Selective Chemical Imaging of Static Actin in Live Cells

ArticleinJournal of the American Chemical Society 134(20):8480-6 · April 2012with190 Reads
DOI: 10.1021/ja211708z · Source: PubMed
Abstract
We have characterized rationally designed and optimized analogues of the actin-stabilizing natural products jasplakinolide and chondramide C. Efficient actin staining was achieved in fixed permeabilized and non-permeabilized cells using different combinations of dye and linker length, thus highlighting the degree of molecular flexibility of the natural product scaffold. Investigations into synthetically accessible, non-toxic analogues have led to the characterization of a powerful cell-permeable probe to selectively image static, long-lived actin filaments against dynamic F-actin and monomeric G-actin populations in live cells, with negligible disruption of rapid actin dynamics.

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