Article

Efficacy of daily intake of Lactobacillus casei Shirota on respiratory symptoms and influenza vaccination immune response: A randomized, double-blind, placebo-controlled trial in healthy elderly nursing home residents

Department of Primary and Interdisciplinary Care, University of Antwerp, Antwerp, Belgium.
American Journal of Clinical Nutrition (Impact Factor: 6.77). 03/2012; 95(5):1165-71. DOI: 10.3945/ajcn.111.026831
Source: PubMed

ABSTRACT

Age is associated with immune dysregulation, which results in an increased infection rate and reduced effectiveness of vaccination.
We assessed whether an intervention with Lactobacillus casei Shirota (LcS) in elderly nursing home residents reduced their susceptibility to respiratory symptoms and improved their immune response to influenza vaccination.
Between October 2007 and April 2008, a randomized, double-blind, placebo-controlled trial was conducted in 737 healthy people aged ≥ 65 y in 53 nursing homes in Antwerp, Belgium. Volunteers were randomly assigned to receive a probiotic (n = 375; 2 bottles of fermented milk that contained ≥ 6.5 × 10(9) live LcS/bottle) or a placebo (n = 362; similar drink with no bacteria) for 176 d. After 21 d, all subjects received an influenza vaccination. Primary outcome parameters were the number of days with respiratory symptoms, the probability of respiratory symptoms, and antiinfluenza antibody titer by hemagglutination inhibition after vaccination.
Univariate and multivariate modeling showed no effect of the probiotic on clinical outcome parameters. Generalized linear mixed modeling showed no effect of the probiotic itself on the probability of respiratory symptoms [OR of probiotic: 0.8715; 95% CI: 0.6168, 1.2887). No significant difference regarding the influenza-vaccination immune response was shown.
The results of this study show that daily consumption of a fermented milk drink that contains LcS has no statistically or clinically significant effect on the protection against respiratory symptoms. This trial was registered at clinicaltrials.gov as NCT00849277.

Full-text preview

Available from: ajcn.nutrition.org
  • Source
    • "Mañé et al. [23] showed significant trends in reducing infection incidence and mortality due to pneumonia in institutionalized elderly subjects treated with two Lactobacillus plantarum strains. Some trials showed that Lactobacillus and Bifidobacterium probiotics could increase influenza vaccination immune responses in the elderly24252627. Endospore formers such as Bacillus species are interesting because their spores resist the acid barrier of the stomach and are stable for long periods in commercial food products [28]. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Bacillus probiotics health benefits have been until now quite poorly studied in the elderly population. This study aimed to assess the effects of Bacillus subtilis CU1 consumption on immune stimulation and resistance to common infectious disease (CID) episodes in healthy free-living seniors. One hundred subjects aged 60–74 were included in this randomized, double-blind, placebo-controlled, parallel-arms study. Subjects consumed either the placebo or the probiotic (2.10 9 B. subtilis CU1 spores daily) by short periodical courses of 10 days intermittently, alternating 18-day course of break. This scheme was repeated 4 times during the study. Symptoms of gastrointestinal and upper/lower respiratory tract infections were recorded daily by the subjects throughout the study (4 months). Blood, saliva and stool samples were collected in a predefined subset of the first forty-four subjects enrolled in the study. B. subtilis CU1 supplementation did not statistically significantly decrease the mean number of days of reported CID symptoms over the 4-month of study (probiotic group: 5.1 (7.0) d, placebo group: 6.6 (7.3) d, P = 0.2015). However, in the subset of forty-four randomized subjects providing biological samples, we showed that consumption of B. subtilis CU1 significantly increased fecal and salivary secretory IgA concentrations compared to the placebo. A post-hoc analysis on this subset showed a decreased frequency of respiratory infections in the probiotc group compared to the placebo group. Taken together, our study provides evidence that B. subtilis CU1 supplementation during the winter period may be a safe effective way to stimulate immune responses in elderly subjects.
    Full-text · Article · Dec 2015 · Immunity & Ageing
  • Source
    • "This is connected to the fact that the gut (or its associated lymphoid system, GALT) is the largest immunologically competent organ in the human body (de Vrese & Marteau, 2007;Eizaguirre et al., 2002). Probiotics are believed to positively influence the immune function (Ashraf, Vasiljevic, Day, Smith, & Donkor, 2014;Herrera, Salva, Villena, Barbieri, & Alvarez, 2013;Kim et al., 2015), and several studies reported immunomodulatory activity associated with LcS (Hori, Kiyoshima, Shida, & Yasui, 2001Matsuzaki, 1998;Nagao, Nakayama, Muto, & Okumura, 2000;Van Puyenbroeck et al., 2012). LcS is able to promote the innate immunity by increasing NK cell activity, and improves inflammatory status by an increasing IL-10/IL-12 ratio. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Lactobacillus casei Shirota (LcS) is a probiotic strain typically consumed with dairy products. In this study 142 hospitalized patients with symptoms of acute gastroenteritis were included. Patients in the LcS group received twice daily 65 ml of a commercial beverage containing L. casei Shirota. The treatment with LcS resulted in a significantly decreased daily average (−5.42 vs. −4.40) and cumulative rate of bowel movements (−32.49 vs. −26.43) and improvement of the glomerular filtration rate (after 24 hours: 41.9 ± 2.8 vs. 25.9 ± 4.2 ml/min, p < 0.01). Furthermore, treatment with LcS resulted in a significant decrease of CRP on days five, six and seven. Leukocyte counts decreased in all groups, while on day three the effect was significantly higher in the LcS group receiving antibiotics. In our study, L. casei Shirota administration twice a day had positive effects on the reduction of bowel movements, improvement of kidney function and inflammation compared to the control group. Hence, we would suggest that additional LcS administration might be considered in those patients with acute gastroenteritis who present with high inflammation markers and/or acute impaired kidney function.
    Full-text · Article · Aug 2015 · Journal of Functional Foods
  • [Show abstract] [Hide abstract]
    ABSTRACT: Probiotics have been proposed for a number of urogenital infectious conditions. In this study, we examine a possible effect on human papillomavirus (HPV)-related precancerous lesions in cervical cytology. We conducted a prospective controlled pilot study, in which 54 women with an HPV+low-grade squamous intraepithelial lesion diagnosis in their PAP smear were followed for 6 months. The intervention group consumed a daily probiotic drink during the study period; the control group received no treatment, according to common care policy. Outcome measures were the control PAP smear and HPV status after 6 months. Probiotic users had a twice as high chance of clearance of cytological abnormalities (60 vs. 31%, P=0.05). HPV was cleared in 19% of control patients versus 29% of probiotic users (P=0.41). This exploratory pilot study suggests that the probiotic studied promotes the clearance of HPV-related cytological abnormalities. If confirmed, this would represent an entirely new option to manage cervical cancer precursors.
    No preview · Article · Jun 2012 · European journal of cancer prevention: the official journal of the European Cancer Prevention Organisation (ECP)
Show more