Amperometric biosensor based on carbon nanotubes coated with polyaniline/dendrimer-encapsulated Pt nanoparticles for glucose detection

Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237, China
Materials Science and Engineering C (Impact Factor: 3.09). 05/2009; 29(4):1306-1310. DOI: 10.1016/j.msec.2008.10.031


A novel amperometric glucose biosensor based on the nanocomposites of multi-wall carbon nanotubes (CNT) coated with polyaniline (PANI) and dendrimer-encapsulated Pt nanoparticles (Pt-DENs) is prepared. CNT coated with protonated PANI is in situ synthesized and Pt-DENs is absorbed on PANI/CNT composite surface by self-assembly method. Then Glucose oxidase (GOx) is crosslink-immobilizated onto Pt-DENs/PANI/CNT composite film. The results show that the fabricated GOx/Pt-DENs/PANI/CNT electrode exhibits excellent response performance to glucose, such as low detection limit (0.5 µM), wide linear range (1 µM–12 mM), short response time (about 5 s), high sensitivity (42.0 µA mM− 1 cm− 2) and stability (83% remains after 3 weeks).

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    • "It was much wider than that of the ZnO/MWCNT/GOD electrode (6.67 to 1,290 μM) [39], Ag polydopamine@CNT/Nafion/GOD electrode (50 to 1,100 μM) [40], and GR quantum dot/GOD electrode (5 to 1,270 μM) [30]. The detection limit was estimated to be 0.3 μM (based on S/N = 3) for glucose, which was lower than 20 μM for MWCNT-GOD [41], 20 μM for GR-chitosan/GOD [42], and 0.5 μM for polyaniline/CNT/Pt/GOD [43]. "
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    ABSTRACT: In this paper, single-stranded DNA (ss-DNA) is demonstrated to functionalize graphene (GR) and to further guide the growth of PtAu bimetallic nanoparticles (PtAuNPs) on GR with high densities and dispersion. The obtained nanocomposites (PtAuNPs/ss-DNA/GR) were characterized by transmission electron microscopy (TEM), energy-dispersive X-ray spectrometer (EDS), and electrochemical techniques. Then, an enzyme nanoassembly was prepared by self-assembling glucose oxidase (GOD) on PtAuNP/ss-DNA/GR nanocomposites (GOD/PtAuNPs/ss-DNA/GR). The nanocomposites provided a suitable microenvironment for GOD to retain its biological activity. The direct and reversible electron transfer process between the active site of GOD and the modified electrode was realized without any extra electron mediator. Thus, the prepared GOD/PtAuNP/ss-DNA/GR electrode was proposed as a biosensor for the quantification of glucose. The effects of pH, applied potential, and temperature on the performance of the biosensor were discussed in detail and were optimized. Under optimal conditions, the biosensor showed a linearity with glucose concentration in the range of 1.0 to 1,800 muM with a detection limit of 0.3 muM (S/N = 3). The results demonstrate that the developed approach provides a promising strategy to improve the sensitivity and enzyme activity of electrochemical biosensors.
    Full-text · Article · Feb 2014 · Nanoscale Research Letters
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    • "mmol L −1 with the correlation coefficient of 0.981 and a fast current response time (within 4 s). The linear equation was I (␮A) = 3.224 C (mmol L −1 ) + 0.960 and the sensitivity of the biosensor was 45.6 ␮A/(mmol L −1 cm −2 ) which was higher than values in the literature [25] [35]. The response of biosensor to 0.1–1 mmol L −1 glucose is also studied with correlation coefficient of 0.962. "
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    ABSTRACT: A novel glucose biosensor was fabricated by integrating glucose oxidase (GOx) with chitosan/NiFe2O4 nanoparticles (CHIT/NiFe2O4NPs) on a glassy carbon electrode (GCE). The properties of CHIT/NiFe2O4NPs/GOx were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), UV–vis spectroscopy, Fourier transform infrared spectroscopy (FTIR) and electrochemical impedance spectroscopy (EIS). The GCE modified with the CHIT/NiFe2O4NPs/GOx showed excellent electrocatalytical response to the oxidation of glucose when ferrocene carboxylic acid was used as an artificial redox mediator, which was studied by cyclic voltammetry (CV). Different parameters including GOx concentration, working potential and pH of supporting electrolyte that governed the analytical performance of the biosensor, have been studied in detail and optimized. The biosensor was applied to detect glucose with a linear range of 1 × 10−4–2.0 × 10−2 mol L−1. The biosensor exhibited excellent performance for glucose at applied potential of 0.6 V with a fast response time (<4 s).
    Full-text · Article · Mar 2010 · Sensors and Actuators B Chemical
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    ABSTRACT: In this paper a highly sensitive glucose biosensor is proposed based on a polysilicon (poly-Si) wire structure coated with 3-aminopropyltriethoxysilane (γ-APTES) mixed with polydimethylsiloxane-treated hydrophobic fumed silica nanoparticles (NPs) as the sensing membrane. The γ-APTES and fumed silica NPs mixture was directly transferred to and coated onto the poly-Si wire region with the help of a focus-ion-beam (FIB) processed capillary atomic-force-microscope (C-AFM) tip. After the necessary curing and UV illumination processes, the resultant sensor showed an extremely wide linear detection range from 0.1μM to 10mM with a channel current sensitivity as high as 5.33AmM−1cm−2 (or a channel conductance sensitivity of 70μSmM−1), and a detection limit as low as 10nM can be achieved. Our experimental results showed that the poly-Si wire sensor has good selective analysis and operational stability on glucose detection under a 10:1 concentration ratio of glucose and uric acid. Its linear range and lowest detection limit remain virtually unimpaired in the presence of uric acid.
    No preview · Article · Oct 2009 · Sensors and Actuators B Chemical
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