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[Activities of protein synthesis during the development of lethal nucleocytoplasmic hybrids obtained by nuclear graft between Ambystoma mexicanum Shaw and Ambystoma dumerilii Duges (Amphibia, Urodeles)]
Abstract
A comparative study of proteinograms and zymograms (LDH, MDH) has been carried out at different ontogenic stages, in Ambystoma mexicanum (A.m.), Ambystoma dumerilii (A.d.) and nucleocytoplasmic hybrids 2 n A.d/cytoplasm Am. It appears that 2 n A.d. nucleus, grafted in A.m. cytoplasm expresses only a part of its potentialities, which could account for lethality of the nucleocytoplasmic hybrids.
The ontogeny of lactate dehydrogenase (LDH) isozymes in developing Mexican axolotl, A mbystoma mexicanum was investigated by thin-layer isoelectric focusing in polyacrylamide gel. The isoelectric points (pI values) of the isozymes were determined. The minor components generally remained masked during conventional electrophoresis, but became sharp as isofocusing progressed.
We identified in developing eggs and embryos five major LDH isozymes, which could also be traced in the ovarian eggs. All these isozymes, except LDH-1, consisted of one major and one minor component. Heterogeneity in axolotl LDH is reported for the first time. The separated isozymes had pI values from 5.24–6.60. Contrary to observations made by others, it was found that the anodal forms of LDH (pIs 5.24–5.80) were prominent throughout, while the remainder (pIs 6.16–6.60) gradually lost their stain ability.
It thus appears that isoelectric focusing is a possible method for the analysis of protein mixtures and can be successfully applied to problems of differentiation.
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