Stimulation of collagen synthesis in fibroblast cultures by the tripeptide-copper complex Gly-His-Lys-Cu2+

French National Centre for Scientific Research, Lutetia Parisorum, Île-de-France, France
FEBS Letters (Impact Factor: 3.17). 11/1988; 238(2):343-346. DOI: 10.1016/0014-5793(88)80509-X
Source: PubMed


Glycyl-L-histidyl-L-lysine (GHK) is a tripeptide with affinity for copper(II) ions and was isolated from human plasma. This peptide appears to play a physiological role in wound healing. We report the stimulating effect of GHK-Cu on collagen synthesis by fibroblasts. The stimulation began between 10−12 and 10−11 M, maximized at 10−9 M, and was independent of any change in cell number. The presence of a GHK triplet in the α2(I) chain of type I collagen suggests that the tripeptide might be liberated by proteases at the site of a wound and exert in situ healing effects.

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    • "Subsequent studies directed by Maquart et al. (France) demonstrated that GHK-Cu at a very low, nontoxic concentration (1–10 nanomolar) stimulated both the synthesis and breakdown of collagen and glycosaminoglycans [55, 56]. It modulated an expression of both metalloproteinases and their inhibitors (TIMP-1 and TIMP-2), improving wound healing and facilitating skin remodeling processes [57]. "
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