD INT (Impact Factor: 1.17). 04/2012; 11(2):122-4. DOI: 10.1016/S1499-3872(12)60135-0
Source: PubMed
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    ABSTRACT: In the last decade, high-intensity pulsed electric fields with nanosecond durations (3-300 ns) have found breakthrough biomedical applications, e.g., in cancer treatment and gene therapy; however, the physical mechanisms underlying the interaction between nanosecond pulsed electric fields (nsPEFs) and cells, tissues, or organs are not yet fully elucidated. The precise knowledge of the electromagnetic dose received by the exposed sample at the macroscopic, and better still at the microscopic scale, is essential to complete our understanding of the phenomena involved and for adequate interpretation and reproducibility of the results. In this paper, we report a dosimetric and microdosimetric study of an in vitro exposure setup based on a transverse electromagnetic (TEM) cell that allows the exposure of cells in a Petri dish to nsPEFs. The rectangular and bipolar pulses delivered to the cells had a total duration of 1.2 ns and an amplitude of 2 kV. The electric field in situ was characterized experimentally with a nonmetallic probe and numerically using a finite-difference time-domain algorithm. Results of real-time monitoring of temperature were obtained at the subcellular level by using microfluorimetry, which is a method of imaging temperature by using a fluorescent molecular probe with thermosensitive properties.
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    ABSTRACT: The cellular response to 100 ns pulsed electric fields (nsPEF) exposure includes the formation of transient nanopores in the plasma membrane and organelle membranes, an immediate increase in intracellular Ca(2+), an increase in reactive oxygen species (ROS), DNA fragmentation and caspase activation. 100 ns, 30 kV/cm nsPEF stimulates an increase in ROS proportional to the pulse number. This increase is inhibited by the anti-oxidant, Trolox, as well as the presence of Ca(2+) chelators in the intracellular and extracellular media. This suggests that the nsPEF-triggered Ca(2+) increase is required for ROS generation.
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    ABSTRACT: The literature survey 2012 is based on 1426 papers found in the databases MEDLINE and EMBASE with the keywords “thermography” or “thermometry” “temperature measurement” or “thermotherapy” or ‘skin temperature’ or ‘core temperature’ and restricted to “human” and “included in the databases between 01.01 and 31.12. 2012”. 37.9 percent of papers of this review are originated from Europe and 95.3 percent of all papers are written in English. 238 controlled studies using some kind of temperature measurement were included in this survey. Pharmacology, Internal Medicine, Cancer andNeurology&Psychiatry were the predominant fields of applications of temperature measurement in medicine. As in previous years, therapeutic hypothermia and hyperthermia treatment was the topic of many papers. Fever attracted also a high number of publications. Although the term “breast” appeared in 77 publications, only minority of those were related to breast thermography. Some articles were found for the complex regional pain syndrome and Raynaud´s phenomenon and new applications of medical thermography have been reported.
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