Determination of acetaldehyde in human blood by a gas chromatographie method with negligible artefactual acetaldehyde formation

Department of Clinical Chemistry, Bispebjerg Hospital, DK-2400 Copenhagen NV Denmark
Clinica Chimica Acta (Impact Factor: 2.82). 11/1981; 116(3):389-395. DOI: 10.1016/0009-8981(81)90058-9


A method is described for determination of acetaldehyde in blood by head space gas chromatography.The method utilizes sodium nitrite-sulfosalicylic acid as an inhibitor of the ethanol oxidizing systems by means of which the interference of ethanol is reduced considerably. The detection limit was 0.4 μmol/l, the recovery 101.5 ± 5.2% and the coefficient of variation was 7.8% (1.5 μmol/l acetaldehyde). There was no disappearance of acetaldehyde if the head space vials were kept at −20°C for 24 h. In the comparison study with the semicarbazide method our results were 0.7–4.1 μmol/l lower. The values for acetaldehyde in blood after ethanol ingestion (0.5 g/kg) by volunteers were 0.5–1.3 μmol/l.

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    ABSTRACT: A comparison is made of four previously described methods for the preparation of blood for acetaldehyde (AcH) assay in the rat. The spontaneous formation of AcH which occurs during the treatment of blood containing ethanol and the recovery of known amounts of AcH added to the blood were studied. The methods using sodium nitrite-sulfosalicylic acid or perchloric acid (PCA) in saline gave low levels of spontaneous formation (1 to 2 microM AcH for 48 mM ethanol). In the recovery studies it was seen that semicarbazide does not allow displacement of all the AcH; treatment of the blood with the reactant sodium nitrite-sulfosalycilic acid and use of the hemolysis method gave levels of recovery lower than 50%. Only treatment of the blood with perchloric acid in NaCl allowed all the AcH added to the blood to be recovered. In vivo, PCA in saline releases the AcH which was seen to remain bound in the red blood cells with the semicarbazide method. So the recommended procedure for accurate assay of blood AcH in the rat is cold deproteinization in PCA/saline before head-space gas chromatography. The levels of in vivo blood AcH (4.1 +/- 0.33 microM) obtained in the rat using this method for a blood alcohol concentration of 52 mM are lower than those previously described in the literature.
    No preview · Article · Dec 1983 · Analytical Biochemistry
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    ABSTRACT: Determination of acetaldehyde in human blood by the semicarbazide method has been studied. An artefactual production of acetaldehyde from ethanol in blood and plasma of about 20 mumol/l was observed at concentrations of ethanol of about 60 mmol/l. This artefact was reduced to less than 1 mumol/l after addition of thiourea. The presumably non-enzymatic production of acetaldehyde from ethanol was demonstrated by the release of 3H from (1R)-[3H]ethanol added to the blood immediately after sampling. The results demonstrate that oxidation of ethanol is the major cause of the artefactual acetaldehyde formation. In human volunteers, metabolising ethanol, very low concentrations of acetaldehyde were found by the modified method, which includes thiourea.
    No preview · Article · Jan 1984 · Clinica Chimica Acta
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    ABSTRACT: Alcoholism represents one of the most serious health problems in modern industrialized society. In spite of persistent research efforts and preventive initiatives the main problems concerning etiology remain unsolved, while the consumption of alcohol in a society such as Denmark still increases.
    No preview · Chapter · Jan 1984
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