V. Dimov and G Georgiev
Ram Semen Prostaglandin Concentration and its Effect on
J ANIM SCI
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1054 DIMOV AND GEORGIEV
TABLE 6. EFFECT ON FERTILITY UPON ADDITION OF PGE a TO THE
SEMEN OF TWO RAMS
of PGE 2
On the rest
of PGE (tag/ml)
in the semen
aNumber of animals (in brackets).
with the lowest concentration of prostaglandins
in the semen. For example, the addition of 50
/.tg prostaglandin E2 to one dose of diluted
semen for insemination from ram #4173 did
not change the fertility (table 6). The same
amount of prostaglandin E2 increased the fertil-
ity from 37.8 to 91% for ram #246. The con-
centration of prostaglandins in the semen of
these two rams is shown in table 2 (analyses
no. 11 and 12, respectively). It is obvious that
together with the number of spermatozoa and
their motility there are some other factors in-
fluencing fertilization. Our results suggest that
primary dienoic prostaglandins may stimulate
this process. It is also possible by adding pros-
taglandins to the media used to extend semen
to allow further dilution of semen without
lowering the fertility rate. According to How-
kins (1968) the fertility of oligospermic semen
of man is correlated with the concentration of
prostaglandins. It is possible to have the same
relationship in the ram semen. The concentra-
tion of primary prostaglandins in the semen of
man and ram is much higher than in other in-
vestigated species. It is tempting to speculate
that in these two species prostaglandins play a
specific role in the process of fertilization. Our
attempts to increase fertility of bulls by addi-
tion of prostaglandins to the semen were unsuc-
cessful (V. Dimov, unpublished results). It is
necessary to investigate more precisely the in-
fluence of different molecular species of pros-
taglandins (or a combination of them) on fertil-
ity. Since more than 85% of the ewes in Bulgar-
ia are inseminated artificially and fertility is
about 60 to 65% at the first insemination, this
procedure could permit shortening of the
mating season of ewes.
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