This study provides unique empirical evidence regarding a growing concern internationally: weight discrimination in the workplace. Using survey data from a national sample of 2838 American adults, it responds to Puhl and Brownell’s [Puhl, R., & Brownell, K. D. (2001). Bias, discrimination, and obesity. Obesity Research, 9, 788–805] call for additional research investigating the prevalence of discriminatory experience among overweight employees, and to their more specific call for research that takes sex and race into account when examining weight discrimination. The results indicate that women are over 16 times more likely than men to perceive employment related discrimination and identify weight as the basis for their discriminatory experience. In addition, overweight respondents were 12 times more likely than normal weight respondents to report weight-related employment discrimination, obese 37 times more likely, and severely obese more than 100 times more likely. The implications of the study’s findings for organizations, policy makers, overweight employees, and career counselors are discussed, and future research directions suggested.