Amperometric biosensor for lactate analysis in wine and must during fermentation

Claude Bernard University Lyon 1, Villeurbanne, France
Materials Science and Engineering C (Impact Factor: 3.09). 07/2008; 28(5-6):943-948. DOI: 10.1016/j.msec.2007.10.038
Source: OAI


A lactate oxidase-based amperometric biosensor is designed for lactate determination. Two methods of lactate oxidase immobilization on the surface of commercial SensLab platinum printing electrodes are compared. The sensor with lactate oxidase immobilized by physical adsorption in Resydrol polymer is shown to have both narrower dynamic range (0.004–0.5 mМ lactate) and higher sensitivity (320 nA/mM) as compared with that immobilized in poly(3,4-ethylenedioxythiophene) by electrochemical polymerization (0.05–1.6 mM and 60 nA/mM respectively). The operational stability of the biosensors developed is studied; the immobilization method is shown to be of no influence. The lactate content in wine and in wine material during fermentation is analyzed. The data obtained by amperometric lactate biosensor correlated with those of standard chromatography. The biosensor developed can be used in food industry for control and optimization of process of wine fermentation as well as for control of wine quality.

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Available from: Alexei P Soldatkin
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    • "An important biocompound that is in need of reliable monitoring is l-lactate. Biosensor applications for the determination of l-lactate range from biotechnology (food quality control [13], cell culture monitoring [14]) to monitoring different aspects of human health [15]. Some previous examples for the amperometric biosensing of l-lactate have been described using l-lactate oxidases (FMNdependent ) and l-lactate dehydrogenases (NAD + -dependent) from various natural sources. "
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    • "Electrochemical polymerization is of remarkable interest due to its technological facilities. It enables to select and maintain dimensions , shape and thickness of the matrix and to provide exact control over precipitation [18] [19]. Poly(3,4-ethylenedioxythiophene) (PEDT) is one of polythiophenes , conductive polymers with novel promising properties. "
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    • "Electrochemical polymerization is of remarkable interest due to its technological facilities. It enables to select and maintain dimensions , shape and thickness of the matrix and to provide exact control over precipitation [18] [19]. "

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