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Antifungal activity of lemon (Citrus lemon L.), mandarin (Citrus reticulata L.), grapefruit (Citrus paradisi L.) and orange (Citrus sinensis L.) essential oils
Abstract
The objective of this work was to study the effect of the essential oils of lemon (Citrus lemon L.), mandarin (Citrus reticulata L.), grapefruit (Citrus paradisi L.) and orange (Citrus sinensis L.) on the growth of moulds commonly associated with food spoilage: Aspergillus niger, Aspergillus flavus, Penicillium chrysogenum and Penicillium verrucosum, using the agar dilution method. All the oils showed antifungal activity against all the moulds. Orange essential oil was the most effective against A. niger, mandarin essential oil was most effective at reducing the growth of Aspergillus flavus while grapefruit was the best inhibitor of the moulds P. chrysogenum and P. verrucosum. Citrus essential oils could be considered suitable alternatives to chemical additives for use in the food industry.
... [54] Other studies have corroborated the anti-bacterial activity of lemon essential oil against P. aeruginosa, Lactobacillus curvatus, E. ammnigenus, Mycobacterium smegmatis, Micrococcus luteus, Proteus vulgaris, Enterobacter gergoviae, S. aureus, S. xylosus, S. carnosus, Listeria monocytogenes and L. sakei. [55,56] Like lemon, bergamot essential oil has shown effective antibacterial activity against Campylobacter jejuni, and E. coli O157 and linalool a major component of the bergamot essential oil confirmed its anti-bacterial activity by inhibiting the growth of S. aureus and L. monocytogenes. [57] As expected, a different study has also reported that citrus orange essential oil can inhibit the growth of E. coli O457: H7 and Salmonella spp. ...
... Growth inhibition [67,68,74] C. [55,56,63] Antiviral Effect ...
... [61,63] As reported by several studies, different species of fungi including Aspergillus flavus, A. fumigatus, A. Niger, Alternaria alternata, cladosporium herbarum, curvularia lunata, Fusarium oxysporum, helminthosporum oryzae, Penicillium chrysogenum, penicillium overrucosum and trichoderma viride were susceptible to C. sinensis essential oil. [55,86,87] Citrus aurantium L. essential oil has reportedly shown significant inhibition against the growth of Aspergillus Niger, A. flavus, A. nidulans, A. fusarium graminearum, F. oxyporum, C. albicans, and Alternaria alternate.- [61,62,88,89] C. reticulata essential oil also showed antifungal properties by inhibiting the growth of Aspergillus flavus and it was found to cause 40% growth inhibition [93] ( Table 2). ...
Citrus essential oil (CEO) is obtained from the fruit of Genus Citrus, a flowering plant shrub in the family of the Rutaceae (Eremocitrus or Microcitrus) and extensively used in food, chemical industry, and traditional medicinal treatment owing to its pleasant aroma, antioxidant, and antiseptic properties. This review presents a botanical description, distribution, traditional uses, chemical composition, bioactive components, and the therapeutic uses as well as toxicological effects of the CEO. The objective was achieved via a comprehensive literature search of electronic databases such as Science Direct, PubMed, Web of Science, Wiley, ACS, Springer, Taylor and Francis, Google Scholar, SCOPUS, conference proceedings, thesis, and books until 2022 for publications. Citrus essential oils and their constituents are extracted and isolated either from the fruit peels, seeds, leaves, or flowers of the citrus plants. A comparative study of the sources of CEO confirmed its origin, ethnopharmacological and therapeutic uses. Over 2000 secondary metabolites have been isolated, with the main active constituents: being terpenes, monoterpenes, sesquiterpenes, and diterpenes. A comprehensive literature review revealed vast therapeutic benefits of CEO. Incomplete data report on in vitro and in vivo trials especially, on dosage, positive and negative control groups, intervention time, toxicity studies, phytochemical profiling, and clinical trials seem to be a knowledge gap.
... These concern use of EOs not only as alternative antibiotics in the medical field [12,[55][56][57][58][59][60], but particularly as food additives. The latter usage, that may also involve EOs extracted from other plants which are not considered in this article, aims at improving the quality of a wide array of food products with reference to both possible contamination with human pathogens and preservation of organoleptic properties after inhibition of deteriorating microbial agents [47,50,56,[61][62][63][64][65][66]. Of course, the latter is a priority aspect in the case of citrus fruits, which generally need to overcome prolonged postharvest periods before undergoing either fresh consumption or industrial transformation. ...
... Inhibitory effects have also been documented against other fungi, such as Aspergillus spp. [55,63,78,82,85,[93][94][95]97,101,103,104], which may affect citrus quality as mycotoxin producers [105,106]. The gray mold agent Botrytis cinerea, also recently affirmed as an emergent citrus postharvest pathogen [107], proved to be sensitive to lemon essential oil, which completely inhibits in vitro growth at concentrations of 17 µL mL −1 [83], or 0.016% [108]. ...
Citrus essential oils (EOs) are widely used as flavoring agents in food, pharmaceutical, cosmetical and chemical industries. For this reason, their demand is constantly increasing all over the world. Besides industrial applications, the abundance of EOs in the epicarp is particularly relevant for the quality of citrus fruit. In fact, these compounds represent a natural protection against postharvest deteriorations due to their remarkable antimicrobial, insecticidal and antioxidant activities. Several factors, including genotype, climatic conditions and cultural practices, can influence the assortment and accumulation of EOs in citrus peels. This review is focused on factors influencing variation of the EOs’ composition during ripening and on the implications on postharvest quality of the fruit.
... Consequently, the mandarin essential oil has potential antibacterial effect against S. aureus, one of the foremost foodborne pathogens around the world, that showed strong tolerance to low temperature (4 • C and− 20 • C), at which food is typically stored (Qiao, Zhu et al., 2020). Meanwhile, this oil had little growth inhibition effect on other bacteria commonly used in the food industry like Lactobacillus curvatus, L. sakei, Staphylococcus carnosus, and S. xylosus or related to food spoilage as Enterobacter gergoviae and E. amnigenus (Viuda-Martos, Ruiz-Navajas et al., 2008). Regarding its antifungal effect, it exhibited a potential inhibitory effect against several moulds commonly associated with food spoilage (Aspergillus niger, Aspergillus flavus, Penicillium chrysogenum, and Penicillium verrucosum) (Viuda-Martos, Ruiz-Navajas et al., 2008) and is regarded as a natural antimicrobial for food preservation. ...
... Meanwhile, this oil had little growth inhibition effect on other bacteria commonly used in the food industry like Lactobacillus curvatus, L. sakei, Staphylococcus carnosus, and S. xylosus or related to food spoilage as Enterobacter gergoviae and E. amnigenus (Viuda-Martos, Ruiz-Navajas et al., 2008). Regarding its antifungal effect, it exhibited a potential inhibitory effect against several moulds commonly associated with food spoilage (Aspergillus niger, Aspergillus flavus, Penicillium chrysogenum, and Penicillium verrucosum) (Viuda-Martos, Ruiz-Navajas et al., 2008) and is regarded as a natural antimicrobial for food preservation. This activity is correlated to the presence of limonene (reaching 78.89% in the oil) in C. reticulata essential oil (Espina, Somolinos et al., 2011;Simas, de Amorim et al., 2017;Song, Liu et al., 2020). ...
Fruits are regarded as the richest dietary sources of polyphenols, carotenoids, terpenoids, and limonoids, making them highly desired prospects for the functional food industry. Citrus waste that remains after fruit processing is also regarded as a valuable source of value-added phytochemicals. A comprehensive analysis of mandarin composition as a determinant of fruit functional properties, health benefits, and valorization into useful products is represented together with the effect of different processes such as light, wax coating, auxin addition, ozone treatment, and ethylene degreening. Such reviewing evidence is an essential precondition for improving the selling market of mandarin and highlighting its waste valorization potential and its incorporation into useful products produced by pharmaceutical industries. In this study, the phytochemical and biological reports presented to emphasize the needed priority for agricultural techniques to provide a certain mandarin product or trait. In addition, the paper summarizes the optimum conditions applied for the updated extraction techniques for phenolic acids, flavonoids, and oils at a commercial scale from mandarin peels and to enhance its global marketing. A brief representation is given towards potential mandarin patents and the valorization of its bio-waste fruits into commercial products of added value. The review can guide researchers to produce future potentially marketed functional food enriched with mandarin extracts and/or bioactives.
... Actually, the mechanism of terpenes action is not fully understood, but it is believed that these compounds are involved in the damage and stability of plasma and the subsequent membrane disruption by lipophilic compounds [35,39]. Limonene and linalool, which were found to be abundant in this study, were reported as compounds with significant antimicrobial property [45]. It has also been shown that limonene, the major compound of EOs of Citrus genus, has a weaker antibacterial effect than antifungal activity. ...
... It has also been shown that limonene, the major compound of EOs of Citrus genus, has a weaker antibacterial effect than antifungal activity. But the antimicrobial activity of Citrus EO is enhanced by the presence of bioactive alcohol, linalool, a monoterpene alcohol, known to be a potent antimicrobial [45]. On the other hand, EO activity of C. aurantium peel may be the result of a synergistic effect between these different compounds, especially since the fraction of oxygenated monoterpenes is relatively high (19.16%). ...
This study was designed to analyze the chemical composition of Citrus aurantium Essential Oil (CAEO) peels and to evaluate α-glucosidase inhibition potential, antioxidant and antibacterial activities. According to GC-MS analyses, 37 compounds were identified with limonene was the most abundant (62.2%). Majority of the identified compounds belong to hydrocarbon monoterpenes fraction (75.7%), followed by oxygenated monoterpenes (19.16%). CAEO α-glucosidase inhibition outlined an important activity with IC50 = 10±1 mg/mL. Moreover, antioxidant activity revealed that CAEO exhibited a potent scavenging effect through 2,2-diphenyl-1 picrylhydrazyl radical (DPPH.) (IC50=33.66 µg/mL) and an important ferric ion reducing antioxidant power (FRAP) activity (EC50=98.67 µg/mL). Antimicrobial data demonstrate that CAEO was active against a panel of pathogenic bacteria and that CAEO was able to destroy bacterial cells (bactericidal) according to the MBC/MIC ratios towards Gram+ and Gram- tested strains.
... Ozgoli et al. confirmed the higher efficacy of clove aromatherapy than peppermint aromatherapy in reducing levels of pain and anxiety during labor (41). The effects of Mandarin Orange essential oil aromatherapy on labor pain and anxiety: Mandarin orange (Citrus reticulata) is one of the citrus species of the Rutaceae family (56). In a study by Burns et al, it has been shown that using Mandarin essential oil in combination with four other essential oils (Clary sage, Roman chamomile, lavender, frankincense,) significantly decrease labor pain and anxiety (12). ...
Background: Family-centered empowerment model (FCEM) greatly values the motivational, psychological, and functional roles of children and their family members in health promotion. Its main goal is to strengthen the family system to promote children and family health. Aim: To investigate the effect of FCEM on knowledge and stress level among mothers of children with glucose-6-phosphate dehydrogenase enzyme deficiency(G6PD). Study design: A quasi-experimental design was performed. Setting: The study was conducted in hematology outpatient clinic at Mansoura University Children’s Hospital. Subjects: a purposive sample composed of 60 mothers and their children who are suffering from G6PD deficiency. Tools: Two tools were used for data collection: Glucose-6-Phosphate Dehydrogenase Enzyme Deficiency Knowledge Questionnaire and Arabic version of Perceived Stress Scale. Results: There were an improvement in the mean score of mothers' total knowledge with statistically significant difference (P<0.001) in the post intervention phase compared to pre intervention phase. Mean score of mothers' stress level was 26.90 ± 6.26 pre the FCEM implementation and decrease to17.36 ± 2.73 post FCEM with a statistically significant difference. Conclusion: Applying FCEM on mothers of children with G6PD deficiency leading to improvement of their knowledge regarding G6PD and reduce their level of stress Recommendations: researchers recommended implementing FCEM for all mothers and their children with chronic diseases as a strategy for education and management.
... Citrus limonum EO has been widely utilized in various applications, which include food, medicinal product, detergents, cosmetics products, microbial inhibition, and pest management (Bertuzzi, 2013;Viuda-Martos et al., 2008). The citrus essential oil has been found in various sections of fruits as well as leaves, indicating that limonene, a-pinene, p-cymene, ß-pinene, ß-myrcene, terpinolene, and other components are the main aromatic elements of several citrus species (Caccioni et al., 1998;Buettner et al., 2003). ...
... The bioactive compounds and extracted oils of Citrus have shown a high anti-fungal property [54]. Essential oils extracted from different varieties of Citrus such as orange, lemon, mandarin, and grapefruit have shown anti-fungal activities against Aspergillus niger, A. flavus, Penicillium chrysogenum, and P. verrucosum [80]. The study in Citrus sinensis oil was performed to check the hyphal cell wall and diameter characteristics of fungus. ...
Citrus, belonging to the Rutaceae family, is a commercial fruit worldwide, and it is mainly recognized for its nutritional, anti-oxidant, and significant medicinal properties. Citruses are a group of multifaceted fruit crops with a rich traditional knowledge, deeply rooted in ethnic culture, and the fruits have been considered to be health-protecting and health-promoting food supplements since ancient times. The presence of secondary metabolites and their bioactivities has led to the development of new alternative drugs in recent years. Diverse secondary metabolites such as flavonoids, alkaloids, carotenoids, phenolic acids, and essential oils and their high bioactive properties have imparted great value to human health based on their anti-oxidative, anti-inflammatory, anti-cancer, cardiovascular protective, and neuroprotective effects. The indigenous Citrus species of India—mainly Northeast India—have distinctive and valuable genetic traits, such as resistance to biotic and abiotic stress, distinctive aroma, flavor, etc. Hence, these species are considered to be repertoires of valuable genes for molecular breeding aimed at quality improvement. There is a need for awareness and understanding among the citrus-producing countries of the exploitation of biodiversity and the conservation of Citrus for sustainable development and bioprospecting. The current review presents a holistic view of Citrus biodiversity from a global perspective, including phytochemical constituents and health benefits. Advanced biotechnological and genomic approaches for Citrus trait improvement have also been discussed to highlight their relevance in Citrus improvement.
... Otros autores (Baratta et al., 1998;Chaisawadi et al., 2005;Chanthaphon et al., 2008;Sharma y Tripathi, 2008;Shukla et al., 2000;Quintana et al., 2010;Viuda-Martos et al., 2008) demuestran actividad inhibitoria del crecimiento y/o germinación de hongos, con análisis antifúngicos a diferentes condiciones, a partir de aceites esenciales de canela, orégano, mejorana, limoncillo, limón, mandarina, pomelo (Citrus paradisi L.) y naranja dulce. Algunos autores mencionan que un posible mecanismo de acción de los aceites sobre el crecimiento de los hongos es debido a los constituyentes con alta hidrofobicidad presentes en la célula, lo que ocasiona trastornos en la permeabilidad y en el transporte de iones y de otros compuestos, así como en la separación de componentes lipídicos de la membrana celular y la mitocondria (Quintana et al., 2010). ...
Se estudió la conservación de frutos de pepino Cucumis sativus L. con recubrimientos comestibles a base de almidón de yuca y quitosano, incorporándole cinamaldehído. Las muestras fueron tratadas con recubrimientos de almidón y quitosano al 1 % (p/v) y aceite esencial en concentraciones de 0,05 % y 0,15 % (v/v), respectivamente. Las muestras se almacenaron a 10 °C y 80 % de humedad relativa durante 21 días. Se evaluó sólidos solubles, acidez titulable, pH, firmeza y pérdida de peso cada 24 horas, y los análisis microbiológicos se evaluaron cada 72 horas. Se determinó que el tratamiento T4 (quitosano 1% + cinamaldehído 0,15%) extendió la vida útil de los frutos de pepino en 8 días en comparación con los pepinos sin recubrimiento (control). Adicionalmente, se observó que los tratamientos presentaron mayor firmeza y menor pérdida de peso en comparación con el control. En relación con el análisis microbiológico de mohos y levaduras, los resultados demuestran que los tratamientos T3 (quitosano 1 % + cinamaldehído 0,05 %), y T4 (quitosano 1 % + cinamaldehído 0,15 %) fueron estadísticamente diferentes (p < 0,05) a los otros tratamientos y al control durante los 21 días de almacenamiento. Los resultados obtenidos en este estudio expresan la eficacia de los recubrimientos comestibles de quitosano con cinamaldehído como método de conservación en postcosecha del pepino.
... A possible mechanism of action for plant essential components on the growth of fungi has been reported in several studies. It is generally accepted that the essential oil components act on the functionality and the structure of the cell membrane [82]. Low concentrations result in changes in the cell's structure, inhibiting respiration and changing the permeability of the cell membrane, whereas high concentrations lead to severe membrane damage, loss of homeostasis and cell death [83]. ...
... Citrus spp. EOs have been reported to exhibit a wide spectrum of biological activity, including anti-carcinogenic, anti-bacterial, antioxidant, anti-fungal, anti-microbial, anti-spasmodic, anti-diabetic, anti-dermatophyte, and anti-inflammatory activities [7][8][9][10]. ...
This study evaluates the applicability of enantioselective gas chromatography (eGC) and enantioselective comprehensive two-dimensional gas chromatography (eGC×GC) coupled with flame ionization detection for the stereospecific analysis of designated chiral monoterpenes within essential oils distilled from the leaves of Citrus hystrix (CH), C. limon (CL), C. pyriformis (CP), and C. microcarpa (CM). A cryogen-free solid-state modulator with a combination of enantioselective first-dimension and polar second-dimension column arrangements was used to resolve potential interferences in Citrus spp. leaf oils that can complicate the accurate determination of enantiomeric compositions. Interestingly, considerable variations were observed for the enantiomeric fractions (EFs) of the chiral terpenes. (+)-limonene was identified as the predominant enantiomer (60.3–98.9%) in all Citrus oils, (+)-linalool was the major enantiomer in CM (95.9%), (−)-terpenin-4-ol was the major isomer in CM (66.4%) and CP (61.1%), (−)-α-pinene was the dominant antipode in CL (55.5%) and CM (92.1%). CH contained (−)-citronellal (100%) as the pure enantiomer, while CL and CP have lower proportions (9.0–34.6%), and citronellal is absent in CM. The obtained enantiomeric compositions were compared and discussed with results from eGC using the same enantioselective column. To our knowledge, this work encapsulates the first report that details the EFs of these chiral monoterpenes in Citrus spp. leaf oil.
... (USFDA), se pueden usar con películas y recubrimientos comestibles hasta el límite de lo que proporcionan los máximos efectos protectores sin mucho impacto en las propiedades sensoriales y organolépticas de los alimentos (Viuda-Martos et al., 2008). Por lo tanto, los directores ejecutivos poseen un inmenso potencial para sus aplicaciones en la seguridad, el envasado y la conservación de los alimentos. ...
Essential oils are compounds that are formed by several lipidic aromatic substances found in different parts of a plant. There are many plants with different essential oils and different extraction methods are used to take advantage of the diverse properties they offer. In recent times, the resistance of microorganisms to antibiotics opens the interest to search for new routes of antimicrobial control of natural origin, and within them, essential oils have enormous potential. They have a wide variety of chemical compounds that act establishing synergism relationships to perform antimicrobial, antioxidant, antifungal, antiviral, and other activities that characterize them. Citrus essential oils are an economical, ecological, and natural alternative to chemical preservatives. The extraction of citrus oils from waste peels not only protects the environment, but can also be used in various applications, including food preservation. Current needs to reduce allergenic effects caused by certain foods demand urgent research in this area; studies and applications of citrusderived essential oils show a clear technological and ecological alternative to solve this problem.
... The Lemon grass volatile oil showed the greatest antifungal activity against Aspergillus flavus at concentrations of (0.006-0.03%) by inhibiting the fungal growth and aflatoxin production [39]. The antifungal abilities of Lemon, Mandarin, Grapefruit, and orange oils at a concentration of < 1% were reported against different species of Aspergillus and Penicillium molds [56]. Different EOs and their ingredients showed the most antifungal effects versus C. albicans drug-resistant biofilms by suppressing membrane ergosterol and varied signaling paths that prevent hyphal morphogenesis from the yeast [57]. ...
... The Lemon grass volatile oil showed the greatest antifungal activity against Aspergillus flavus at concentrations of (0.006-0.03%) by inhibiting the fungal growth and aflatoxin production [39]. The antifungal abilities of Lemon, Mandarin, Grapefruit, and orange oils at a concentration of < 1% were reported against different species of Aspergillus and Penicillium molds [56]. Different EOs and their ingredients showed the most antifungal effects versus C. albicans drug-resistant biofilms by suppressing membrane ergosterol and varied signaling paths that prevent hyphal morphogenesis from the yeast [57]. ...
Medicinal herbs have an essential function in health and therapy. Bioactives of medicinal plants showed fewer side effects than manufactured drugs and their antioxidant properties are responsible for their different therapeutic properties. Essential oils (EOs) are an aromatic mixture of active components with a strong aroma obtained from aromatic plants. Natural EOs are composed of monoterpenes, sesquiterpenes, oxygenated monoterpenes, oxygenated sesquiterpenes, and phenolics. Because of their volatility, EOs are easily extracted from a variety of natural sources using the steam distillation method. Essential oils are one of the important therapeutic ingredients used in developing new therapeutic vehicles because of their anticancer, antiviral, antidiabetic, anticancer, antibacterial, antioxidant, and aromatherapy capabilities. This review includes different literature to survey the nature of essential oils, therapeutic potentials, and the key components of various essential oils.
... Mostly, they are consumed as aroma flavor in the food industry, including alcoholic and nonalcoholic beverages, marmalades, gelatins, sweets, soft drinks, ice creams, dairy products, jams, candies, and cakes [8][9][10]. Moreover, citrus essential oils exhibited significant importance due to their wide range of biological activities such as antimicrobial [11][12][13], antifungal [14], antioxidant [15], antidiabetic [16], antihyperlipidemic [17], anti-inflammatory, anti-allergic [18], anticancer [19], anxiolytic [20], and insecticidal activity [21]. ...
The mass-based metabolomic approach was implemented using GC-MS coupled with chemometric analysis to discriminate between the essential oil compositions of six cultivars of Citrus reticulata. The antiaging capability of the essential oils were investigated through measurement of their ability to inhibit the major enzymes hyaluronidase, collagenase, and amylase involved in aging. GC-MS analysis resulted in the identification of thirty-nine compounds including β-pinene, d-limonene, γ-terpinene, linalool, and dimethyl anthranilate as the main components. Multivariate analysis using principal component analysis (PCA) and hierarchal cluster analysis (HCA) successfully discriminated the cultivars into five main groups. In vitro antiaging activity showed that Kishu mandarin (Km) (2.19 ± 0.10, 465.9 ± 23.7, 0.31 ± 0.01 µg/mL), Cara mandarin (Cm) (3.22 ± 0.14, 592.1 ± 30.1, 0.66 ± 0.03 µg/mL), and Wm (8.43 ± 0.38, 695.2 ± 35.4, 0.79 ± 0.04%) had the highest inhibitory activity against hyaluronidase, collagenase, and amylase, respectively. Molecular docking studies on the major compounds validated the activities of the essential oils and suggested their possible mechanisms of action. Based on our result, certain cultivars of Citrus reticulata can be proposed as a promising candidate in antiaging skin care products.
... Due to these properties, there is the possibility of using jasmine EOs in the vapor phase for food preservation [39]. Natural additives are considered to contribute to both quality and safety in a wide variety of foods [40]. In recent times, there has been a growing interest in safe, organic and natural products. ...
The essential oil of Jasminum grandiflorum has very good biological activity. The present study aimed to analyze the chemical composition and biological activity of J. grandiflorum. The main constituents of J. grandiflorum essential oil were benzyl acetate (37%), benzyl benzoate (34.7%) and linalool (9.6%). The antioxidant activity was 58.47%, which corresponds to 220.93 TEAC. The antimicrobial activity was weak to moderate, with inhibition zones ranging from 2.33 to 5.33 mm. The lowest MIC value was against Candida glabrata. The antimicrobial activity of the vapor phase of the essential oil was significantly stronger than that of the contact application. Biofilm analysis using a MALDI-TOF MS Biotyper showed changes in the protein profile of Pseudomonas fluorescens that confirmed the inhibitory effect of J. grandiflorum. The insecticidal potential of the essential oil against Oxycarenus lavatera and Brassicogethes aeneus was also demonstrated. Due to the properties of J. grandiflorum essential oil, it could find application as a biofilm control agent for the shelf-life extension and storage of fruits and vegetables and as a possible insecticidal agent.
... They are also utilized as natural preservatives because of their wide range of biological activities, which include antioxidant and antimicrobial actions [1]. These strong biological activities are attributed to the presence of terpenes, flavonoids, carotenes, and coumarins [2]. Several studies have investigated the volatile makeup of various parts of Citrus species due to their significant economic importance. ...
Citrus essential oils are routinely adulterated because of the lack of regulations or reliable authentication methods. Unfortunately, the relatively simple chemical makeup and the tremendous price variations among Citrus varieties encouraged the interspecies adulteration of citrus oils. In this study, a sensitive UPLC-MS/MS method for the quantitation of 14 coumarins and furanocoumarins is developed and validated. This method was applied to screen the essential oils of 12 different Citrus species. This study, to our knowledge, represents the most comprehensive investigation of coumarin and furanocoumarin profiles across commercial-scale Citrus oils to date. Results show that the lowest amount was detected in calamansi oil. Expressed oil of Italian bergamot showed the highest furanocoumarin content and the highest level of any individual furanocoumarin (bergamottin). Notable differences were observed in the coumarin and furanocoumarin levels among oils of different crop varieties and origins within the same species. Potential correlations were observed between bergapten and xanthotoxin which matches with known biosynthetic pathways. We found patterns in furanocoumarin profiles that line up with known variations among the Citrus ancestral taxa. However, contrary to the literature, we also detected xanthotoxin in sweet orange and members of the mandarin taxon. Using multivariate analysis, we were able to divide the Citrus oils into 5 main groups and correlate them to the coumarin compositions.
... However, the inhibitory effect of these EOs on microscopic fungi was reported in the research of other authors. According to Viuda-Martos et al. [50], grapefruit EO was the best in terms of the growth reduction in Penicillium chrysogenum and Penicillium verrucosum when several citrus EOs were tested. Kujur et al. [51] showed significant protection of maize seeds against fungal infection after treatment by nano-encapsulated jasmine EO. ...
Essential oils (EOs) from aromatic plants seem to have the potential to control several fungal pathogens and food contaminants. Botrytis cinerea is the main strawberry fruit contaminant causing high losses during storage. Here, thirteen EOs applied in the vapor phase were evaluated for their potential to inhibit the growth of three different strains of B. cinerea isolated from strawberry fruits. Eight EOs (lemongrass, litsea, lavender, peppermint, mint, petitgrain, sage, and thyme) were able to completely inhibit the growth of B. cinerea for 7 days when applied at a concentration of 625 μL·L−1. Four EOs with the lowest minimal inhibition concentrations (thyme, peppermint, lemongrass, and litsea) have been tested on strawberry fruits intentionally inoculated by B. cinerea. All four EOs showed high inhibition at a concentration of 250 or 500 μL·L−1, but only peppermint EO was able to completely inhibit B. cinerea lesion development at a concentration of 125 μL·L−1. The sensory evaluation of strawberries treated by EOs at a concentration 125 μL·L−1 resulted in a statistically significant decrease in taste, aftertaste, aroma, and overall quality. Lemongrass and litsea EOs scored better than thyme and peppermint ones, thus forming two viable methods for B. cinerea suppression and the extension of packed strawberries’ shelf life.
... For example, C. x sinensis var. Valencia can inhibit Aspergillus flavus [40], Aspergillus niger, Penicillum chrysogenum, and Penicillum verrrucosum [41] and many other fungal species. Similarly, essential oil extracts for different varieties/cultivars of C. x sinensis have been proven to inhibit Lactobacillus curvatus L. sakei, Staphylococcus carnosus, S. xylosus, S. aureus, and B. subtillis, E. coli [42]. ...
Oranges and mandarins in Chongqing, China, are mostly processed for juice and their peels are wasted or landfilled. To add value, it is essential to utilize the peels and extract useful materials, such as citrus essential oils (CEOs). Here, we report the metabolome analysis of the peels of Citrus x sinensis (CS) and Citrus reticulata (CR). In total, 793 metabolites were detected in the CS and CR peels, of which 242 were differentially accumulated. The metabolites were grouped into 12 compound classes. Terpenoids were the highest accumulated class of compounds followed by phenols and alcohols in both fruit peels. CR peels were rich in all types of compounds, whereas CS showed a relatively lower content of the detected compounds. The highest accumulated compounds were β-phellandrene, beta-ocimene, 1,3,6-octatriene,3,7-dimethyl, and d-limonene. Overall, CR showed higher compound diversity than CS. The CS and CR peel extracts showed antibacterial effects against Escherichia coli, Staphylococcus aureus, and Bacillus subtilis. The peel extracts from CR and CS showed similar antibacterial effects against E. coli and B. subtilis, while CS peel extracts were more effective against S. aureus. Overall, our study concludes that both CS and CR peels should not be wasted owing to the CEOs and respective antibacterial activities.
... sinensis) [75]. Many of these monoterpenes have been shown to have potential in medicinal applications to reduce inflammation [76] and as natural antioxidants [77] with numerous antimicrobial and antifungal properties [78][79][80][81][82][83][84][85]. Our GC-MS analysis also detected carene in the EO of both tested citrus species, whereas carvone was found in C. reticulata only. ...
Aphids are serious pests of a wide range of agricultural crops, including pomegranates and grapevines. In addition, due to the negative environmental impacts of chemical insecticides, these pests are developing important resistance against aphicides. Therefore, one alternative method to control aphids is the use of essential oils (EO). The present study aimed to evaluate the insecticidal activity of Citrus aurantium and C. reticulata peel EO at different concentrations and with different exposure periods to pomegranate and grapevine aphids, Aphis punicae and A. illinoisensis via the topical application method under laboratory conditions. The results reveal that C. aurantium L. EO had greater toxicity against pomegranate and grapevine aphids, with LC50 of 0.37 and 0.82 μL/mL, respectively, at 48 h after application. The highest repellence effect was estimated for C. aurantium EO, at 2.5 μL/cm2, on A. punicae, with a value of 100% after an exposure time of 3 h, in contrast to the 88% repellence estimated for A. illinoisensis. The GC-MS investigation of both essential oils identified limonene, 3-carene, pinene, and p-cymene as active substances that could be attributed to the effects observed. Overall, our results offer a potential tool to control the two aphid species and could help in the development of integrated insect management in pomegranate and grapevine fields.
... niger, Trichoderma viride, Penicillium cyclopium). Viuda-Martos et al. (2008) [18] studied on complete mycelial inhibition of A. niger and A. flavor at a dosage of 0.94% lime, orange, mandarin and grapefruit EOs. They also reported high efficacy of grape EOs against Penicillium verrucosum and P chrysogenum, orange EO against A. niger and mandarin EO against A. flavus. ...
Since the era of the beginning of the eco-friendly pest management, various plant-based products showed promising actions, and amidst them, essential oils obtained from the leaves, barks, stems and different parts of the plants proved to be a boon towards effective control of various phytopathogens. Essential oils are odoriferous, volatile organic compounds, consisting of a broad spectrum of secondary plant metabolites. Besides their antibacterial, insecticidal, antimicrobial activities, they have enormous potential to reduce the damage due to phytopathogenic fungus. In about 3000 essential oils identified, only 300 found to be economical, and a narrow spectrum of them has been used to manage fungi. As for as their mode of action is concerned, in fungal pathogens, essential oils establish a membrane potential across the cell wall and disrupt ATP assembly, cell walls and their permeability, leading to cell wall damage. Moreover, improvement in formulations like nano and microemulsions and stabilizing formulations has led to a new paradigm towards efficient control of plant pathogenic fungi. Therefore, the present review depicts the involvement of various plant essential oils in fungicidal and fungistatic activities for controlling plant diseases and also emphasizes on the modes of action of various essential oil products and their formulations available. It also reveals the constraints regarding the essential oil formulations which we have to eliminate for further betterment in eco-friendly phytopathogenic fungi management.
... Generally, the antimicrobial efficacy of plants is due to diverse compounds such as phenolics, tannins, and alkaloids (Cowan 1999). Plant extracts can be used as food preservatives because they are referred to as GRAS (Generally Recognised As Safe) (Viuda-Martos et al. 2008) and, thanks to their safety, they have been used for this purpose since ancient times (Ullah et al. 2016). Plant extracts are still very important for processing safe and stable foods because foodborne illnesses still pose a threat even in industrialized countries, even though hygiene standards have steadily improved in recent decades (Newell et al. 2010). ...
In this study, almond skin of Prunus dulcis L. variety Casteltermini was characterised for its chemical composition and for the inhibitory activity towards food associated microorganisms belonging to pathogenic (Salmonella Enteritidis, Staphylococcus aureus, Escherichia coli and Listeria monocytgenes), spoilage (Pseudomonas poae and Brochotrix thermospacta) and useful (Levilactobacillus brevis and Saccharomyces cerevisiae) groups. The n-hexane extract of P. dulcis skin, investigated by GC-MS, evidenced linoleic, palmitic, and oleic acids as the main abundant compounds. Staphylococcus aureus ATCC 33862 growth was strongly limited by n-hexane extract and the minimum inhibition concentration (MIC) was 25.5 mg/mL. These results highlight the potential of almond by-products as food bio-preservatives as well as pharmaceutical products.
... Thus, when the concentration of pomelo peel EO was greater than 3.5 mg/mL, it exhibited a significant antifungal property. Experiments have demonstrated that PPEO can be used as a natural, pollutant-free pesticide [34,35]. ...
To verify the anti-fungus properties of the crop-harmful pseudocercospora, the essential oil (EO) of pomelo peel (PP) was extracted by a single factor combined with response surface optimization. Meanwhile, the composition and activity of EO were studied. The PP was squeezed by a screw extruder and pretreatmented by pectinase, then extracted by microwave-assisted steam distillation. The optimal conditions were as follows: pectinase dosage was 69.17 μmol/g, microwave power was 651.42 W and extraction time was 43.84 min. The dry weight (DW) yield of PPEO reached 14.63 mL/kg DW after BBD optimization. There were 23 compounds in the PPEO identified by GC-MS. Limonene, α-phrenbutene, and laurene in PPEO accounted for 79.31%, 4.72%, and 3.46%, respectively. In addition, the antifungal was effective when the concentration of PPEO was 3.5 mg/mL. Therefore, this study has guiding significance for the development of natural resources.
... The inhibitory percentage of EOs at different concentrations against A. flavus is described in Figure 4. The antifungal efficacy of orange peel EOs was dose-dependent manner which was compatible with earlier reported studies (Singh et al., 2010;Viuda-Martos et al., 2008). EOs at 10% was found to inactivate 10% of diameter of mycelial growth and nearly 70% of mycelial growth diameter of A. flavus was inhibited by 50% EOs. ...
The continuous fresh consumption or juice production of orange fruits (citrus nobilis) has discarded a vast number of orange peels, which has caused significant impacts on environmental issues. This study attempted to utilize discarded orange peels to extract essential oils (EOs) and evaluated their physiochemical properties, antibacterial, antifungal, and antioxidant activities. EOs were extracted via a distillation system using a Clevenger apparatus and gas chromatography - mass spectrometry analysis was employed to characterize their chemical components. The antibacterial and antifungal test were evaluated using a well diffusion method, and antioxidant activity was determined based on DPPH radicals scavenging effect and ferric reducing antioxidant power (FRAP). The obtained EOs with the yield of 3.29 ± 0.24% in which limonene was found to be the most abundant compound in the EOs (90.42%) followed by β-myrcene (4.7%) and α-pinene (1.22%). The result showed that Gram-positive bacterium (Bacillus cereus) was susceptible to the 50% EOs than Gram-negative bacterium (Escherichia coli) with respect to inhibitory zone diameter of 15.00 ± 0.58 mm and 11.33 ± 0.58 mm. The 50% EOs also inhibited nearly 70% of the mycelial growth of Aspergillus flavus as well as exhibiting antioxidant activity with IC50 values of 0.15 ± 0.01 mg/mL and 18.29 ± 0.13 mg/mL for DPPH and FRAP assay, respectively. The orange peel EOs could be a promising alternative to synthetic preservatives in food industry due to their antimicrobial and antifungal activity as well as their antioxidant activity.
... Ciprocin (Ciprofloxacin) is a fluoroquinolone antibiotic used to prevent bacterial infections such as bone and joint infections, intra-abdominal infections, certain types of infectious diarrhea, respiratory tract infections, skin infections, typhoid fever, urinary tract infections, etc. Results from the third disk diffusion test of the peel oil, represented in Table 3, show that the gram-positive S. aureus bacteria was more sensitive to the tested extracts than the other two gram-negative bacteria. Most studies have investigated the action of EOs against food spoilage microorganisms and food-borne pathogens and showed that these oils are slightly more active against the gram-positive than the gram-negative bacteria [46]. They studied the resistance against bacteria and represented the inhibition zones against the ciprofloxacin and erythromycin. ...
Citation: Plabon, M.E.A.; Mondal, S.C.; Or Rashid, M.M.; Chowdhury, M.K.; Saeid, A.; Althobaiti, F.; Dessok, E.S.; Rehmani, M.I.A.; Mustafa, S.K.; Islam, M.S. Chemical Composition and Anti-Microbial Activity of Hog Plum (Spondias mombin L.) Peel Oil Extracted from Different Regions of Tropical Climates. Horticulturae 2021, 7, 428. https://doi.org/10.3390/ horticulturae7110428 Academic Editors: Dasha Mihaylova and Aneta Popova
... Citrus essential oils are natural compounds with high concentration of limonene (LM) (> 90%) [19][20][21], thus presenting great potential for use in food preservation. In vitro studies have found limonene-enriched essential oils to present significant antimicrobial effects against common foodborne pathogens such as bacteria (Listeria monocytogenes, Staphylococcus aureus, Bacillus cereus, Escherichia coli, and Campylobacter jejuni) [22] and fungi (Aspergillus niger, Aspergillus flavus, Penicillium chrysogenum, and Penicillium verrucosum) [23]. Due to their hydrophobicity, terpenes have the ability to break and penetrate the lipid structure of the bacterial cell wall, causing protein denaturation and cell membrane destruction [24][25][26]. ...
Chitosan (CH) is an abundant and promising polysaccharide to produce biodegradable films due to its intrinsic properties, having its performance enhanced upon association with additives. In this context, limonene (LM), a terpene obtained from citrus peel oil, can be an excellent option for developing active food packaging. This study investigated the effect of the addition of LM at different concentrations (0.15%, 0.30%, and 0.45% w/w) on the structure, morphology, water vapor and light barrier, as well as mechanical, thermal, and antioxidant properties of chitosan films. Fourier transform infrared spec-troscopy, scanning electron microscopy, and X-ray diffraction results suggest that LM presented satisfactory incorporation into the biopolymer matrix, promoting a decrease in CH crystallinity, especially at the highest concentration. With increased concentrations of LM, tensile strength decreased from 23.27 to 13.30 MPa and elongation at break from 43.37 to 34.73%. LM incorporation had no effect on CH films thermal stability and water solubility, as well as on the equilibrium moisture content, which was maintained for all samples in water activity ranging from 0 to 95%. We also identified a type III water sorption isotherm behavior for all samples, and the Guggenheim-Anderson-Boer model provided the best fit. Finally, samples showed barrier to water vapor, water vapor transmission rate from 546.5 to 372.31 g m −2 day −1 , and to UV light. The results indicate that LM can be considered a promising additive for obtaining sustainable chitosan films for food preservation.
The required processes and steps for making bread include technological and innovative concepts. The current trend is the use of less toxic compounds and green processes. Besides lactic acid bacteria and yeast, other microorganisms with special properties, such as enzymes, new aroma and flavor, exopolysaccharides, and vitamins, among other compounds with beneficial properties, could be added to bread manufacture, improving bread quality and health effects for the consumers. Freeze drying, new encapsulation methods, cryoprotectants, spray drying, fluidized bed drying, and vacuum drying are used for probiotics and microorganism cultures that will be used as starters or biological additives in the fermentation. The same development is observed in the preservation methods, and studies with plant extracts and essential oils have been proposed and introduced, replacing chemical agents, such as propionate, within the clean-label bread formulations concept. Baking science is a growing research line incorporating innovative methods and biological additives.
Limiting postharvest losses of fruit from fungal attack is a major priority. While synthetic chemical fungicides are efficacious, the development of chemical resistance and consumer concerns are driving reinvestigation of natural antimicrobial agents. This review emphasizes the control of postharvest decay in citrus fruits with the use of some most common essential oils (EO), the efficacy of EOs in combating fungal infection in both in vitro and in vivo models, and the mode of action of EOs along with the potency of photochemical by-products that have also been investigated as antifungal agents.
The inclusion of plant components in meat products plays a substantial role in human nutrition, since such inclusions are a source of biologically active substances, promote digestion, and increase the nutritional composition of food products. The purpose of this paper is to review the latest studies on the use of plant components in the production of meat products and search for new ones that can be used as substitutes for artificial additives and meat raw materials. During the study, methods of analysis, comparison, and synthesis of papers of Ukrainian and foreign researchers located in scientometric databases, such as Web of Science, Scopus, and internet resources, are used. During the study of the literature, the main problems of meat products production are considered: spoilage of products during storage, increased content of nitrates and phosphates, development of pathogenic microflora. It is identified that the main centre of research is oxidative processes during the production and storage of finished meat products. It is identified that due to the high content of vitamins, phenolic compounds, and micro- and microelements, plant inclusions can act as inhibitors in such reactions. In the course of the study, it is determined that the most commonly used: peel, pulp, juices, and extracts of fruits and vegetables as plant inclusions. The main task of such inclusions is to replace synthetic inclusions with natural ones to increase the nutritional value and composition of food products. A comparative analysis of the nutritional value and chemical composition of fruits, vegetables, and their juices is conducted to select the most fitting plant inclusions that are common on the Ukrainian market. The indicators are summarised in the table. Replacing artificial antioxidants, preservatives, and other compounds with their natural counterparts is essential for modern manufacturing, as such technological solutions will help make food more functional, healthy, and increase the level of beneficial compounds in food.
This study was designed to determine the chemical composition, antioxidant, antimicrobial and insecticidal activities of essential oils of Thymus guyonii and Thymus algeriensis. The essential oils (EOs) were isolated by hydrodistillation and analyzed by GC and GC-MS. The leading components of the oils were p-cymene (15.8%), γ-terpinene (15.3%), thymol methyl ether (15.7%) and thymol (19.1%) for T. guyonii and α-pinene (4.4%), camphor (4.6%), bornyl acetate (5.4%) and terpinenyl actate (28.1%) for T. algeriensis. The antioxidant activity was evaluated in terms of scavenging of free radicals DPPH● and ABTS●+. The essential oil of T. guyonii was the most efficient in inhibiting free radicals but less active compared to synthetic antioxidants (Trolox and BHT). Also, T. guyonii essential oil showed the highest effectiveness against the rice weevil, Sitophilus oryzae L. (Coleoptera: Curculionidae) as well as against five microorganisms, two gram-positive (Staphylococcus aureus and Bacillus subtilis), one gram-negative (Escherichia coli), one yeast (Candida albicans) and one fungus (Aspergillus brasiliensis). The results suggest that the EO of T. guyonii could be considered in pharmaceutical and food fields as well as for preservation of stored products from S. oryzae damage.
Fruits are an abundant source of minerals and nutrients. High nutritional value and easy-to-consume property have increased its demand. In a way to fulfil this need, farmers have increased production, thus making it available for consumers in various regions. This distribution of fruits to various regions deals with many associated problems like deterioration and spoilage. In a way, the common practices that are being used are stored at low temperatures, preservation with chemicals, and many more. Recently, edible coating has emerged as a promising preservation technique to combat the above-mentioned problems. Edible coating stands for coating fruits with bioactive compounds which maintains the nutritional characteristics of fruit and also enhances the shelf life. The property of edible coating to control moisture loss, solute movement, gas exchange, and oxidation makes it most suitable to use. Preservation is uplifted by maintaining the nutritional and physicochemical properties of fruits with the effectiveness of essential oils. The essential oil contains antioxidant, antimicrobial, flavor, and probiotic properties. The utilization of essential oil in the edible coating has increased the property of coating. This review includes the process of extraction, potential benefits and applications of essential oils in food industry.
Citrus peels, which make up a significant portion of the entire fruit and are frequently rejected by nature and have a detrimental influence on the environment, are the focus of this study. These barks can be a highly valuable source of plant material. Three different citrus species C. limonum, C. reticulata, and C. Paradisi had their essential oils extracted from their peels(by-products) to create these citrus co-products (essential oil).The findings demonstrate how rich these citrus species are in essential oils. Limonene is the predominant component for the three, accounting for percentages of 56.3%, 76.5%, and 71.7% for C. paradisi, C. reticulata, and C. limonum, respectively, in their chemical composition as determined by GC/MS analysis. Their antioxidant activity was assessed using DPPH and validated using the FRAP assay. Furthermore, essential oils antimicrobial efficacy against five pathogenic strains: Escherichia coli, Enterococcus feacalis, Staphylococcus aureus, Candida albicans, and Sacharomyces cerevisiae, demonstrates significant antibacterial potential, particularly against Candida albicans.
Citrus fruits are one of the most valuable fruits grown and traded worldwide. It is also very important medically because it contains many components such as essential oils, minerals, trace elements, vitamins, flavonoids, carotenoids, alkaloids and coumarins. In addition to being consumed as a nutrient, it has also been used for aromatic, medicinal and cosmetic purposes since ancient times. Recent studies have shown that citrus species have many medical effects such as neuroprotective, antimicrobial, antifungal, antiviral, anti-inflammatory, antioxidant and anticancer. Therefore, it is thought that the compounds obtained from citrus fruits can be used in the treatment or prevention of various diseases. Today, interest in natural products and drugs prepared from natural products is increasing due to the serious side effects of synthetic drugs. This situation increases the importance of using citrus species for medicinal purposes. It is important to carry out more studies on this valuable plant and to expand its medicinal uses. In this context, the present study summarizes the available resources for examining the medicinal properties of different citrus varieties.
Family apiaceae, an important source of bioactive compounds, used for the treatment of many diseases since ages. Objective: Present study was conducted to explore active components of Cuminum Cyminum essential oil. Endeavor of the current study was to investigate the phytochemical contents and antibacterial assessment of essential oil against gram positive and negative bacteria. Method: Essential oil was extracted from seeds by hydrodistillation, dried and stored at -4oC. physical and chemical characterization was done. Antibacterial activity was also determined. Results: GC-MS analysis revealed major components cuminal 35%, γ-terpinene 32%, α-terpinene-al 7%, γ-terpinene 4.45%, daucene 4.3%, and trans-caryophyllene 5.342%, some trace components like myrcene 0.12%, 1-8 cineole, and γ-terpinene-7-al were also present. Conclusion: Chemical components (cuminal, turpentine, daucene, caryophyllene) present in cumin essential oil is responsible for its biological activities.
This paper explores the medicinal plants used by Coqueiros Community, Brazil, for the treatment of various ailments. We apply the structured interview and the participant observation, and the local knowledge was analyzed using quantitative measures applied to the general sampling to contribute to selection of promising species for biotechnological development. This community cited 105 species from 53 plant families. The most representative families were Fabaceae, Asteraceae, Anacardiaceae, Myrtaceae, Annonaceae, Apocynaceae, Lamiaceae and Rutaceae. Among the species mentioned in this survey, 66,7% of them occur in the Cerrado as native vegetation. The species considered the most important by interviewees are those with action forthe largest number of health problems. Thus, the statistical indexes used here, such as Relative Impotance (RI), Informant Consensus Factor (ICF) and Relative Frequency of Citation (RFC), contributed to identify these species. To conclude, from this research we have known the potential of the Brazilian vegetation for the development of biotechnology.
Khasi Mandarin (Citrus reticulata) is a unique fruit usually cultivated in high terrains regions of North-East India. They are different from ordinary orange in terms of its biochemical content (limonin, secondary metabolites), pharmacological activities, and physicochemical appearance (bright orange in color, hard peel, sweet aromatic juice). It has high nutritional values (NV) by enriching beta carotene as powerful antioxidant for scavenging free radicals, magnesium for blood pressure regulation, potassium for healthy cardio vascular functions, thiamin for energy conversion from food and vitamin C as immune enhancer. Despite its NV, limonin an aglycones and glycosides are largely available in Khasi Mandarin. Presence of this compound in Khasi Mandarin display numerous pharmacological activities like antimicrobial, antioxidant, anticancer, antidiabetic, anti-inflammatory, analgesic, antibacterial, anti-virus, liver protection and pesticidal. In this review, we discussed not only about the morphological aspects but detailed focused has been given on the nutritional value of Khasi mandarin, post-harvest disease and its management, metabolism and bioactive functions of the limonin as therapeutic applications.
Inflammatory bowel disease (IBD) is a chronic, relapsing immune-mediated disease that always leads to a progressive loss of intestinal function. Therefore, it is important to find potential therapeutic drugs. This study was conducted to elucidate the effect of Aurantii Fructus immaturus flavonoid extract (AFI, 8% neohesperidin, 10% naringin) on DSS-induced intestinal inflammation and the gut microbiome. To explore the mechanism of action by which AFI protects against intestinal inflammation, a total of 50 mice were randomly divided into 5 groups [CG (control group), MG (model group), AFI low dose, AFI middle dose, and AFI high dose] and received 2.5% DSS for 7 days. Then, mice in the AFI groups were orally administered different doses of AFI for 16 days. The results showed that, compared with the MG group, the food intake and body weight were increased in the AFI groups, but the water intake was lower. Additionally, AFI significantly alleviated DSS-induced colitis symptoms, including disease activity index (DAI), and colon pathological damage. The levels of IL-6, IL-1β and TNF-α in serum and colon tissue were significantly decreased. The diversity and abundance of the intestinal microbiota in the AFI group were decreased. The relative abundance of Bacteroidota was increased, and the relative abundance of Firmicutes was decreased. AFI plays an important role in alleviating DSS-induced intestinal inflammation and regulating Oscillospira, Prevotellaceae and Lachnospiraceae in the intestine at low, medium and high doses, respectively. This report is a pioneer in the assessment of AFI. This study not only demonstrated the anti-inflammatory activity of AFI but also identified the microbiota regulated by different concentrations of AFI.
In this study, the antimicrobial and antioxidant properties of various extracts obtained from the wastes of products (tea seed, tangerine peel, and orange peel) grown in Rize province of the Eastern Black Sea Region were determined. After the tea seeds, tangerine, and orange peel were dried at two different temperatures (60°C and 80°C), ethanol, warm water, and hot water extracts were prepared at different rates (5%, 10%, and 25%, respectively). The 2,2-d iphen yl-1-picri hydra zil radical scavenging method was used to determine the antioxidant properties of the extracts, and also the antioxidant values in terms of trolox were calculated. To determine the antimicrobial effect of the extracts, the efficiency of these extracts against Listeria monocytogenes, Escherichia coli, Salmonella enterica, and Staphylococcus aureus bacteria was investigated by using the disk diffusion method. When the 2,2-d iphen yl-1-picri hydra zil free radical removal potentials of the. (2022). Determination of antimicrobial and antioxidant activities of wastes (tea seed, orange, and mandarin peel) of some local product grown in Rize province.
The modern industrial application of lemon is significantly associated with their juicy materials obtained from the pulpy region, leaving behind a remarkable mass of by-products which are often discarded as waste. Several studies have shown that materials obtained from all handling and processing operations have substantial biological benefits based on their concomitant phytochemistry. In addition, these materials have been found suitable as raw substrates for the syntheses and manufacturing of various food-based additives. This research succinctly delineates these concepts and offers a comprehensive framework of its etymology, rich history, and overall pomology. More importantly, recently studied underlying biochemical mechanisms for the actions of lemon and its by-products for metabolic disorders such as diabetes, cancer and hypertension are technically delineated. These materials have been appraised as natural sources for nutraceuticals studied to be potential alternatives to typical synthetic pharmaceutics.
Background
Citrus sinensis L., commonly called sweet orange, fruit waste (peel, seed, and pulp) oils, are used as natural preservatives due to their broad spectrum of biological activities, including antimicrobial and antioxidant effects.
Objective
The aim of this study was to investigate the physicochemical properties, antioxidant and antimicrobial activities of sweet orange peel and pulp oils extracted using the solvent extraction method.
Methods
The oil extraction was done in the Soxhlet apparatus using petroleum ether as a solvent. Then, the physicochemical properties of the oil extracts were assessed based on the determination of oil yield, acid value, free fatty acid, and peroxide value. The antioxidant activity of the oil extract was evaluated based on 2,2-diphenyl-1-picrylhydrazyl (DPPH), and hydrogen peroxide free radical scavenging activity as well as ascorbic acid content.
Results
The results indicated that significantly higher antioxidant activities with respect to ascorbic acid (47.94%) and DPPH value 85.20% were recorded for sweet orange pulp/juice oil. Stronger antibacterial activity with a maximum zone of inhibition (10.67mm), minimum inhibitory concentration (MIC) of 0.25µg/ml, and minimum bactericidal concentration (MBC) of 0.25µg/ml were recorded for fruit pulp oil extract against Staphylococcus aureus . Stronger antifungal activity with a maximum zone of inhibition (9.67mm), MIC (0.25µg/ml), and minimum fungicidal concentration (MFC) of 0.50µg/ml were also observed for fruit pulp oil extract against Aspergillus versicolor .
Conclusion
C. sinensis fruit pulp oil was found to demonstrate stronger biological activities, including both antioxidant and antimicrobial potentials.
The need to replace conventional, usually single-use, packaging materials, so important for the future of resources and of the environment, has propelled research towards the development of packaging-based on biopolymers, fully biodegradable and even edible. The current study furthers the research on development of such films and tests the modification of the properties of the previously developed biopolymeric material, by adding 10, respectively 20% w/v essential oils of lemon, grapefruit, orange, cinnamon, clove, mint, ginger, eucalypt, and chamomile. Films with a thickness between 53 and 102 µm were obtained, with a roughness ranging between 147 and 366 nm. Most films had a water activity index significantly below what is required for microorganism growth, as low as 0.27, while all essential oils induced microbial growth reduction or 100% inhibition. Tested for the evaluation of physical, optical, microbiological or solubility properties, all the films with the addition of essential oil in the composition showed improved properties compared to the control sample.
Scientists are indeed fascinated by the pharmacologically important chemicals found in medicinal plants. Citrus fruits possess several curing agents for the prevention of diseases. Therefore, experimentswere carried out to test the antibacterial activity of methanolic extracts of peels from various locally available citrus fruits such as citron (Citrus medica), satkora (Citrus macroptera)and adajamir (Citrus assamensis) against Bacillus spp. and E. coliusing the disc diffusion method. Different physicochemical characteristics of fruit juice were also determined. Methanolic extract of satkora peel had the highest antibacterial activity of2.2 and 2.6 cm while adajamir had the lowest antibacterial activity of 1.7and 2.1 cm as ZOI against Bacillus spp. and E. coli, respectively. In the case of pH and total soluble solids (TSS), the three citrus varieties showed a small variation where satkora showed the highesttotal phenolic content (TPC), vitamin C content, and antioxidant activity than the others. Citrus fruits can be exploited as antibacterial and antioxidant ingredients in food and nutraceuticals, according to the findings of this study.
One of the aims of sustainable development goals (SDGs) i.e., responsible production and consumption can be achieved through waste valorization. Recent developments in utilization of citrus wastes for its value addition are discussed in this chapter. Information related to different citrus species discussed to evaluate their potential. For the industrial production of bioactive components from citrus waste, extraction options need to be explored. With the aim to reduce the negative environmental impacts of the production techniques, greener protocols are preferred. Thus, different extraction techniques presented with comparative yield potential and related advantages. There is need to create balance between cost of production and greener options to achieve sustainability goals. Application of green processes, e.g., supercritical fluid extraction, microwave- and ultrasound-assisted extractions, and their pros and cons are the central core of the chapter. Analytical techniques involved during the analysis is discussed for its intended purpose. Bioactivity spectrum of different components is elaborated with detail literature reviews. Finally, industrial applications especially in the field of health, food, cosmetics are also discussed for its fruitful utilization.
Family apiaceae, an important source of bioactive compounds, used for the treatment of many diseases since ages. Objective: Present study was conducted to explore active components of Cuminum Cyminum essential oil. Endeavor of the current study was to investigate the phytochemical contents and antibacterial assessment of essential oil against gram positive and negative bacteria. Method: Essential oil was extracted from seeds by hydrodistillation, dried and stored at -4oC. physical and chemical characterization was done. Antibacterial activity was also determined. Results: GC-MS analysis revealed major components cuminal 35%, γ-terpinene 32%, α-terpinene-al 7%, γ-terpinene 4.45%, daucene 4.3%, and trans-caryophyllene 5.342%, some trace components like myrcene 0.12%, 1-8 cineole, and γ-terpinene-7-al were also present. Conclusion: Chemical components (cuminal, turpentine, daucene, caryophyllene) present in cumin essential oil is responsible for its biological activities.
Citrus fruits are a natural source of antioxidants, due to its high content of polyphenols, flavonoids and terpenes. Recently the recognition of these biomolecules and their close relationship with human health has become a new area of study, in constantly growing. This review mentions to the recent scientific work realized with grapefruit (Citrus paradisi), doing emphasis particular on the importance of its components bioactives and biological activities in the medical, pharmaceutical, food and environmental areas. Besides, the waste generated mainly are peels and seed, rich in antioxidants and antimicrobial compounds that has not been exploited in its totality
The grapefruit is one of the most produced and consumed citrus fruits in the world, with an average annual production of 7 million tonnes. It has become increasingly popular, as it is considered a low-calorie fruit, rich in vitamins, minerals and many other valuable nutrients (flavonoids, carotenoids, essential oils…). Over half a million tonnes of grapefruit undergo processing, mainly into juice, which generates large amounts of wastes (peel, seeds, pulp and leftover membrane), since only 50% of the fruit is used in this manufacturing process. Today, these wastes are mostly discarded or used as cattle feed. These practices are unfortunate, as grapefruit processing wastes could be a source of high-added economic value. Indeed, they represent a source of dietary fibers, sugars, essential oils, flavonoids and carotenoids, valuable in various industrial sectors (energetic, agricultural, pharmaceutical, cosmetic and food). Thus, if grapefruit wastes can be used to produce biogas or bio-energy, to fertilize soil or as biosorbent in wastewater treatment, the recovery of their bioactive compounds (e.g. pectin, essential oils, carotenoids and flavonoids) could be also highly pertinent, as these compounds present a wide spectrum of technological properties and/or health benefits. Extraction processes usually adopted to recover those compounds use organic solvents, high temperatures and long extraction times, which are considered to generate a high environmental impact. As a result, in recent decades, different operating strategies have been developed (ultrasound-, microwave-, pulsed electric field-assisted extraction, and supercritical fluids), with the objective of increasing extraction yields while reducing use of resources. However, most of them have still economic or technological limitations that prevent their development in small and medium-sized enterprises. The present chapter expands on the aforementioned aspects of grapefruit wastes, with the objective of demonstrating their high re-use potential. This chapter presents the potential valorization of grapefruit wastes in food and non-food applications and offers a brief overview of the main emergent extraction technologies.
Citrus fruits are among the most spread fruits in the world and in the Mediterranean region particularly. Different trees that belong to the Citrus family produce lemon, lime, grapefruit, oranges, mandarins/tangerines, and sour oranges. The Mediterranean region is producing more than 20% of Citrus fruits worldwide production. Globally, the production of Citrus fruits exceeds 120 million tons yearly. Therefore, an important part of these fruits is transformed into juices and marmalades, and to yield likewise a significant amount of waste. All Citrus fruits contain diversity and high amounts of various bioactive molecules, and so do the wastes. These bioactive molecules belong to different families: terpenes, carotenoids, limonoids, polyphenols, phenolic acids, flavonoids, polymethoxy flavones, pectin, fibers, etc. Several studies revealed the multiple biological activities of these chemical components: antioxidant, anti-inflammatory, anti-diabetic, anti-Alzheimer, and anti-tumor against several cell lines. Also, functional properties have been associated with the chemical compounds as health promoters and disease-preventing agents for human beings. The obtained extracts from Citrus fruits bio-wastes can be used in food products, additives to food products, nutraceuticals, functional food, and pharmaceuticals. The generated wastes are also exploitable as feed and raw material for energy recovery. This chapter aims to provide an overview of the different Citrus bio-wastes components and their chemical composition, their bioactive compounds, their biological activities and functional properties, and their food, non-food, and industrial applications.KeywordsCitrus fruitsBy-productWastesFunctional foodIndustrial applications
Essential oils derived from plant materials are a mixture of compounds that exhibit antibacterial properties. Due to their distinct aroma, they also serve as a desirable natural additive for various food products, including dairy products. In this study, the essential oils of lemon peels, clove buds, and juniper berries were obtained by steam distillation and characterized using gas chromatography–mass spectrometry to determine their chemical compositions and effects on the viability of seven Bifidobacterium strains. Furthermore, the effect of essential oils on the viability of Bifidobacterium animalis subsp. lactis Bb-12 was investigated in cream samples during fermentation and after storage for 21 days at 6 °C. The fatty acid composition of fat extracted from essential oils containing sour cream samples and the volatile aroma compound profile of the sour cream samples were also determined chromatographically. Among the 120 compounds identified, monoterpene hydrocarbons were dominant in the essential oils of lemon peels (limonene and γ-terpinene) and juniper berries (sabinene and β-myrcene), while eugenol and eugenol acetate were abundant in the essential oil of clove buds. In addition to these compounds, butanoic and acetic acids were found in the tested sour cream samples. In turn, fat extracted from these samples was rich in saturated fatty acids, mainly palmitic acid. Among the tested strains of the genus Bifidobacterium, B. animalis subsp. lactis Bb-12 was the most sensitive to the essential oils of clove and juniper, as indicated by the larger growth inhibition zones. However, both the concentration and type of essential oils used had no effect on the number of cells of this strain present in the cream samples immediately after fermentation and after its 21-day storage, which suggests that the tested essential oils could be a natural additive to dairy products.
Increasing concerns toward food safety and public health have rendered the use of synthetic chemicals in agricultural environments unacceptable. A shift toward biologically safe approaches has been considered a preferred strategy within the food handling chain and has received increasing attention over the past years in managing undesirable microbial growth. Although several studies have looked at the mode of action of most antagonists, the manipulation of microbial communities in food safety has not been fully explored. Very little is known about the effect of microbial diversity and composition in developing a healthy environmental approach for pathogen management in the farm to fork continuum. In view of the progress made in recent years in metagenomic technologies, information generated should be used to develop a dynamic approach that will consider a comprehensive approach involving environmentally friendly strategies in dealing with food losses caused by microbes to ensure food safety. Thus, this review includes information on the latest biocontrol applications to suppress undesirable microbial growth and extend fresh produce shelf life along the farm to fork continuum. The role of recent trends related to the potential of microbiomes in food safety and quality is further discussed. The use of physical treatments against pathogen growth is also highlighted.
The present study describes the chemical composition, antibacterial and antifungal activities of essential oils from Bidens pilosa, a traditional medicinal plant widely distributed in the subtropics and tropics. The essential oils from the fresh leaves and flowers of B. pilosa were analyzed by GC–MS. Forty-four components were identified, of which β-caryophyllene (10.9% and 5.1%) and τ-cadinene (7.82% and 6.13%) were the main compounds in leaves and flowers, respectively. The oils and aqueous extracts of leaves and flowers were subjected to screening for their possible antioxidant activities by using 2,2′-diphenyl-1-picrylhydrazyl (DPPH) and β-carotene bleaching methods. In the former case, the essential oils from leaves and flowers were found to be superior to all aqueous extracts tested with an IC50 value of 47.5 and 49.7μg/ml, respectively, whereas all extracts and essential oils seemed to inhibit the oxidation of linoleic acid in the latter case. The oils from B. pilosa exerted significant antibacterial and antifungal activities against six bacteria and three fungal strains. The inhibitory activity of the flower essential oils in Gram-negative bacteria was significantly higher than in Gram-positive. Our findings demonstrate that the essential oils and aqueous extracts of B. pilosa possess antioxidant and antimicrobial activities that might be a natural potential source of preservative used in food and other allied industries.
This study was designed to examine the in vitro antimicrobial and antioxidant activities of the essential oil and various extracts (prepared by using solvents of varying polarity) of Salvia tomentosa (Miller). The essential oil was particularly found to possess strong antimicrobial activity while other non-polar extracts and subfractions showed moderate activities while polar extracts remained almost inactive. GC and GC/MS analyses of the oil resulted in the identification of 44 compounds, representing 97.7% of the oil; β-pinene (39.7%), α-pinene (10.9%) and camphor (9.7%) were the main components. The samples were also subjected to screening for their possible antioxidant activity by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and β-carotene-linoleic acid assays. In the first case, the free radical scavenging activity of aqueous methanol extract (MW) was superior to all other extracts (IC50=18.7 μg/ml). Polar extracts exhibited stronger activities than non-polar extracts. In the case of the linoleic acid system, oxidation of the linoleic acid was effectively inhibited by the polar subfraction of the MW extract, while the oil was less effective. The MW extract showed 90.6% inhibition, that is close to the synthetic antioxidant BHT.
The phytochemical composition and the antibacterial activity of the essential oils obtained from the aerial parts of two Lamiaceae species, winter savory (Satureja montana L.) and wild savory (Satureja cuneifolia Ten.) were evaluated. Gas chromatography-mass spectrometry (GC-MS) analysis of the isolated oils resulted in the identification of twenty compounds in the oil of S. montana representing 97% of the total oil and 25 compounds of S. cuneifolia, representing 80% of the total oil. Carvacrol was the major constituent of the S. montana oil (45.7%). Other important compounds were the monoterpenic hydrocarbons p-cymene, -terpinene and the oxygenated compounds carvacrol methyl ether, borneol and thymol. Conversely, the oil of S. cuneifolia contained a low percentage of carvacrol and thymol. The major constituents of wild savory oil were sesquiterpenes -cubebene (8.7%), spathulenol, -caryophyllene, followed by the monoterpenic hydrocarbons limonene and -pinene. The screening of the antimicrobial activities of essential coils were individually evalated against nine microorganisms, using a disc diffusion metod. The oil of S. montana exhibited greater antimicrobial activity than the oil of wild savory. Maximum activity of winter savory oil was observed against Escherichia coli, the methicillin-resistant Staphylococcus aureus and against yeast (Candida albicans). The essential oil of S. cuneifolia was also found to inhibit the growth of pathogens such as S. aureus and E. coli. A fungicidal activity against C. albicans and Saccharomyces cerevisiae was also found in both oils.
The effects of beta-pinene on yeast cells were studied. This terpene inhibited respiration with glucose or ethanol as the substrate. The inhibition depended on the ratio of the terpene to the amount of yeast cells; for a fixed concentration of pinene, inhibition decreased as the amount of yeast cells increased. Pinene also inhibited the pumping of protons and K+ transport, but this inhibition was more marked with with ethanol than with glucose as the substrate, indicating the mitochondrial localization of the inhibition. The studies on isolated mitochondria showed a series of effects, starting with the disappearance of the respiratory control and deenergization of the organelles and followed by an inhibition of respiration at higher concentrations of the terpene. The effect on respiration could be localized to the cytochrome b region of the electron transport chain. No effect could be detected on the activity of ATPase. The effects can be ascribed to a localization of pinene on membranes which was also accompanied by a decrease in the fluorescence polarization of diphenyl hexatriene, probably meaning an increase in the fluidity of the membrane, localized preferentially to the mitochondria.
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The essential oil of Melaleuca alternifolia (tea tree) has broad-spectrum antimicrobial activity. The mechanisms of action of tea tree oil and three of its components, 1,8-cineole, terpinen-4-ol, and alpha-terpineol, against Staphylococcus aureus ATCC 9144 were investigated. Treatment with these agents at their MICs and two times their MICs, particularly treatment with terpinen-4-ol and alpha-terpineol, reduced the viability of S. aureus. None of the agents caused lysis, as determined by measurement of the optical density at 620 nm, although cells became disproportionately sensitive to subsequent autolysis. Loss of 260-nm-absorbing material occurred after treatment with concentrations equivalent to the MIC, particularly after treatment with 1,8-cineole and alpha-terpineol. S. aureus organisms treated with tea tree oil or its components at the MIC or two times the MIC showed a significant loss of tolerance to NaCl. When the agents were tested at one-half the MIC, only 1,8-cineole significantly reduced the tolerance of S. aureus to NaCl. Electron microscopy of terpinen-4-ol-treated cells showed the formation of mesosomes and the loss of cytoplasmic contents. The predisposition to lysis, the loss of 260-nm-absorbing material, the loss of tolerance to NaCl, and the altered morphology seen by electron microscopy all suggest that tea tree oil and its components compromise the cytoplasmic membrane.
The essential oil from the gum of Pistachio (Pistacia vera L. (Anacardiaceae)) grown in Turkey was obtained by the hydro-distillation method, and its chemical composition was analyzed by GC and GC-MS. Moreover, the antimicrobial activities of the oil against the growth of 13 bacteria and 3 pathogenic yeasts were evaluated using the agar-disk diffusion and minimum inhibitory concentration (MIC) methods. The results showed that the essential oil contained about 89.67% monoterpenes, 8.1% oxygenated monoterpenes and 1.2% diterpenes. alpha-Pinene (75.6%), beta-pinene (9.5%), trans-verbenol (3.0%), camphene (1.4%), trans-pinocarveol (about 1.20%), and limonene (1.0%) were the major components. The antimicrobial results showed that the oil inhibited nine bacteria and all the yeasts studied, and the activities were considerably dependent upon concentration and its bioactive compounds such as carvacrol, camphene, and limonene. Moreover, the essential oil of the gum was found to be more effective yeastcide than Nystatin, synthetic yeastcide. Furthermore, the antibacterial activities of the oil were lower than those of standard antibiotics, ampicillin sodium, and streptomycine sulfate under the conditions studied.
Carvacrol is a component of several essential oils and has been shown to exert antimicrobial activity. The structural requirements for the activity of carvacrol were determined by comparison to structurally related (nonessential oil) compounds. Removal of the aliphatic ring substituents of carvacrol slightly decreased the antimicrobial activity. The effect of the hydroxyl group of carvacrol on activity could not be determined by simply comparing it to p-cymene, because this compound is immiscible with water; therefore, 2-amino-p-cymene, the amino analogue of carvacrol, which has a similar hydrophobicity and structural characteristics, was used. 2-Amino-p-cymene had similar membrane disruption and bacterial killing characteristics as carvacrol showing that, contrary to previous reports, the hydroxyl group of carvacrol itself is not essential for the antimicrobial activity. However, the observed 3-fold lower activity for 2-amino-p-cymene as compared to carvacrol indicates special features in the antimicrobial mode of action of carvacrol due to the hydroxyl group.
The antimicrobial activity of three Salvia species, i.e. S. santolinifolia, S. hydrangea and S. mirzayanii, essential oils were investigated. The essential oils were obtained from the aerial parts of plants and analyzed by GC-MS. The main constituents of aforementioned species were alpha-pinene (72.4%), beta-pinene (6.6%) and limonene (5.3%); beta-caryophyllene (25.1%), 1,8-cineol (15.2%) and caryophyllene oxide (11.5%); alpha-terpinenyl acetate (22.6%), 1,8-cineol (21.2%) and linalool (8.9%), respectively. Bioassays exhibited that the property of the oil of S. myrzayanii was superior to others. The antimicrobial activity of essential oil from Salvia species may well be due to the presence of synergy between six tested compounds (linalool, 1,8-cineol, alpha-pinene, beta-pinene, beta-caryophyllene and limonene) and other constituents of the oils with various degrees of antimicrobial activity. Among these, linalool and 1,8-cineol had the highest antimicrobial activity.
The essential oil from fresh and dried rhizomes of Hedychium coronarium on GC-MS analysis resulted in the identification of 44 and 38 constituents representing 93.91% and 95.41%, respectively. The major components of the essential oil from fresh and dried Hedychium coronarium rhizome were 1,8-cineole (41.42%, 37.44%), beta-pinene (10.39%, 17.4%) and alpha-terpineol (8.8%, 6.7%). The aromatic oil has antifungal as well as antibacterial effects. The antimicrobial activities of the essential oil were individually evaluated against four microorganisms, including two bacteria and two fungi. It was found that the antimicrobial activity was higher in the fresh sample than the dried. Both samples showed a better activity against Trichoderma sp. and Candida albicans than against the bacteria Bacillus subtilis and Pseudomonas aeruginosa.
Microbial transformations of cyclic hydrocarbons have received much attention during the past three decades. Interest in the degradation of environmental pollutants as well as in applications of microorganisms in the catalysis of chemical reactions has stimulated research in this area. The metabolic pathways of various aromatics, cycloalkanes, and terpenes in different microorganisms have been elucidated, and the genetics of several of these routes have been clarified. The toxicity of these compounds to microorganisms is very important in the microbial degradation of hydrocarbons, but not many researchers have studied the mechanism of this toxic action. In this review, we present general ideas derived from the various reports mentioning toxic effects. Most importantly, lipophilic hydrocarbons accumulate in the membrane lipid bilayer, affecting the structural and functional properties of these membranes. As a result of accumulated hydrocarbon molecules, the membrane loses its integrity, and an increase in permeability to protons and ions has been observed in several instances. Consequently, dissipation of the proton motive force and impairment of intracellular pH homeostasis occur. In addition to the effects of lipophilic compounds on the lipid part of the membrane, proteins embedded in the membrane are affected. The effects on the membrane-embedded proteins probably result to a large extent from changes in the lipid environment; however, direct effects of lipophilic compounds on membrane proteins have also been observed. Finally, the effectiveness of changes in membrane lipid composition, modification of outer membrane lipopolysaccharide, altered cell wall constituents, and active excretion systems in reducing the membrane concentrations of lipophilic compounds is discussed. Also, the adaptations (e.g., increase in lipid ordering, change in lipid/protein ratio) that compensate for the changes in membrane structure are treated.
The antifungal potential of essential oils of oregano (Origanum vulgare), thyme (Thymus vulgaris) and clove (Syzygium aromaticum) was determined. To establish this antifungal potential, two molds related to food spoilage, Aspergillus niger and Aspergillus flavus, were selected. The agar dilution method was employed for the determination of antifungal activities. The three essential oils analyzed presented inhibitory effects on both molds tested. Oregano essential oil showed the highest inhibition of mold growth, followed by clove and thyme. Aspergillus flavus was more sensitive to thyme essential oil than A. niger. Clove essential oil was a stronger inhibitor against A. niger than against A. flavus.
The essential oil isolated from plantlets of Thymus mastichina L. ssp. mastichina cultured in vitro was analyzed by GC and GC/MS. The oil was characterized by the richness of 1,8-cineole (55.5%) and linalool (24.5%). These results are in accordance with the chemical composition previously reported for this taxon. The oil showed antifungal activity against eight pathogenic fungi of die genus Fusarium with MICs and MFCs ranging from 1500 to 2100 μg/mL and from 2.0 to 2.4 mg/mL, respectively.
The solubility in water of essential oil constituents is directly related to their ability to penetrate the cell walls of a bacterium or fungus. The antimicrobial activity of essential oils is due to their solubility in the phospholipid bilayer of cell membranes. Terpenoids which are characterized by their lability have been found to interfere with the enzymatic reactions of energy metabolism.
The inhibitory effects of five essential oils (thyme, sage, nutmeg, eucaptus and cassia) against Alternaria alternata were tested at different concentrations (100–500ppm) in vitro. The cassia oil and thyme oil both exhibited antifungal activity against A. alternata. The cassia oil inhibited completely the growth of A. alternata at 300–500ppm. The thyme oil exhibited a lower degree of inhibition 62.0% at 500ppm. Spore germination and germ tube elongation of the pathogens in potato dextrose broth was strongly inhibited in the presence of 500ppm cassia oil. Irreversible inhibition of fungal growth could be caused by exposure to 300ppm and 400ppm cassia oil for 6 days and 500ppm cassia oil for 3 days. Cassia oil at 500ppm reduced the percentage of decayed tomatoes. The experiments on reducing natural decay development of tomatoes gave similar results. Therefore, essential oils could be an alternative to chemicals for control of postharvest phytopathogenic fungi on fruits or vegetables.
The young mature-green lemon fruit manifests a significantly lower level of postharvest decay as compared to the older yellow fruit. Inoculation with Penicillium digitatum Sacc. demonstrated that the resistance of young fruit to decay is related to a factor localized in the oil glands of the flavedo. The main antifungal compound of lemon flavedo was identified as the monoterpene aldehyde citral. The flavedo of green lemon contained 1.5-2 times higher levels of citral as compared to the yellow fruit. In parallel with citral decline, the flavedo extracts of yellow lemons exhibited an increased level of the monoterpene ester neryl acetate, which exerted practically no inhibitory activity against P. digitatum and, in concentrations below 500 ppm, even stimulated development of the pathogen. During long-term storage of lemon fruit, citral concentration decreased in parallel with the decline of antifungal activity in the peel and with an increase of decay incidence. It is suggested that the level of citral in the flavedo is related to the resistance of lemon fruit to postharvest decay.
The chemical composition of the essential oils obtained from stem/leaves and flowers/fruits of Ferula szovitsiana (Umbelliferae) were analysed by GC and GC–MS. The composition of the oils samples from stem/leaves and flower/fruits were compared: 51 and 47 compounds were identified in the essential oils of stem/leaves and flowers/fruits, representing 97.7% and 95.9% of the total oils, respectively. Both essential oils were rich in monoterpenes (66.7% and 62.1%, respectively) with neryl acetate as major component (33.0% and 41.5%, respectively). Other abundant compounds for stem/leaves oil were β-caryophyllene (8.9%), -pinene (8.0%), β-pinene (6.7%), bicyclogermacrene (4.5%), caryophyllene oxide (4.1%), limonene (4.6%) and -terpineol (3.2%). These values for flowers/fruits oil were bicyclogermacrene (9.0%), -pinene (5.5%), β-pinene (3.9%), -cadinene (3.5%), and calarene (3.2%). Furthermore, the oils were tested against Gram-positive, Gram-negative bacteria and two pathogenic fungi. It was found that oil from F. szovitsiana represents the most potent antimicrobial candidates with MIC of 1.25 mg/disk for Bacillus subtilis. Copyright © 2007 John Wiley & Sons, Ltd.
Thirty-two essential oils from plants were screened for inhibitory effects on 13 food-spoilage and industrial yeasts. Of these, essential oils of allspice, cinnamon, clove, garlic, onion, oregano, savory, and thyme were most inhibitory. Oils were subsequently tested for their effects on biomass production and pseudomycelium formation of eight genera of yeasts. Garlic oil was a potent inhibitor of yeast growth at concentrations as low as 25 ppm. The oils of onion, oregano and thyme were also strongly inhibitory. Essential oils (100 ppm) had no effect on pseudomycelium production by Candida lipolytica. However, all eight essential oils delayed pseudomycelium formation by Hansenula anomala, whereas six oils stimulated pseudomycelium production by Lodderomyces elongisporus. Cinnamon and clove oils were clearly stimulatory to pseudomycelium production by Saccharomyces cerevisiae.
Monoterpenes have antifungal activity on pathogenic fungi. The purpose of this study was to examine the effect of seven monoterpenes on phospholipid and sterol composition of Sclerotium cepivorum Berk as well as lipid peroxidation of mycelia and sclerotia development. Most of the monoterpenes increased the ergosterol content with a consequent diminution in the phospholipid/sterol ratio. This ratio was significantly decreased by the thymol treatment. When the fungus was grown in the presence of thymol or borneol, the saturated fatty acid content and the lipid peroxides were increased, concomitant with an increase of the sclerotial diameter. These results indicate that thymol and borneol may be promoting generation of lipid peroxides. Sclerotial differentiation was retarded mainly by camphor, 1,8-cineole, linalool and menthol.
Lemon (Citrus limon cv Fino) juice industry by-products were used to obtain high dietary fiber powder. The effect of processing variables (direct drying, and washing previous to drying) on functional properties, fiber content and type, microbial quality and physicochemical properties of the fiber were evaluated. The obtained fiber powder had good functional, microbial quality and favorable physicochemical characteristics to be used in food formulations. Processing conditions affected fiber composition and properties. Water holding capacity was enhanced by washing (7 g water/g non-washed fiber powder; 12.6 g water/g washed fiber powder) and slightly decreased by the reduction in fiber particle size. Oil holding capacity (6.7 g oil/g fiber powder) was not affected by those factors. Acid detergent and neutral detergent fibers were highest in powder from washed lemon residue (23.73 and 32.91%, respectively). aw was lowest in washed lemon fiber powder (0.13). Washing prevented fiber browning during drying as reflected in color parameters. Washing water rinsed green components. Drying was the responsible for the decrease of bacterial populations (approx. 90% reduction in microbial counts).
The antifungal activity of Thymus capitellatus oils on Candida, Aspergillus and dermatophyte strains were studied. The essential oils were obtained from the aerial parts of the plants by water distillation and analysed by GC and GC–MS. Three chemotypes were characterized: 1,8-cineole (47.5%), 1,8-cineole/borneol (28.8% and 19.5%, respectively) and 1,8-cineole/linalyl acetate/linalool (27.5%, 20.0% and 17.0%, respectively). The minimal inhibitory concentration (MIC) determined according to the NCCLS protocols (M27-A and M38-P) and the minimal lethal concentration (MLC) were used to evaluate the antifungal activity of the oils against Candida (seven clinical isolates and three ATCC type strains), Aspergillus (five clinical isolates, two CECT and two ATCC type strains) and five dermatophyte clinical fungi strains. The oils exhibited antifungal activity for the dermatophyte strains, with MIC values of 0.32–1.25 µ µ µ µ µl/ml; the chemotype 1,8-cineole/linalyl acetate/linalool proved to be more active. The highest antifungal activity of this oil can be associated with the contribution of the linalyl acetate. In the other hand, all samples showed low activity against Candida and Aspergillus strains.
The antimicrobial effects of essential oil isolated from Myrtus communis L. against Es-cherichia coli, Staphylococcus aureus, Streptococcus faecalis, Pseudomonas aeruginosa, Klebsiella pneumoniaee, Bacillus subtilis, Bacillus licheniformis, Candida albicans and Sac-charomyces cerevisiae were studied. The disc diffusion method was used to evaluate the zone of microbial growth inhibition at various concentrations of the oil. The minimal In-hibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) of the oil were determined. The oil was found to have an antimicrobial effect. Kinetics of microbial destruction exhibited that the oil could kill microorganisms within 45 minutes of expo-sure. GC and GC/MS analysis of the oil revealed 32 components. The major components were α-Pinene (29.4%), Limonene (21.2%), 1,8-Cineole (18%), Linalool (10.6%), Linalyl acetate (4.6%) and α-Terpineole (3.1%). The high monoterpenes hydrocarbons such as α-Pinene and Limonene seem to contribute to the strong antimicrobial activity of Myrtus communis L. Further research on antimicrobial effects of essential oils with a view to sub-stituting the common chemical compounds is promising. of Iran. 3 Institute for Research in Forests and Rangelands Km 15, Tehran-Karaj Highway, Tehran, Islamic Repub-lic of Iran. Email address: mrezaee@rifr-ac.org 4 Institute for Research in Forests and Rangelands, Km 15, Tehran-Karaj Highway, Tehran, Islamic Repub-lic of Iran.
The essential oil extracted from the epicarp of Citrus sinensis exhibited absolute fungitoxicity against the 10 post-harvest pathogens. GC–MS studies of the oil revealed the presence of
10 chemical constituents, of which limonene was found to be the major component (84.2%). The activity of the oil was tested
by the poisoned food technique (PF) and the volatile activity (VA) assay and the oils showed greater toxicity in the VA assay
than in the poisoned food assay. The nature of the toxicity was studied in the VA assay and it was observed that the oil was
fungicidal for the 10 pathogens in the 700ppm (mg/l) to 1000ppm range. The oil was extremely toxic for spore germination
and it was found that at 700ppm, spore germination was inhibited in the 10 test fungi out of the 12 tested. Treatment at
300ppm concentration exhibited 70–100% inhibition of spore germination in most of the fungi tested. Scanning electron microscopy
(SEM) was done to study the mode of action of the oil in Aspergillus niger and it was observed that treatment with the oil leads to distortion and thinning of the hyphal wall and the reduction in
hyphal diameter and absence of conidiophores.
The aerial parts of two endemic Pimpinella [Pimpinella anisetum Boiss. & Ball. and Pimpinella flabellifolia (Boiss.) Benth. ex Drude] were hydro-distilled to produce oils in the yields of 2.07% (v/w) and 2.61% (v/w), respectively. The oils were analysed by GC and GC/MS. Twenty-one and nineteen components were identified, representing 99.5% and 99.7% of the oils, respectively. The main compounds of P. anisetum were (E)-anethole (82.8%) and methyl chavicol (14.5%), whereas limonene (47.0%), (E)-anethole (37.9%) and α-pinene (6.0%) were the major constituents of P. flabellifolia. The oils were screened for their possible antioxidant activities by two complementary test systems, namely DPPH free radical-scavenging and β-carotene/linoleic acid systems. In the first case, P. anisetum oil exerted greater antioxidant activity than that of P. flabellifolia oil with an IC50 value of 5.62 ± 1.34 μg/ml. In the β-carotene/linoleic acid test system, the oil of P. anisetum was superior to P. flabellifolia with 70.5% ± 2.86 inhibition rate. Essential oils of the plants studied here were also screened for their antimicrobial activities against six bacteria and two fungi. The oils showed moderate antimicrobial activity against all microorganisms tested.
Antifungal activity of essential oils of thyme, summer savory and clove were evaluated in culture medium and tomato paste. Aspergillus flavus were inoculated in Sabouraud Dextrose Broth and tomato paste and then 0, 50, 200, 350 and 500 ppm of essential oils were added to each sample and then kept at 25 ± 0.5 °C for 2 months. Results showed that all essential oils could inhibit the growth of A. flavus and the thyme oil and summer savory, showed the strongest inhibition at 350 ppm and 500 ppm, respectively. Taste panel evaluations were carried out in a tomato ketchup base, and the percent of inhibition of each essential oil in tomato paste was lower than culture medium. Taste panel was carried out and sample with 500 ppm thyme oil was accepted by panelists.
This study examined the effect of volatile components of citrus fruit essential oils on P. digitatum and P. italicum growth. The hydrodistilled essential oils of orange (Citrus sinensis cvv. “Washington navel”, “Sanguinello”, “Tarocco”, “Moro”, “Valencia late”, and “Ovale”), bitter (sour) orange (C. aurantium), mandarin (C. deliciosa cv. “Avana”), grapefruit (C. paradisi cvv. “Marsh seedless” and “Red Blush”), citrange (C. sinensis x Poncirus trifoliata cvv. “Carrizo” and “Troyer”), and lemon (C. limon cv. “Femminello”, collected in three periods), were characterized by a combination of GC and GC/MS analyses. The antifungal efficacy of the oils was then examined at progressively reduced rates. Findings showed a positive correlation between monoterpenes other than limonene and sesquiterpene content of the oils and the pathogen fungi inhibition. The best results were shown by the citrange oils, whose chemical composition is reported for the first time, and lemon. Furthermore P. digitatum was found to be more sensitive to the inhibitory action of the oils.
The antioxidant and antibacterial effect of rosemary, orange and lemon extracts was investigated in cooked Swedish-style meatballs. Activity in a lard system was established for all the extracts and further determination of the development of rancidity as thiobarbituric acid reactive substances consistently showed that about 50% of the rancidity can be controlled by the citrus preparations. Two of the rosemary extracts (water soluble and oil soluble) were more effective with practically complete elimination of rancidity (TBA values) after a period of 12 days. Rosemary extract activity against lactic acid bacteria and Listeria but not Brochothrix thermosphacta was demonstrated in an agar diffusion test, but in the product only lactic acid bacteria counts were slightly reduced. Sensory analysis results, particularly aroma and acceptability scores, indicated the significant advantages in using rosemary and citrus extracts in rancidity-susceptible meat products.
Microbial transformations of cyclic hydrocarbons have received much attention during the past three decades. Interest in the degradation of environmental pollutants as well as in applications of microorganisms in the catalysis of chemical reactions has stimulated research in this area. The metabolic pathways of various aromatics, cycloalkanes, and terpenes in different microorganisms have been elucidated, and the genetics of several of these routes have been clarified. The toxicity of these compounds to microorganisms is very important in the microbial degradation of hydrocarbons, but not many researchers have studied the mechanism of this toxic action. In this review, we present general ideas derived from the various reports mentioning toxic effects. Most importantly, lipophilic hydrocarbons accumulate in the membrane lipid bilayer, affecting the structural and functional properties of these membranes. As a result of accumulated hydrocarbon molecules, the membrane loses its integrity, and an increase in permeability to protons and ions has been observed in several instances. Consequently, dissipation of the proton motive force and impairment of intracellular pH homeostasis occur. In addition to the effects of lipophilic compounds on the lipid part of the membrane, proteins embedded in the membrane are affected. The effects on the membrane-embedded proteins probably result to a large extent from changes in the lipid environment; however, direct effects of lipophilic compounds on membrane proteins have also been observed. Finally, the effectiveness of changes in membrane lipid composition, modification of outer membrane lipopolysaccharide, altered cell wall constituents, and active excretion systems in reducing the membrane concentrations of lipophilic compounds is discussed. Also, the adaptations (e.g., increase in lipid ordering, change in lipid/protein ratio) that compensate for the changes in membrane structure are treated.
The isolated essential oils from seven air-dried plant species were analyzed by gas chromatography-mass spectrometry (GC-MS). Thymus vulgaris (thyme), Origanum vulgare (oregano), and Origanumdictamus (dictamus) essential oils were found to be rich in phenolic compounds representing 65.8, 71.1, and 78.0% of the total oil, respectively. Origanum majorana (marjoram) oil was constituted of hydrocarbons (42.1%), alcohols (24.3%), and phenols (14.2%). The essential oil from Lavandula angustifolia Mill. (lavender) was characterized by the presence of alcohols (58.8%) and esters (32.7%). Ethers predominated in Rosmarinus officinalis (rosemary) and Salvia fruticosa (sage) essential oils, constituting 88.9 and 78.0%, respectively. The radial growth, conidial germination, and production of Penicillium digitatum were inhibited completely by oregano, thyme, dictamus, and marjoram essential oils at relatively low concentrations (250-400 microg/mL). Lavender, rosemary, and sage essential oils presented less inhibitory effect on the radial growth and conidial germination of P. digitatum. Conidial production of P. digitatum was not affected by the above oils at concentrations up to 1000 microg/mL. Apart from oregano oil, all essential oils were more effective in the inhibition of conidial germination than of radial growth. The monoterpene components, which participate in essential oils in different compositions, seem to have more than an additive effect in fungal inhibition.
The high-speed GC separation and MS characterization of lime oil and lemon oil samples using programmable column selectivity and time-of-flight mass spectrometry is described. The volatile essential oils are separated on a series-coupled (tandem) column ensemble consisting of a polar trifluoropropylmethyl polysiloxane column and a nonpolar 5% phenyl dimethyl polysiloxane column. Both columns are 7 m long. A 50 degrees C/min linear temperature ramp from 50 to 200 degrees C is used, giving an analysis time of approximately 2.5 min. A time-of-flight MS with time array detection and automated peak finding and characterization software was used to identify 50 components in lime oil samples and 25 components in lemon oil samples. Despite numerous cases of extensive peak overlap, spectral deconvolution software was very successful in the characterization of most overlapping peaks. For cases where a more complete chromatographic separation is desirable, the tandem column ensemble is operated in the first-column stop-flow mode to enhance the separation of selected overlapping clusters of peaks. A valve between the junction point of the tandem column ensemble and a source of carrier gas at the GC inlet pressure is opened for 2-5-s intervals to stop the flow of carrier gas in the first column. This is used to increase the separation of target component groups that overlap in the ensemble chromatogram without first-column stop-flow operation. This procedure is used to isolate the peak for limonene, the largest peak in the analytical-ion chromatogram of both the lime and lemon oil samples.
The essential oil of leaves and peel from the Cretan variety Zambetakis (Citrus limon) was obtained by steam distillation with a Clevenger apparatus. The essential oil was subjected to GC-MS analysis, and 35 substances were identified. The main component in both essential oils was limonene. beta-Pinene, myrcene, neral, geranial, neryl acetate, geranyl acetate, and beta-caryophyllene have been identified in the leaf oil. The peel oil contained gamma-terpinene, beta-pinene, myrcene, neral, and geranial. The quantification of volatile substances was based on the internal standard method, using octyl acetate as internal standard, and expressed in milligrams per kilogram of the essential oil. The high contents of neral and geranial were indicative of the high quality of both essential oils. The aroma profile and quantitative variations among the essential oil components were measured at six different time intervals over a period of greater than one year. Differences between the components of lemon leaves and peel were observed.
The chemical composition of the essential oil of the Sardinian dwarf curry plant [Helichrysum italicum G. Don ssp. microphyllum (Willd) Nym] was studied. Genetic analysis suggested the presence of two chemotypes; morphological and chemical differences confirmed the presence of two chemotypes (A and B). The maximum yields were 0.18 and 0.04% (v/w) for flowering tops and stems, respectively. The concentrations of nerol and its esters (acetate and propionate), limonene, and linalool reach their highest values during the flowering stage both in flowers and in stems. Besides the essential oil, type B showed an interesting antifungal activity.
Essential oils from different plant parts are known for their antimicrobial activity but the antifungal effects of essential oil from Citrus sinensis (L.) Osbeck epicarp on growth and morphogenesis of Aspergillus niger has not been observed so far. The mycelial growth was inhibited at 2.5 and 3.0 microg/ml of oil in Potato Dextrose Broth and Agar medium, respectively. These concentrations were fungicidal under the test conditions. The fungitoxicity of oil did not change even at exposure to 100 degrees C and autoclaving. The main changes observed under light and scanning electron microscopy after oil treatment were loss of cytoplasm in fungal hyphae, and budding of hyphal tip. The hyphal wall and its diameter became markedly thinner, distorted and resulted in cell wall disruption. The flattened and empty hyphal tips bifurcated into bud like structures. GC-MS studies of the oil revealed the presence of 10 chemical constituents. Limonene has been found to be major component (84.2%).
The present article reports the antimicrobial efficacy of four monoterpenes (thymol, carvacrol, p-cymene, and gamma-terpinene) against the Gram-positive bacterium Staphylococcus aureus and the Gram-negative bacterium Escherichia coli. For a better understanding of their mechanism of action, the damage caused by these four monoterpenes on biomembranes was evaluated by monitoring the release, following exposure to the compounds under study, of the water-soluble fluorescent marker carboxyfluorescein (CF) from large unilamellar vesicles (LUVs) with different lipidic composition (phosphatidylcholine, PC, phosphatidylcholine/phosphatidylserine, PC/PS, 9:1; phosphatidylcholine/stearylamine, PC/SA, 9:1). Furthermore, the interaction of these terpenes with dimyristoylphosphatidylcholine multilamellar vesicles as model membranes was monitored by means of differential scanning calorimetry (DSC) technique. Finally, the results were related also with the relative lipophilicity and water solubility of the compounds examined. We observed that thymol is considerably more toxic against S. aureus than the other three terpenes, while carvacrol and p-cymene are the most inhibitory against E. coli. Thymol and carvacrol, but not gamma-terpinene and p-cymene, caused a concentration-dependent CF leakage from all kinds of LUVs employed; in particular, thymol was more effective on PC and PC/SA LUVS than on PC/PS vesicles, while carvacrol challenge evoked a CF leakage from PC/PS LUVs similar to that induced from PC/SA LUVs, and lower than that measured with PC vesicles. Concerning DSC experiments, these four terpenes caused a decrease in Tm and (especially carvacrol and p-cymene) DeltaH values, very likely acting as substitutional impurities. Taken together, our findings lead us to speculate that the antimicrobial effect of thymol, carvacrol, p-cymene, and gamma-terpinene may result, partially at least, from a gross perturbation of the lipidic fraction of the plasmic membrane of the microorganism. In addition to being related to the physicochemical characteristics of the compounds (such as lipophilicity and water solubility), this effect seems to be dependent on the lipidic composition and net surface charge of the microbic membranes. Furthermore, the compounds might cross the cell membranes, thus penetrating into the interior of the cell and interacting with intracellular sites critical for antibacterial activity.
Phenols and chelators
- Kabara
Kabara, J. J. (1991). Phenols and chelators. In N. J. Russell & G. W.
Gould (Eds.), Food preservatives (pp. 200-214). Glasgow: Blackie.
Epicarp of Citrus sinensis: A potential source of natural pesticides
- A C Shukla
- S K Shahi
- A Dixit
Shukla, A. C., Shahi, S. K., & Dixit, A. (2000). Epicarp of Citrus sinensis:
A potential source of natural pesticides. Indian Phytopathology, 53,
468-471.
Natural antimicrobials from plants