Increased Migration of Monocytes in Essential Hypertension Is Associated with Increased Transient Receptor Potential Channel Canonical Type 3 Channels

Department of Hypertension and Endocrinology, Center for Hypertension and Metabolic Diseases, Daping Hospital, Third Military Medical University, Chongqing Institute of Hypertension, Chongqing, China.
PLoS ONE (Impact Factor: 3.23). 03/2012; 7(3):e32628. DOI: 10.1371/journal.pone.0032628
Source: PubMed


Increased transient receptor potential canonical type 3 (TRPC3) channels have been observed in patients with essential hypertension. In the present study we tested the hypothesis that increased monocyte migration is associated with increased TRPC3 expression. Monocyte migration assay was performed in a microchemotaxis chamber using chemoattractants formylated peptide Met-Leu-Phe (fMLP) and tumor necrosis factor-α (TNF-α). Proteins were identified by immunoblotting and quantitative in-cell Western assay. The effects of TRP channel-inhibitor 2-aminoethoxydiphenylborane (2-APB) and small interfering RNA knockdown of TRPC3 were investigated. We observed an increased fMLP-induced migration of monocytes from hypertensive patients compared with normotensive control subjects (246 ± 14% vs 151 ± 10%). The TNF-α-induced migration of monocytes in patients with essential hypertension was also significantly increased compared to normotensive control subjects (221 ± 20% vs 138 ± 18%). In the presence of 2-APB or after siRNA knockdown of TRPC3 the fMLP-induced monocyte migration was significantly blocked. The fMLP-induced changes of cytosolic calcium were significantly increased in monocytes from hypertensive patients compared to normotensive control subjects. The fMLP-induced monocyte migration was significantly reduced in the presence of inhibitors of tyrosine kinase and phosphoinositide 3-kinase. We conclude that increased monocyte migration in patients with essential hypertension is associated with increased TRPC3 channels.

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    • "Initially, Liu D. [14] observed a significant increase of TRPC3 protein expression in essential hypertensive patients compared with normotensive control subjects, which increase the strength of the calcium influx to aggravate the condition of hypertensive patients. Further research showed the monocyte migration function of patients was associated with an increased expression of TRPC3, while inhibiting TRPC3 molecules could prevent mononuclear cells after migration [15]. Compared TRPC3, -6 expression level in the monocytes of patients with primary hypertension and normal people, Liu and his teammates [16] once again confirmed that the mononuclear cells in patients with TRPC3 expression was increased, but TRPC6 expression had no change. "

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    • "Capsaicin, a TRPV1-specific agonist, increases migration in smooth muscle cells, corneal epithelial cells and hepatoblastoma cells treated with hepatocyte growth factor (Martin et al., 2012; Waning et al., 2007; Yang et al., 2010). In addition to TRPV1, other members of the TRP family, including TRPC and TRPM, can also influence cell motility and migration (Damann et al., 2009; Wei et al., 2009; Zhao et al., 2012). "
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